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We performed an epigenome-wide longitudinal DNAm study of a well-characterized sample of young women from the Swedish Born into Life study, with repeated blood sampling before, during and after pregnancy (n\u2009=\u200921), using the Illumina Infinium MethylationEPIC array. We conducted a replication in the Isle of Wight third-generation birth cohort (n\u2009=\u200927), using the Infinium HumanMethylation450k BeadChip. We identified 196 CpG sites displaying intra-individual longitudinal change in DNAm with a false discovery rate (FDR) P\u2009&lt;\u2009.05. Most of these (91%) showed a decrease in average methylation levels over the studied period. We observed several genes represented by \u2a7e3 differentially methylated CpGs: HOXB3, AVP, LOC100996291, and MicroRNA 10a. Of 36 CpGs available in the replication cohort, 17 were replicated, all but 2 with the same direction of association (replication P\u2009&lt;\u2009.05). Biological pathway analysis demonstrated that FDR-significant CpGs belong to genes overrepresented in metabolism-related pathways, such as adipose tissue development, regulation of insulin receptor signaling, and mammary gland fat development. These results contribute to a better understanding of the biological mechanisms underlying important physiological alterations and adaptations for pregnancy and lactation.<\/jats:p>","DOI":"10.1177\/2516865719867090","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T02:57:41Z","timestamp":1565751461000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":42,"title":["DNA Methylation Trajectories During Pregnancy"],"prefix":"10.1177","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4147-5654","authenticated-orcid":false,"given":"Olena","family":"Gruzieva","sequence":"first","affiliation":[{"name":"Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden"},{"name":"Centre for Occupational and Environmental Medicine, Stockholm County Council, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon 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