{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:34:52Z","timestamp":1762868092888,"version":"build-2065373602"},"reference-count":53,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":628,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1177\/2472555220909726","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T05:27:06Z","timestamp":1584595626000},"page":"634-645","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combined siRNA and Small-Molecule Phenotypic Screening Identifies Targets Regulating Rhinovirus Replication in Primary Human Bronchial Epithelial Cells"],"prefix":"10.1016","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8146-1535","authenticated-orcid":false,"given":"Mei","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Tyrchan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"B\u00e4ck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rgen","family":"\u00d6stling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steffen","family":"Schubert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"McCrae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1177\/2472555220909726_bib1","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/0042-6822(91)90098-V","article-title":"The Major and Minor Group Receptor Families Contain All but One Human Rhinovirus Serotype","volume":"180","author":"Uncapher","year":"1991","journal-title":"Virology"},{"key":"10.1177\/2472555220909726_bib2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1128\/CMR.00077-12","article-title":"Human Rhinoviruses","volume":"26","author":"Jacobs","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1177\/2472555220909726_bib3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.jaci.2005.06.003","article-title":"The Role of Rhinovirus in Asthma Exacerbations","volume":"116","author":"Friedlander","year":"2005","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1177\/2472555220909726_bib4","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/j.1398-9995.2010.02505.x","article-title":"Viruses and Bacteria in Acute Asthma Exacerbations\u2014A GA(2) LEN-DARE Systematic Review","volume":"66","author":"Papadopoulos","year":"2011","journal-title":"Allergy"},{"key":"10.1177\/2472555220909726_bib5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.3389\/fmicb.2013.00293","article-title":"Virus-Induced Exacerbations in Asthma and COPD","volume":"4","author":"Kurai","year":"2013","journal-title":"Front. Microbiol."},{"key":"10.1177\/2472555220909726_bib6","first-page":"405876","article-title":"Rhinovirus-Induced Exacerbations of Asthma and COPD","volume":"2013","author":"Hershenson","year":"2013","journal-title":"Scientifica (Cairo)"},{"key":"10.1177\/2472555220909726_bib7","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.1073\/pnas.1421178112","article-title":"Cadherin-Related Family Member 3, a Childhood Asthma Susceptibility Gene Product, Mediates Rhinovirus C Binding and Replication","volume":"112","author":"Bochkov","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1177\/2472555220909726_bib8","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/nchembio.2050","article-title":"Parallel shRNA and CRISPR-Cas9 Screens Enable Antiviral Drug Target Identification","volume":"12","author":"Deans","year":"2016","journal-title":"Nat. Chem. Biol."},{"key":"10.1177\/2472555220909726_bib9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1089\/adt.2012.475","article-title":"An Arrayed Genome-Scale Lentiviral-Enabled Short Hairpin RNA Screen Identifies Lethal and Rescuer Gene Candidates","volume":"11","author":"Bhinder","year":"2013","journal-title":"Assay Drug Dev. Technol."},{"key":"10.1177\/2472555220909726_bib10","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/nrd.2017.111","article-title":"Opportunities and Challenges in Phenotypic Drug Discovery: An Industry Perspective","volume":"16","author":"Moffat","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1177\/2472555220909726_bib11","doi-asserted-by":"crossref","first-page":"e0198714","DOI":"10.1371\/journal.pone.0198714","article-title":"Highly Multiplexed Genome Engineering Using CRISPR\/Cas9 gRNA Arrays","volume":"13","author":"Kurata","year":"2018","journal-title":"PLoS One"},{"key":"10.1177\/2472555220909726_bib12","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1073\/pnas.1012351108","article-title":"RNAi Screen of the Protein Kinome Identifies Checkpoint Kinase 1 (CHK1) as a Therapeutic Target in