{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:22:20Z","timestamp":1777443740003,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T00:00:00Z","timestamp":1568332800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Scientific and Technological Research Council of Turkey-TUBITAK 1001 Project","award":["116Z294"],"award-info":[{"award-number":["116Z294"]}]},{"name":"Coimbra Chemistry Centre (CQC) is supported by FCT","award":["UI0313\/QUI\/2013"],"award-info":[{"award-number":["UI0313\/QUI\/2013"]}]},{"name":"General Directorate of Agricultural Research and Policies","award":["TAGEM\/16\/AR-GE\/56"],"award-info":[{"award-number":["TAGEM\/16\/AR-GE\/56"]}]},{"name":"by FEDER\/COMPETE 2020-EU","award":["UI0313\/QUI\/2013"],"award-info":[{"award-number":["UI0313\/QUI\/2013"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Spectrosc"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    Seeds belonging to fourth generation mutants (M\n                    <jats:sub>4<\/jats:sub>\n                    ) of Ataem-7 cultivar (A7) variety and S04-05 (S) breeding line salt-tolerant soybeans were investigated by Raman spectroscopy, complemented by chemometrics methods, in order to evaluate changes induced by mutations in the relative lipid\u2013protein contents, and to find fast, efficient strategies for discrimination of the mutants and the control groups based on their Raman spectra. It was concluded that gamma irradiation caused an increase in the lipid to protein ratio of the studied Ataem-7 variety mutants, while it led to a decrease of this ratio in the investigated S04-05 breeding line mutants. These results were found to be in agreement with data obtained by reflectance spectrum analysis of the seeds in the full ultraviolet to near-infrared spectral region and suggest the possibility of developing strategies where gamma irradiation can be used as a tool to improve mutant soybean plants targeted to different applications, either enriched in proteins or in lipids. Ward's clustering and principal component analysis showed a clear discrimination between mutants and controls and, in the case of the studied S-type species, discrimination between the different mutants. The grouping scheme is also found to be in agreement with the compositional information extracted from the analysis of the lipid\u2013protein contents of the different samples.\n                  <\/jats:p>","DOI":"10.1177\/0003702819859940","type":"journal-article","created":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T09:10:21Z","timestamp":1561021821000},"page":"34-41","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Raman Spectroscopic and Chemometric Investigation of Lipid\u2013Protein Ratio Contents of Soybean Mutants"],"prefix":"10.1177","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7827-5050","authenticated-orcid":false,"given":"Gulce","family":"Ogruc Ildiz","sequence":"first","affiliation":[{"name":"Department of Physics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"},{"name":"CQC, Department of Chemistry, University of Coimbra, Coimbra, Portugal"}]},{"given":"Ozge","family":"Celik","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"}]},{"given":"Cimen","family":"Atak","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"}]},{"given":"Ayberk","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Physics, Science Faculty, Istanbul University, Istanbul, Turkey"}]},{"given":"Hayrunnisa Nur","family":"Kabuk","sequence":"additional","affiliation":[{"name":"Department of Physics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"}]},{"given":"Ersin","family":"Kaygisiz","sequence":"additional","affiliation":[{"name":"Department of Physics, Science Faculty, Istanbul University, Istanbul, Turkey"}]},{"given":"Alp","family":"Ayan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"}]},{"given":"Sinan","family":"Meric","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Science and Letters Faculty, Istanbul Kultur University, Istanbul, Turkey"}]},{"given":"Rui","family":"Fausto","sequence":"additional","affiliation":[{"name":"CQC, Department of Chemistry, University of Coimbra, Coimbra, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2019,9,13]]},"reference":[{"key":"bibr1-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-893997-64-6.50008-1"},{"key":"bibr2-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1007\/s40003-013-0088-0"},{"key":"bibr3-0003702819859940","volume-title":"Genetic Resources, Chromosome Engineering, and Crop Improvement: Oilseed Crops","volume":"4","author":"Singh R.J.","year":"2007"},{"key":"bibr4-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4330-7"},{"key":"bibr5-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2013.11.042"},{"key":"bibr6-0003702819859940","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex269"},{"key":"bibr7-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/423590"},{"key":"bibr8-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1590\/1516-635X1604437-442"},{"key":"bibr9-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2013.04.103"},{"key":"bibr10-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1021\/jf301247w"},{"key":"bibr11-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2018.03.007"},{"key":"bibr12-0003702819859940","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572012000600015"},{"issue":"2","key":"bibr13-0003702819859940","first-page":"1","volume":"18","author":"Zhang B.","year":"2011","journal-title":"J. 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