{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:28:42Z","timestamp":1787124522890,"version":"build-2736575974"},"reference-count":23,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["PHAGE: Therapy, Applications, and Research"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"\n \n Background:<\/jats:italic>\n <\/jats:title>\n \n Bacteriophages serve as natural biocontrol agents and are viable alternatives to antibiotics for the management of bacterial infections in aquaculture.\n Aeromonas hydrophila<\/jats:italic>\n , a significant fish pathogen, has developed multidrug resistance.\n <\/jats:p>\n <\/jats:sec>\n \n \n Material and Method:<\/jats:italic>\n <\/jats:title>\n \n This study aimed to isolate and characterize a lytic bacteriophage targeting\n A. hydrophila<\/jats:italic>\n .\n AhP_Mru1<\/jats:italic>\n , a novel lytic bacteriophage against\n A. hydrophila<\/jats:italic>\n , was isolated from the Pawana River in Pune, Maharashtra, India. Using conventional enrichment and plaque purification procedures, spot tests, and transmission electron microscopy, we revealed its tailed morphology with an icosahedral capsid. Whole-genome sequencing revealed that\n AhP_Mru1<\/jats:italic>\n has a 117-kb double-stranded DNA genome with a high GC content and encodes genes for DNA replication, metabolism, and host lysis but lacks integrase or toxin genes.\n <\/jats:p>\n <\/jats:sec>\n \n \n Result:<\/jats:italic>\n <\/jats:title>\n \n Comparative genomic analysis showed <95% similarity to closely related\n Aeromonas<\/jats:italic>\n phages, and phylogenetic analyses placed it in the genus\n Shenzhenvirus<\/jats:italic>\n of the family\n Demerecviridae<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n \n Conclusion:<\/jats:italic>\n <\/jats:title>\n \n AhP_Mru1<\/jats:italic>\n genomic features and taxonomic position distinguish it as a novel phage species.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/26416549261448091","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T13:40:08Z","timestamp":1779543608000},"page":"219-226","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and Physiological Growth Kinetics Analysis of a Novel Lytic\n Aeromonas Phage AhP_Mru1<\/i>\n Against\n Aeromonas hydrophila<\/i>"],"prefix":"10.1177","volume":"7","author":[{"given":"Mrunalini","family":"Sonne","sequence":"first","affiliation":[{"name":"AK. Kulkarni, Department of Biodiversity, M.E.S. Abasaheb Garware College, Pune, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivedita","family":"Ghayal","sequence":"additional","affiliation":[{"name":"Department of Botany, M.E.S. Abasaheb Garware College, Pune, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2601-661X","authenticated-orcid":false,"given":"Sangeeta","family":"Ahiwale","sequence":"additional","affiliation":[{"name":"Department of Microbiology, R.S.S. Mahatma Phule College, Pimpari, Pune, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,23]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00100-6"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.05.002"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14112498"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13071268"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-016-1166-x"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.25991"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8010129"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00039-09"},{"key":"e_1_3_3_10_1","volume-title":"Molecular cloning: A laboratory manual","author":"Sambrook J","year":"2001","unstructured":"9.Sambrook J, , Russell DW. 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WorldFish, Bayan Lepas, Malaysia. 2013."},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2021.1143-1152"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2017.803-807"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.22.3.365"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-164-6_7"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765x.1997.tb00005.x"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-018-1588-8"},{"key":"e_1_3_3_19_1","first-page":"1","article-title":"An insight into the therapeutic potential of a novel lytic Pseudomonas phage isolated from the river Ganga","author":"Nisha R","year":"2022","unstructured":"18.Nisha R, , Chandan K, , Bimal KD, , Rama C. An insight into the therapeutic potential of a novel lytic Pseudomonas phage isolated from the river Ganga. J Appl Microbiol 2022:1\u201310.","journal-title":"J Appl Microbiol"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2012-0589"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-164-6_18"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_24_1","doi-asserted-by":"crossref","unstructured":"23.VIRIDIC\u2014A Novel Tool to Calculate the Intergenomic Similarities of Prokaryote-Infecting Viruses. 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