{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:54:08Z","timestamp":1790074448622,"version":"4.0.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[1978,12,1]],"date-time":"1978-12-01T00:00:00Z","timestamp":281318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1978,12]]},"abstract":"<jats:p>An immunocytochemical technique for the demonstration of intracellular antigens (secretory proteins) on thin sections is reported. Staphylococcal protein A which reacts with the Fc fragment of IgG molecules was labeled with colloidal gold as a marker. The antigenic sites were visualized on aldehyde-fixed and Epon-embedded tissue in a two step procedure. The specific antisera were applied to thin sections for binding to the antigens and then visualized by the protein A-gold complex. By using this technique different secretory proteins of the exocrine and endocrine pancreas were localized. The protein A-gold technique is proposed as a general method for visualization of antigenic sites on thin sections.<\/jats:p>","DOI":"10.1177\/26.12.366014","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T01:19:10Z","timestamp":1301620750000},"page":"1074-1081","source":"Crossref","is-referenced-by-count":662,"title":["Ultrastructural localization of intracellular antigens by the use of protein A-gold complex."],"prefix":"10.1177","volume":"26","author":[{"given":"J","family":"Roth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bendayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Orci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1978,12,1]]},"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/26.12.366014","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/26.12.366014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:22:39Z","timestamp":1777501359000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/26.12.366014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1978,12]]}},"alternative-id":["10.1177\/26.12.366014"],"URL":"https:\/\/doi.org\/10.1177\/26.12.366014","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,12]]}}}