Neuroblastoma","volume":"108","author":"Cole","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1177\/2472555220909726_bib13","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1517\/14728220903179338","article-title":"RNA Interference Screening for the Discovery of Oncology Targets","volume":"13","author":"Quon","year":"2009","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1177\/2472555220909726_bib14","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.chom.2008.10.004","article-title":"Genome-Scale RNAi Screen for Host Factors Required for HIV Replication","volume":"4","author":"Zhou","year":"2008","journal-title":"Cell Host Microbe"},{"key":"10.1177\/2472555220909726_bib15","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1165\/rcmb.2017-0438OC","article-title":"Lanosterol Synthase Regulates Human Rhinovirus Replication in Human Bronchial Epithelial Cells","volume":"59","author":"McCrae","year":"2018","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"10.1177\/2472555220909726_bib16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nrd3010","article-title":"Recognizing and Avoiding siRNA Off-Target Effects for Target Identification and Therapeutic Application","volume":"9","author":"Jackson","year":"2010","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1177\/2472555220909726_bib17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev-biochem-060408-092949","article-title":"Genomic Screening with RNAi: Results and Challenges","volume":"79","author":"Mohr","year":"2010","journal-title":"Annu. Rev. Biochem."},{"key":"10.1177\/2472555220909726_bib18","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1021\/cb100358f","article-title":"Vigilance and Validation: Keys to Success in RNAi Screening","volume":"6","author":"Sigoillot","year":"2011","journal-title":"ACS Chem. Biol."},{"key":"10.1177\/2472555220909726_bib19","doi-asserted-by":"crossref","first-page":"eaww8412","DOI":"10.1126\/scitranslmed.aaw8412","article-title":"Off-Target Toxicity Is a Common Mechanism of Action of Cancer Drugs Undergoing Clinical Trials","volume":"11","author":"Lin","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"10.1177\/2472555220909726_bib20","first-page":"227","article-title":"The Method of \u201cRight and Wrong Cases\u201d (Constant Stimuli) without Gauss\u2019s Formula","volume":"2","author":"Spearman","year":"1908","journal-title":"Br. J. Psychol."},{"key":"10.1177\/2472555220909726_bib21","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1007\/BF01863914","article-title":"Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche","volume":"162","author":"K\u00e4rber","year":"1931","journal-title":"Naunyn Schmiedebergs Arch. Exp. Pathol. Pharmakol."},{"key":"10.1177\/2472555220909726_bib22","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1080\/019262301317052440","article-title":"Toxicologic Lesions Associated with Two Related Inhibitors of Oxidosqualene Cyclase in the Dog and Mouse","volume":"29","author":"Pyrah","year":"2001","journal-title":"Toxicol. Pathol."},{"key":"10.1177\/2472555220909726_bib23","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1186\/s12985-015-0330-4","article-title":"In Vitro Characterisation of a Pleconaril\/Pirodavir-Like Compound with Potent Activity against Rhinoviruses","volume":"12","author":"Lacroix","year":"2015","journal-title":"Virol. J."},{"key":"10.1177\/2472555220909726_bib24","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1021\/ci0504723","article-title":"Multifingerprint Based Similarity Searches for Targeted Class Compound Selection","volume":"46","author":"Kogej","year":"2006","journal-title":"J. Chem. Inf. Model."},{"key":"10.1177\/2472555220909726_bib25","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1021\/ci800326z","article-title":"Comparison of Molecular Fingerprint Methods on the Basis of Biological Profile Data","volume":"49","author":"Steffen","year":"2009","journal-title":"J. Chem. Inf. Model."},{"year":"2017","series-title":"BIOVIA Workbook, Release 2017; BIOVIA Pipeline Pilot, Release 2017","key":"10.1177\/2472555220909726_bib26"},{"key":"10.1177\/2472555220909726_bib27","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/nmeth1089","article-title":"A Probability-Based Approach for the Analysis of Large-Scale RNAi Screens","volume":"4","author":"Konig","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1177\/2472555220909726_bib28","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nrd4336","article-title":"The Discovery of First-in-Class Drugs: Origins and Evolution","volume":"13","author":"Eder","year":"2014","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1177\/2472555220909726_bib29","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nrd3480","article-title":"How Were New Medicines Discovered?","volume":"10","author":"Swinney","year":"2011","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1177\/2472555220909726_bib30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/1752-0509-2-49","article-title":"Systems Analysis of Quantitative shRNA-Library Screens Identifies Regulators of Cell Adhesion","volume":"2","author":"Huang","year":"2008","journal-title":"BMC Syst. Biol."