{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:51:05Z","timestamp":1791471065331,"version":"4.3.4"},"reference-count":26,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The CRISPR Journal"],"abstract":"<jats:p>\n                    Dog allergy is a prevalent IgE-mediated condition, with the salivary lipocalin\n                    <jats:italic toggle=\"yes\">Can f<\/jats:italic>\n                    1 accounting for the majority of dog-specific IgE reactivity in sensitized individuals. Existing strategies for managing dog allergy primarily rely on modulating host immune responses and do not address allergen production at its source. Here, we report the generation of genetically engineered dogs lacking\n                    <jats:italic toggle=\"yes\">Can f<\/jats:italic>\n                    1. Using CRISPR-Cas9 editing and somatic cell nuclear transfer, we produced two healthy beagle puppies carrying a frameshift mutation in exon 1 of the\n                    <jats:italic toggle=\"yes\">Can f<\/jats:italic>\n                    1 gene. Salivary and hair\/dander analysis demonstrated the absence of\n                    <jats:italic toggle=\"yes\">Can f<\/jats:italic>\n                    1 protein in the edited dogs. Skin prick testing in a sensitized individual demonstrated robust IgE-mediated reactivity to control dog extracts but no detectable response to extracts from the edited dogs. Together, these findings demonstrate that targeted genetic knockout of the major dog allergen is compatible with canine development and can abolish the IgE-mediated allergic response, supporting the feasibility of a gene-based approach to reducing canine allergenicity.\n                  <\/jats:p>","DOI":"10.1177\/25731599261473124","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:00:27Z","timestamp":1785927627000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Targeted Genetic Knockout of\n                    <i>Can f<\/i>\n                    1, the Major Allergen in Dogs"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3345-8248","authenticated-orcid":false,"given":"Matt W.G.","family":"Walker","sequence":"first","affiliation":[{"name":"Kindred Companion Sciences, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Margolis","sequence":"additional","affiliation":[{"name":"Kindred Companion Sciences, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanner","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Kindred Companion Sciences, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Gavin","sequence":"additional","affiliation":[{"name":"Kindred Companion Sciences, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Loewy","sequence":"additional","affiliation":[{"name":"Comprehensive Allergy Clinic, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.2201035"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00386.x"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14216"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13391"},{"issue":"4","key":"e_1_3_3_6_1","first-page":"188","article-title":"Why is pet (cat\/dog) Allergen Immunotherapy (AIT) such a controversial topic? Current perspectives and future directions","volume":"56","author":"Liccardi G","year":"2024","unstructured":"5.Liccardi G, , Martini M, , Bil\u00f2 MB, et al. Why is pet (cat\/dog) Allergen Immunotherapy (AIT) such a controversial topic? Current perspectives and future directions. Eur Ann Allergy Clin Immunol 2024;56(4):188\u2013191; doi: 10.23822\/eurannaci.1764-1489.330","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.08.019"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.05.013"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2012.02.015"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.919155"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136690"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjv061"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-012-0283-2"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2021.09.008"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/385810a0"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/436641a"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-022-00749-3"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz365"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003118"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts378"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu393"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2012.02.020"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.12.640"}],"container-title":["The CRISPR Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/25731599261473124","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/25731599261473124","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/25731599261473124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T16:46:32Z","timestamp":1786207592000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/25731599261473124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":26,"alternative-id":["10.1177\/25731599261473124"],"URL":"https:\/\/doi.org\/10.1177\/25731599261473124","relation":{},"ISSN":["2573-1599","2573-1602"],"issn-type":[{"value":"2573-1599","type":"print"},{"value":"2573-1602","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]},"article-number":"25731599261473124"}}