{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:38:22Z","timestamp":1777646302751,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["T32NR009759"],"award-info":[{"award-number":["T32NR009759"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["F32NR014622"],"award-info":[{"award-number":["F32NR014622"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P01HD30367"],"award-info":[{"award-number":["P01HD30367"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004060","name":"Preeclampsia Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genet Epigenet"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>We compared blood-based DNA methylation levels of endoglin ( ENG) and transforming growth factor beta receptor 2 ( TGF\u03b2R2) gene promoter regions between women with clinically-overt preeclampsia and women with uncomplicated, normotensive pregnancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We used EpiTect Methyl II PCR Assays to evaluate DNA methylation of CpG islands located in promoter regions of ENG (CpG Island 114642) and TGF\u03b2R2 (CpG Island 110111). Preeclampsia was diagnosed based on blood pressure, protein, and uric acid criteria. N\u2009=\u200921 nulliparous preeclampsia case participants were 1:1 frequency matched to N\u2009=\u200921 nulliparous normotensive control participants on gestational age at sample collection (\u00b12\u2009weeks), smoking status, and labor status at sample collection. Methylation values were compared between case and control participant groups [( ENG subset: n\u2009=\u200920 (9 cases, 11 controls); TGF\u03b2R2 subset: n\u2009=\u200928 (15 cases, 13 controls)].<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The majority of the preeclampsia cases delivered at \u2a7e34\u2009weeks\u2019 gestation (83%). Average methylation levels for ENG ([M\u2009\u00b1\u2009(SD)]; Case Participant Group\u2009=\u20096.54%\u2009\u00b1\u20094.57 versus Control Participant group\u2009=\u20094.81%\u2009\u00b1\u20095.08; P\u2009=\u2009.102) and TGF\u03b2R2 (Case Participant Group\u2009=\u20091.50%\u2009\u00b1\u20091.37 vs Control Participant Group\u2009=\u20091.70%\u2009\u00b1\u20091.40; P\u2009=\u2009.695) promoter CpG islands did not differ significantly between the participant groups. Removal of 2 extreme outliers in the ENG analytic subset revealed a trend between levels of ENG methylation and pregnancy outcome (Case Participant Group\u2009=\u20095.17%\u2009\u00b1\u20092.16 vs Control Participant Group\u2009=\u20093.36%\u2009\u00b1\u20091.73; P\u2009=\u2009.062).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Additional epigenetic studies that include larger sample sizes, investigate preeclampsia subtypes, and capture methylation status of CpG island shores and shelves are needed to further inform us of the potential role that ENG and TGF\u03b2R2 DNA methylation plays in preeclampsia pathophysiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/2516865720959682","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T04:08:44Z","timestamp":1602216524000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["DNA Methylation of Endoglin Pathway Genes in Pregnant Women With and Without Preeclampsia"],"prefix":"10.1177","volume":"13","author":[{"given":"Allison H","family":"Rietze","sequence":"first","affiliation":[{"name":"Department of Health Promotion and Development (School of Nursing), University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvette P","family":"Conley","sequence":"additional","affiliation":[{"name":"Department of Health Promotion and Development (School of Nursing), University of Pittsburgh, Pittsburgh, PA, USA"},{"name":"Department of Human Genetics (Graduate School of Public Health), University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dianxu","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Health and Community Systems (School of Nursing), University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy M","family":"Anderson","sequence":"additional","affiliation":[{"name":"Martha S Pitzer Center for Women, Children, and Youth (College of Nursing), The Ohio State University, Columbus, OH, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, & Reproductive Sciences (School of Medicine), University of Pittsburgh, PA, USA"},{"name":"Magee-Womens Research Institute, Pittsburgh, PA, USA"},{"name":"Department of Epidemiology (Graduate School of Public Health), University of Pittsburgh, Pittsburgh, PA, USA"},{"name":"Clinical and Translational Science Institute, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Jeyabalan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, & Reproductive Sciences (School of Medicine), University of Pittsburgh, PA, USA"},{"name":"Magee-Womens Research Institute, Pittsburgh, PA, USA"},{"name":"Clinical and Translational Science Institute, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl A","family":"Hubel","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, & Reproductive Sciences (School of Medicine), University of Pittsburgh, PA, USA"},{"name":"Magee-Womens Research Institute, Pittsburgh, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandy J","family":"Schmella","sequence":"additional","affiliation":[{"name":"Department of Health Promotion and 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