{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T14:04:14Z","timestamp":1784988254727,"version":"3.55.0"},"reference-count":67,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["PHAGE: Therapy, Applications, and Research"],"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Antimicrobial resistance poses a global health threat, particularly in low- and middle-income countries. Multidrug-resistant\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      is one of the most common pathogens causing hospital-acquired infections and death. The Phages for Global Health initiative promotes the capacity to develop bacteriophages as a promising therapeutic alternative targeting clinically relevant pathogens as a solution for antibiotic-resistant infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results:<\/jats:title>\n                    <jats:p>\n                      Sewage from Al-Azhar Hospital was used to isolate a novel lytic\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      phage, vB-EcoM-Eg05. Transmission electron microscopy identified vB-EcoM-Eg05 as a myovirus. The phage showed high stability up to 60\u00b0C and a broad pH range (3\u201311). Whole-genome sequencing revealed a 77-gene genome, including 1 transfer RNA and 41 genes with predicted functions. Comparative genomics indicated that phage vB-EcoM-Eg05 is a new species in the genus\n                      <jats:italic toggle=\"yes\">Carltongylesvirus<\/jats:italic>\n                      . No undesirable genes were detected in bioinformatic analyses, confirming that the phage is safe and that it is a strictly lytic phage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      Phage vB-EcoM-Eg05 has therapeutic potential for further development against clinical\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/26416549261465081","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:45:04Z","timestamp":1784691904000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Therapeutic Potential of vB-EcoM-Eg05 Phage Against Clinical\n                    <i>Escherichia coli<\/i>\n                    Strains"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2941-0612","authenticated-orcid":false,"given":"Shimaa","family":"Badawy","sequence":"first","affiliation":[{"name":"Department of Bacteriology and Immunology, Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland."},{"name":"Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samaa","family":"Shama","sequence":"additional","affiliation":[{"name":"Bioscience Research Laboratories Department, MARC for Medical Services and Scientific Research, Giza, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Skurnik","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00135-18"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2020.100673"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-9668.184707"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2020.100924"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.11.004"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/ejhpharm-2016-001013"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-022-07678-8"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1202215"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2019.104433"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(25)00118-5"},{"key":"e_1_3_3_12_1","first-page":"65","article-title":"Bad bugs, no drugs 2002\u20132020: Progress, challenges, and call to action","volume":"131","author":"Boucher HW","year":"2020","unstructured":"11.Boucher HW. Bad bugs, no drugs 2002\u20132020: Progress, challenges, and call to action. 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