{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:28:40Z","timestamp":1787124520463,"version":"build-2736575974"},"reference-count":56,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100006206","name":"Biological and Environmental Research","doi-asserted-by":"crossref","award":["DE-AC02-05CH11231"],"award-info":[{"award-number":["DE-AC02-05CH11231"]}],"id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["PHAGE: Therapy, Applications, and Research"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                      is a common bacterial pathogen in nosocomial infections. It has become one of the greatest threats to human health for its growing resistance to \u201clast resort\u201d antibiotics, which has led to a revival of phage therapy as a potential treatment. However, conventional methods for isolating\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      -infecting phages are labor-intensive and often unsuccessful.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Our approach involves a computational pipeline to identify temperate phages (prophages) integrated into\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      genomes, followed by mitomycin C (MMC) induction of those strains to screen for active prophages.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Here we show a prophage analysis for nearly 900\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      genomes. We observed MMC-triggered excision of nine prophages from eight\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      strains by Polymerase Chain Reaction (PCR) and sequencing. Further, we show four prophage-formed virions detectable by transmission electron microscopy and two that can plaque on other\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      This work demonstrates the utility and diversity of prophages for further development as therapeutics for antibiotic-resistant\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  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