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This review outlines the transformation of DNA glycosylases from unwanted side-effect generators into core drivers of base editor evolution. Through strategic protein engineering, glycosylases have been harnessed to create novel editors that mediate crucial transversion edits, substantially expanding the targeting landscape of precision genome editing. We will examine how these engineered glycosylases achieve programmable DNA rewriting through redirected DNA repair pathways, compare emerging and conventional editors, and explore applications in genetic therapy and functional genomics while addressing ongoing challenges in specificity and delivery. Finally, we envision next-generation editors equipped with artificial intelligence-designed glycosylases capable of spatiotemporally controlled genome manipulation.<\/jats:p>","DOI":"10.1177\/25731599261475668","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:48:07Z","timestamp":1790581687000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Addressing the Blind Spots of Deaminase Base Editors with CRISPR-Cas Glycosylase-Based Editors"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7211-1680","authenticated-orcid":false,"given":"Jian","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicines, The First Affiliated Hospital of Xi\u2019an Medical University, Xian, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae419"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-024-01212-3"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-0084-6"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17946"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24644"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59653-x"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf281"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21559-9"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-00450-9"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2024.01.021"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49343-5"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac353"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c11269"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01486-w"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21464-1"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2025.03.001"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-50012-w"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-02050-w"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59948-z"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25217-y"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38193-2"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf717"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25375-z"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-00938-z"},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01821-9"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01595-6"},{"key":"e_1_3_2_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202309004"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01532-7"},{"key":"e_1_3_2_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-01163-8"},{"key":"e_1_3_2_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0609-x"},{"key":"e_1_3_2_32_1","article-title":"Development of B-to-A base editors by leveraging translesion synthesis polymerase","author":"Liang P","year":"2025","unstructured":"31.Liang P, , Zhang P, , Chen Y, et al. 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