},{"key":"10.1177\/2472555220909726_bib31","doi-asserted-by":"crossref","first-page":"5250","DOI":"10.1093\/nar\/gkn506","article-title":"A Functional Genome-Wide RNAi Screen Identifies TAF1 as a Regulator for Apoptosis in Response to Genotoxic Stress","volume":"36","author":"Kimura","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1177\/2472555220909726_bib32","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1126\/science.1149200","article-title":"Cancer Proliferation Gene Discovery through Functional Genomics","volume":"319","author":"Schlabach","year":"2008","journal-title":"Science"},{"key":"10.1177\/2472555220909726_bib33","doi-asserted-by":"crossref","first-page":"12577","DOI":"10.1074\/jbc.M117.775155","article-title":"An RNAi-Based High-Throughput Screening Assay to Identify Small Molecule Inhibitors of Hepatitis B Virus Replication","volume":"292","author":"Ghosh","year":"2017","journal-title":"J. Biol. Chem."},{"key":"10.1177\/2472555220909726_bib34","doi-asserted-by":"crossref","first-page":"4527","DOI":"10.1093\/nar\/gki762","article-title":"siRNA-Mediated Off-Target Gene Silencing Triggered by a 7 nt Complementation","volume":"33","author":"Lin","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1177\/2472555220909726_bib35","doi-asserted-by":"crossref","first-page":"e2003213","DOI":"10.1371\/journal.pbio.2003213","article-title":"Evaluation of RNAi and CRISPR Technologies by Large-Scale Gene Expression Profiling in the Connectivity Map","volume":"15","author":"Smith","year":"2017","journal-title":"PLoS Biol."},{"key":"10.1177\/2472555220909726_bib36","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1021\/acschembio.9b00075","article-title":"A Phenotypic Screening Approach Using Human Treg Cells Identified Regulators of Forkhead Box p3 Expression","volume":"14","author":"Ding","year":"2019","journal-title":"ACS Chem. Biol."},{"key":"10.1177\/2472555220909726_bib37","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1517\/14728222.10.2.337","article-title":"RNAi: For Functional Analysis and Target Validation","volume":"10","author":"Rondinone","year":"2006","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1177\/2472555220909726_bib38","doi-asserted-by":"crossref","first-page":"e1007601","DOI":"10.1371\/journal.ppat.1007601","article-title":"RNAi-Based Small Molecule Repositioning Reveals Clinically Approved Urea-Based Kinase Inhibitors as Broadly Active Antivirals","volume":"15","author":"Lesch","year":"2019","journal-title":"PLoS Pathog."},{"key":"10.1177\/2472555220909726_bib39","doi-asserted-by":"crossref","unstructured":"Chen J., Du F., Dang Y.; et al. FGF19-Mediated Upregulation of SOX18 Promotes Hepatocellular Carcinoma Metastasis by Transactivating FGFR4 and FLT4. Hepatology [Online early access.] DOI: 10.1002\/hep.30951. Published Online: Sept 16, 2019.","DOI":"10.1002\/hep.30951"},{"key":"10.1177\/2472555220909726_bib40","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1358\/dot.2019.55.8.3010573","article-title":"Erdafitinib to Treat Urothelial Carcinoma","volume":"55","author":"Hanna","year":"2019","journal-title":"Drugs Today (Barc.)"},{"key":"10.1177\/2472555220909726_bib41","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1002\/psp4.12210","article-title":"Population Modeling Integrating Pharmacokinetics, Pharmacodynamics, Pharmacogenetics, and Clinical Outcome in Patients with Sunitinib-Treated Cancer","volume":"6","author":"Diekstra","year":"2017","journal-title":"CPT Pharmacometrics Syst. Pharmacol."},{"key":"10.1177\/2472555220909726_bib42","doi-asserted-by":"crossref","first-page":"2678","DOI":"10.1158\/1078-0432.CCR-17-3512","article-title":"Telatinib Is an Effective Targeted Therapy for Pseudomyogenic Hemangioendothelioma","volume":"24","author":"van IJzendoorn","year":"2018","journal-title":"Clin. Cancer Res."