{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T05:43:51Z","timestamp":1786167831794,"version":"build-2736575974"},"reference-count":71,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The CRISPR Journal"],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:p>\n                    Craniosynostosis is a rare congenital bone condition where skull sutures fuse prematurely and is linked to mutations in over 60 genes. Generating mutation-specific\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    models allows investigation of craniosynostosis-associated mutations without the need for patient-derived material or transgenic gene expression. Here, we developed a human\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    disease model with the CRISPR-Cas9 prime editing variant, using an immortalized TERT-immortalized mesenchymal bone marrow-derived stem (MSC-TERT) cell line with osteogenic potential. MSC-TERT cells showed a higher resistance to prime editing, compared with HEK293FT cells. Addition of dnMLH1 and epegRNAs resulted in higher editing efficiencies in HEK293FT cells, but not in MSC-TERT cells. Prime editing efficiency varied between targeted loci and was found to be more efficient in nonadherent cells compared with adherent cells. Prime editing continued over 4 days in an isolated nonadherent HEK293FT culture. Our results present a foundation on the use of prime editing to establish\n                    <jats:italic toggle=\"yes\">FGFR2<\/jats:italic>\n                    mutation-specific\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    models and their application in MSC-TERT cells.\n                  <\/jats:p>","DOI":"10.1177\/25731599261457618","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T08:08:47Z","timestamp":1782461327000},"page":"191-206","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Prime Editing Efficiency in Human Immortalized MSC-TERT Cells with Osteogenic Potential for Modeling\n                    <i>FGFR2<\/i>\n                    -Linked Craniosynostosis"],"prefix":"10.1177","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8646-814X","authenticated-orcid":false,"given":"Max","family":"Gijsbertsen","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8322-5669","authenticated-orcid":false,"given":"Filipa M.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."},{"name":"Department of Plastic and Reconstructive Surgery and Hand Surgery, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8967-8260","authenticated-orcid":false,"given":"Ana","family":"Fuentes Manj\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiany","family":"Maduro","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1557-0869","authenticated-orcid":false,"given":"Moustapha","family":"Kassem","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5024-1871","authenticated-orcid":false,"given":"John","family":"van der Oost","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Research, Wageningen University, Wageningen, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1675-9922","authenticated-orcid":false,"given":"Irene M.J.","family":"Mathijssen","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery and Hand Surgery, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2282-4006","authenticated-orcid":false,"given":"Johannes P.T.M.","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6524-727X","authenticated-orcid":false,"given":"Jeroen","family":"van de Peppel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Medical Center Rotterdam, Erasmus MC, Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"issue":"1","key":"e_1_3_2_2_1","first-page":"5","article-title":"Genetics of craniosynostosis: Review of the literature","volume":"2","author":"Ciurea AV","year":"2009","unstructured":"1.Ciurea AV, , Toader C. Genetics of craniosynostosis: Review of the literature. J Med Life 2009;2(1):5\u201317.","journal-title":"J Med Life"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320470507"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3340\/jkns.2016.59.3.192"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00475.x"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1597\/1545-1569(2000)037<0433:TCDSCS>2.0.CO;2"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ocr.12153"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0000000000000542"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spen.2007.08.008"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.10.1647"},{"key":"e_1_3_2_11_1","volume-title":"GeneReviews\u00ae","author":"Wenger T","year":"1993","unstructured":"10.Wenger T, , Miller D, , Evans K, et al. FGFR Craniosynostosis Syndromes Overview. In: GeneReviews\u00ae. (Adam MP, , Feldman J, , Mirzaa Pagon, , GM RA, , Wallace SE, , Bean LJ eds) University of Washington, Seattle: Seattle, WA; 1993. 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