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However, its effects have not been confirmed by in vivo studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study was aimed at evaluating the hepatoprotective effect of a methanolic extract of\n                      <jats:italic toggle=\"yes\">Bersama abyssinica<\/jats:italic>\n                      leaf on paracetamol-induced hepatotoxicity in Swiss albino mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study was conducted on thirty male Swiss albino mice, randomly grouped into six groups of five mice each; normal, negative, and positive control groups received 1\u00a0mL\/kg of distilled water, paracetamol 250\u00a0mg\/kg only, and silymarin (100\u00a0mg\/kg) orally with paracetamol, respectively. While treatment groups I, II, and III received 200, 400, and 600\u00a0mg\/kg of the\n                      <jats:italic toggle=\"yes\">Bersama abyssinica<\/jats:italic>\n                      leaf methanolic extract with paracetamol 250\u00a0mg\/kg, respectively. At the end of the experiment, the mice were sacrificed after being anesthetized, and blood samples were collected via cardiac puncture, and the liver was removed for histopathological investigation. Data were entered into Epi Data version 3.1 and exported to SPSS version 25 for further analysis using a one-way ANOVA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The negative control group showed higher levels of ALT, AST, ALP, GGT, and bilirubin compared to the normal control. However, significant hepatoprotective effects were noted in the treatment and positive control groups, as evidenced by the substantially reduced levels of these liver biomarkers towards the normal range. The significantly lower levels of indirect bilirubin were seen among treatment groups II and III as compared to group II. Abnormal liver architectures were observed in the negative control group; however, these changes were reversed following administration of the leaf extract.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The study found that the methanolic leaf extract of\n                      <jats:italic toggle=\"yes\">Bersama abyssinica<\/jats:italic>\n                      exhibited hepatoprotective effects against paracetamol-induced liver injury.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/27536130251405227","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T11:13:16Z","timestamp":1764155596000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Hepatoprotective Effect of\n                    <i>Bersama abyssinica Fresen<\/i>\n                    Leaf Methanolic Extract on Paracetamol-Induced Hepatotoxicity in Swiss Albino Mice"],"prefix":"10.1177","volume":"14","author":[{"given":"Shashitu","family":"Mekuria","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Debre Markos University, Debre Markos, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tesaka","family":"Wondimnew","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Jimma University, Jimma, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minale","family":"Fekadie","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Jimma University, Jimma, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abebe Dukessa","family":"Dubiwak","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Jimma University, Jimma, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1468-4640","authenticated-orcid":false,"given":"Nuredin Chura","family":"Waritu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Wolaita Sodo University, Wolaita Sodo, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuji","family":"Kasim","sequence":"additional","affiliation":[{"name":"School of Medicine, Shashemene Comprehensive Specialized referral hospital, Oromia, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Jemal","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine, Debre Markos University, Debre Markos, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.03.017"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(23)00570-3"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-03186-x"},{"key":"e_1_3_6_5_2","first-page":"95","article-title":"Hepatotoxicity of paracetamol and related fatalities","volume":"21","author":"Tittarelli R","year":"2017","unstructured":"Tittarelli R, Pellegrini M, Scarpellini MG, et al. 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