},{"key":"10.1177\/2472555220909726_bib43","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1634\/theoncologist.2014-0177","volume":"20","author":"Tzogani","year":"2015","journal-title":"Oncologist"},{"key":"10.1177\/2472555220909726_bib44","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/j.jaci.2008.01.067","article-title":"Human Rhinovirus Infection Enhances Airway Epithelial Cell Production of Growth Factors Involved in Airway Remodeling","volume":"121","author":"Leigh","year":"2008","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1177\/2472555220909726_bib45","doi-asserted-by":"crossref","first-page":"i653","DOI":"10.1093\/bioinformatics\/btq398","article-title":"Detecting Host Factors Involved in Virus Infection by Observing the Clustering of Infected Cells in siRNA Screening Images","volume":"26","author":"Suratanee","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1177\/2472555220909726_bib46","doi-asserted-by":"crossref","first-page":"11320","DOI":"10.1038\/ncomms11320","article-title":"A Human Genome-Wide Loss-of-Function Screen Identifies Effective Chikungunya Antiviral Drugs","volume":"7","author":"Karlas","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1177\/2472555220909726_bib47","doi-asserted-by":"crossref","first-page":"2724","DOI":"10.3389\/fmicb.2018.02724","article-title":"Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact with trVLP Elements","volume":"9","author":"Yu","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1177\/2472555220909726_bib48","doi-asserted-by":"crossref","first-page":"6758","DOI":"10.1128\/JVI.03241-12","article-title":"Ets-1 Is Required for the Activation of VEGFR3 during Latent Kaposi\u2019s Sarcoma-Associated Herpesvirus Infection of Endothelial Cells","volume":"87","author":"Gutierrez","year":"2013","journal-title":"J. Virol."},{"key":"10.1177\/2472555220909726_bib49","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1164\/rccm.201008-1276OC","article-title":"RIG-Like Helicase Innate Immunity Inhibits Vascular Endothelial Growth Factor Tissue Responses via a Type I IFN-Dependent Mechanism","volume":"183","author":"Ma","year":"2011","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1177\/2472555220909726_bib50","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1084\/jem.20180861","article-title":"Hes1 Attenuates Type I IFN Responses via VEGF-C and WDFY1","volume":"216","author":"Ning","year":"2019","journal-title":"J. Exp. Med."},{"key":"10.1177\/2472555220909726_bib51","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1046\/j.1365-2222.2003.01576.x","article-title":"Increased Levels of Vascular Endothelial Growth Factor in Induced Sputum in Asthmatic Patients","volume":"33","author":"Asai","year":"2003","journal-title":"Clin. Exp. Allergy"},{"key":"10.1177\/2472555220909726_bib52","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1067\/mai.2001.111928","article-title":"Expression of Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Angiogenin Immunoreactivity in Asthmatic Airways and Its Relationship to Angiogenesis","volume":"107","author":"Hoshino","year":"2001","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1177\/2472555220909726_bib53","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/resp.13142","article-title":"VEGF Synthesis Is Induced by Prostacyclin and TGF-beta in Distal Lung Fibroblasts from COPD Patients and Control Subjects: Implications for Pulmonary Vascular Remodelling","volume":"23","author":"Westergren-Thorsson","year":"2018","journal-title":"Respirology"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/2472555220909726","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/2472555220909726","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222065704?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2472555222065704?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/2472555220909726","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:51:47Z","timestamp":1762861907000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2472555222065704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["S2472555222065704"],"URL":"https:\/\/doi.org\/10.1177\/2472555220909726","relation":{},"ISSN":["2472-5552"],"issn-type":[{"type":"print","value":"2472-5552"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Combined siRNA and Small-Molecule Phenotypic Screening Identifies Targets Regulating Rhinovirus Replication in Primary Human Bronchial Epithelial Cells","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1177\/2472555220909726","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2020 Society for Laboratory Automation and Screening. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}