{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:45:05Z","timestamp":1792064705426,"version":"4.4.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"11-12","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Photobiomodulation, Photomedicine, and Laser Surgery"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      are frequently co-isolated from refractory endodontic infections. Antimicrobial photodynamic therapy (aPDT) is a promising adjunctive disinfection strategy; however, its efficacy against polymicrobial communities containing structurally distinct organisms remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      To evaluate methylene blue\u2013mediated aPDT at three energy densities (15, 30, and 60 J\/cm\n                      <jats:sup>2<\/jats:sup>\n                      ) against a dual-species coculture model and to investigate the ecological consequences of differential species susceptibility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Dual-species biofilms were established on silicone substrates. Mature cocultures were treated with 100 \u00b5M methylene blue and irradiated with 630 nm light-emitting diode (LED) light. Morphological changes were assessed by scanning electron microscopy (SEM); bacterial and fungal viability were quantified by colony forming unit assays on species-selective media.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      aPDT produced dose-dependent structural damage to\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      . SEM showed approximately 90% morphological reduction, and viable counts fell by approximately 1.95 log\n                      <jats:sub>10<\/jats:sub>\n                      (\u224899%) at 60 J\/cm\n                      <jats:sup>2<\/jats:sup>\n                      .\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      remained morphologically intact across all energy densities. Selective, dose-dependent bacterial inactivation, confirmed by viable counts, was accompanied by secondary fungal overgrowth.\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      proliferation rates reached 460\u2013690% within 18 h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Differential species susceptibility to methylene blue\u2013mediated aPDT disrupts microbial competitive equilibrium in polymicrobial communities. Optimized aPDT protocols for polymicrobial endodontic infections should incorporate antifungal components to prevent secondary fungal proliferation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/25785478261481597","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T02:14:51Z","timestamp":1787192091000},"page":"626-632","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial Photodynamic Therapy Reveals Differential Species Susceptibility and Ecological Shifts in\n                    <i>Candida albicans\u2013Pseudomonas aeruginosa<\/i>\n                    Mixed Model"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3372-0727","authenticated-orcid":false,"given":"Jiahe","family":"Li","sequence":"first","affiliation":[{"name":"Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13677"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-023-04947-x"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0255003"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8091300"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2023.102368"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.911623"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-11"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-00901-5"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.017673"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-023-03938-4"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.02.009"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/b311900a"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01299"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30268-7"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103292"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11112883"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2711"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00796-9"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom5010142"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137750"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.53"}],"container-title":["Photobiomodulation, Photomedicine, and Laser Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/25785478261481597","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/25785478261481597","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/25785478261481597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:47:21Z","timestamp":1792061241000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/25785478261481597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":21,"journal-issue":{"issue":"11-12","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10.1177\/25785478261481597"],"URL":"https:\/\/doi.org\/10.1177\/25785478261481597","relation":{},"ISSN":["2578-5478","2578-5478"],"issn-type":[{"value":"2578-5478","type":"print"},{"value":"2578-5478","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}