{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T08:19:47Z","timestamp":1777882787006,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Women's Health Reports"],"published-print":{"date-parts":[[2025,4,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To determine the presence or absence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in second-trimester amniotic fluid during the SARS-CoV-2 virus pandemic in patients undergoing genetic amniocentesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design:<\/jats:title>\n                    <jats:p>\n                      Women undergoing amniocentesis between September 2020 and April 2022 were asked to participate in the study. On the day of amniocentesis, an additional 5 mL of amniotic fluid, 5 mL of maternal blood sample, and nasal swabs were collected from each participant. None of the patients had active SARS-CoV-2 infection on the date of amniocentesis. Demographic data and history of SARS-CoV-2 virus infection were collected. After 2021, the SARS-CoV-2 virus vaccination status was obtained. Amniotic fluid and maternal nasal swabs were collected and tested for the presence of the SARS-CoV-2 virus\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      real-time reverse-transcriptase polymerase chain reaction test using an automated bead-based method for extracting total nucleic acid. An electrochemiluminescence immunoassay on maternal blood was used to detect SARS-CoV-2 total (immunoglobin G\/immunoglobin M) antibodies against nucleocapsids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>This study enrolled 92 patients. There were 16 patients who reported a history of SARS-CoV-2 virus infection: 8 of them reported SARS-CoV-2 virus infection during the first and second trimesters and 8 reported SARS-CoV-2 virus infection before pregnancy. A total of 49 patients were vaccinated prior to amniocentesis. All 92 amniotic fluid samples tested negative for the SARS-CoV-2 virus. There were five positive and three negative SARS-CoV-2 maternal nucleocapsid antibody tests among the eight patients who had the SARS-COV-2 viral infection during the first and second trimesters of pregnancy. All eight patients had negative swab tests for the SARS-CoV-2 virus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>There was no SARS-CoV-2 virus in the second-trimester amniotic fluid samples from the eight patients who reported SARS-CoV-2 viral infection during the first and second trimesters. This finding suggests that, after a first- and second-trimester SARS-CoV-2 virus infection, it is unlikely to find the SARS-CoV-2 virus in the amniotic fluid.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/26884844251387022","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T14:57:41Z","timestamp":1760021861000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Absence of SARS-CoV-2 in Second-Trimester Amniotic Fluid During the SARS-CoV-2 Virus Pandemic"],"prefix":"10.1177","volume":"6","author":[{"given":"Poonam","family":"Samyal","sequence":"first","affiliation":[{"name":"Comprehensive Perinatal Medicine, PLLC, Flushing, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Bracero","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, South Shore University Hospital, Bay Shore, New York, USA."},{"name":"Zucker School of Medicine at Hofstra\/Northwell, New Hyde Park, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Quinn","sequence":"additional","affiliation":[{"name":"Zucker School of Medicine at Hofstra\/Northwell, New Hyde Park, New York, USA."},{"name":"Department of Obstetrics and Gynecology, Long Island Jewish Medical Center, New Hyde Park, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Chan","sequence":"additional","affiliation":[{"name":"Comprehensive Perinatal Medicine, PLLC, Flushing, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_3_6_3_2","unstructured":"World Health Organization. WHO Director-General\u2019s opening remarks at the media briefing on COVID-19 - 11 March 2020. World Health Organization; 2020."},{"key":"e_1_3_6_4_2","unstructured":"World Health Organization. Definition and categorization of the timing of mother-to-child transmission of SARS-CoV-2. World Health Organization; 2021."},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10225253"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.04.014"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.21037\/tp-20-144"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2021-067696"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-021-06049-z"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.04.039"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0250196"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18115709"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2022.08.009"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0270893"},{"issue":"5","key":"e_1_3_6_15_2","first-page":"1048","article-title":"A prospective study on vertical transmission of SARS-CoV-2 in pregnancy in a tertiary referral center in South India","volume":"14","author":"James P","year":"2023","unstructured":"James P, , Rajan R, , Devi KLS, et al. A prospective study on vertical transmission of SARS-CoV-2 in pregnancy in a tertiary referral center in South India. JCDR, 2023; 14(5):1048\u20131053; doi: 10.31838\/jcdr.2023.14.05.127","journal-title":"JCDR"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10102152"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2020.1811669"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.11.019"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.112.4.e254"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.07.049"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2023.11.329"}],"container-title":["Women's Health Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/26884844251387022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/26884844251387022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/26884844251387022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:23:26Z","timestamp":1777598606000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/26884844251387022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,4,1]]}},"alternative-id":["10.1177\/26884844251387022"],"URL":"https:\/\/doi.org\/10.1177\/26884844251387022","relation":{},"ISSN":["2688-4844","2688-4844"],"issn-type":[{"value":"2688-4844","type":"print"},{"value":"2688-4844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,1]]},"article-number":"26884844261425162"}}