{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:35:27Z","timestamp":1793054127781,"version":"4.5.0"},"reference-count":69,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Bengbu Medical University","award":["2022byzd062"],"award-info":[{"award-number":["2022byzd062"]}]},{"name":"Foundation of Educational Department in Anhui province","award":["2022AH051433"],"award-info":[{"award-number":["2022AH051433"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ophthalmol Eye Dis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Primary open-angle glaucoma (POAG) is a leading cause of vision loss, with lipid metabolism implicated in its pathogenesis. Our study explores lipid metabolism-derived acylation modifications in POAG using GEO, GWAS, and PubChem databases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Integrated multi-omics approaches were employed: Mendelian randomization assessed causal relationships between lipid traits and POAG risk; transcriptomic analysis (gene set variation analysis, weighted gene co-expression network analysis) identified key acylation-related genes; machine learning selected feature genes; and single-cell sequencing validated mechanisms in human trabecular meshwork samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Four lipid metabolism-derived acylations (palmitoylation, myristoylation, succinylation, malonylation) were significantly upregulated in POAG. Core genes 5\u2032-aminolevulinate synthase 2 (ALAS2) and phospholipase C epsilon 1 (PLCE1) were mechanistically linked: ALAS2 catalyzed acyl-CoA accumulation, while myristoylated PLCE1 promoted trabecular fibrosis via collagen type IV alpha 3 chain interaction. External datasets confirmed these findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Lipid-derived acylations drive POAG through ALAS2-mediated acyl-CoA production and PLCE1-induced fibrosis, revealing novel targets for intervention. Lipid metabolism-derived acylation modifications, particularly succinylation, propionylation, and myristoylation, may play a crucial role in POAG. These findings highlight the potential role of acylation modifications in POAG and offer new insights into POAG\u2019s molecular mechanisms and potential research directions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/25158414251405373","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T10:26:44Z","timestamp":1765880804000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Multi-omics analysis unveils the role of lipid metabolism-derived acylation modifications in POAG and its potential implications"],"prefix":"10.1177","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8089-4324","authenticated-orcid":false,"given":"Sijie","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China"},{"name":"Jinan University Faculty of Medical Science, Guangzhou, Guangdong Province, China"},{"name":"Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China"},{"name":"Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqing","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province 510632, China"},{"name":"Jinan University Faculty of Medical Science, Guangzhou, Guangdong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100916"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(99)00019-1"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.3192"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(02)00014-9"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2015.09.005"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16257-0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2019.08.002"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.328.7431.97"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2022.11.030"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-022-2344-5"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-40233-2"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.173935"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19020926"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2022.2762"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-45872-z"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.63.13.3"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)01289-8"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2024.109788"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-81048-3"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu12030878"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043925"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2010.11.022"},{"issue":"7","key":"e_1_3_4_24_2","first-page":"2224","article-title":"Intraocular pressure in an American community. 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