{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:23:51Z","timestamp":1777443831760,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T00:00:00Z","timestamp":1415836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Decreased serum concentrations of vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      are associated with Alzheimer\u2019s type dementia. The transcobalamin II gene (TCN2) 776C\u2192G polymorphism affects transcobalamin II function as a carrier of vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      and might modify its availability. The association of the TCN2 776C\u2192G polymorphism with Alzheimer\u2019s type dementia is unclear and was investigated in the present study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Case\u2013control study including 27 individuals diagnosed with Alzheimer\u2019s type dementia and 28 healthy controls. Serum concentrations of vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      , homocysteine and other analytes were determined and the presence of TCN2 776C\u2192G and 5, 10-methylenetetrahydrofolate reductase 1298A\u2192C polymorphisms genotypes was ascertained by polymerase chain reaction\u2013restriction fragment length polymorphism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Serum concentrations of vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      were lower while those of homocysteine were higher in patients than in controls ( P\u2009&lt;\u20090.05). The frequency of individuals carrying at least one 5, 10-methylenetetrahydrofolate reductase 1298C allele was higher (59% versus 32%) while frequency of individuals harbouring at least one TCN2 776G allele was lower (58% versus 86%) in patients than in controls ( P\u2009&lt;\u20090.05). Univariate logistic regression showed negative association of TCN2 776CG genotype with Alzheimer\u2019s type dementia (OR\u2009=\u20090.17 versus CC genotype, P\u2009&lt;\u20090.02). Multivariate logistic regression identified TCN2 776C\u2192G polymorphism as independent predictor of Alzheimer\u2019s type dementia together with higher concentrations of homocysteine, cholesterol and uric acid and lower concentrations of oestradiol. Association of TCN2 776C\u2192G polymorphism with Alzheimer\u2019s type dementia was observed for individuals carrying the 5,10-methylenetetrahydrofolate reductase 1298AA genotype but not the AC or CC genotypes, indicating interaction between the two polymorphisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The TCN2 776C\u2192G polymorphism is negatively associated with Alzheimer\u2019s type dementia, suggesting a protective role against the disease in subjects with the 5, 10-methylenetetrahydrofolate reductase 1298AA genotype.<\/jats:p>\n                  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Portugal"}]},{"given":"Madalena","family":"Barroso","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.UL), Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"}]},{"given":"Rita","family":"Castro","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.UL), Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"},{"name":"Department of Biochemistry and Human Biology, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"}]},{"given":"Manuela","family":"Palmeira","sequence":"additional","affiliation":[{"name":"Cova da Beira Hospital Centre, Covilh\u00e3, Portugal"}]},{"given":"Andr\u00e9","family":"Serpa","sequence":"additional","affiliation":[{"name":"CICS-UBI \u2013 Health Sciences Research Centre, University of Beira Interior, Covilh\u00e3, Portugal"}]},{"given":"Ana C","family":"Dias-Cabral","sequence":"additional","affiliation":[{"name":"CICS-UBI \u2013 Health Sciences Research Centre, University of Beira Interior, Covilh\u00e3, Portugal"}]},{"given":"Fernanda C","family":"Domingues","sequence":"additional","affiliation":[{"name":"CICS-UBI \u2013 Health Sciences Research Centre, University of Beira Interior, Covilh\u00e3, Portugal"}]},{"given":"Sofia","family":"Almeida","sequence":"additional","affiliation":[{"name":"Cova da Beira Hospital Centre, Covilh\u00e3, 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