{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:41:04Z","timestamp":1777851664843,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[1980,6,1]],"date-time":"1980-06-01T00:00:00Z","timestamp":328665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:p>Tissue amino groups undergo dithiocarbamylation on treatment (12 hr, 22-25 degrees C) with a carbon disulfide:triethylamine:tetrahydrofuran (1:1:0.5, v\/v) mixture. After washing with tetrahydrofuran:triethylamine (4:1, v\/v) followed by tetrahydrofuran:pyridine (4:1,v\/v) and ice-cold distilled water tissues were immersed into ice-cold 10% (w\/v) lead acetate to yield yellowish lead dithiocarbamates. At 22-25 degrees C the derivatives obtained from primary amines decompose to lead sulfide. After washing with 5% lead acetate in 10% acetic acid followed by three changes of distilled water tissues are dehydrated and embedded in paraffin or Maraglas as usual. In proteins the reaction is given by the epsilon-amino group of lysine and alpha-terminal amino groups, while nucleic acids do not react. Results are shown in tetrahydrofuran-delipidized ethanol or formalin-fixed 1) plant root tips where brown-colored and electron-dense lead sulfide deposits are localized in condensed chromatin and chromosomes attributable to lysine-rich histones and 2) rat skeletal muscle where an interfibrillar component is visualized in a repeating pattern, possibly related to terminal regions of the sarcoplasmic reticulum.<\/jats:p>","DOI":"10.1177\/28.6.7391548","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T02:04:08Z","timestamp":1301623448000},"page":"499-506","source":"Crossref","is-referenced-by-count":2,"title":["Dithiocarbamylation in histochemistry: carbon disulfide as a reagent for the visualization of primary amino groups with the light and electron microscope."],"prefix":"10.1177","volume":"28","author":[{"given":"C J","family":"Tandler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1980,6,1]]},"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/28.6.7391548","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/28.6.7391548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:24:27Z","timestamp":1777501467000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/28.6.7391548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1980,6]]}},"alternative-id":["10.1177\/28.6.7391548"],"URL":"https:\/\/doi.org\/10.1177\/28.6.7391548","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,6]]}}}