{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:49:55Z","timestamp":1777812595140,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T00:00:00Z","timestamp":1522627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>\n                    This investigation aimed at developing micropatterned silica thin films (MSTFs) containing nanohydroxyapatite (nano-HA) microaggregates that were not completely covered by silica so that they could directly interact with the surrounding cells. The objectives were 1) to evaluate the effect of the presence of 2 films (MSTF with or without nano-HA addition) on the characteristic strength (\u03c3\n                    <jats:sub>0<\/jats:sub>\n                    ) and Weibull modulus ( m) of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) and 2) to evaluate the effect of these 2 films, as applied onto the Y-TZP surface, on the morphology, orientation, and proliferation of MG63 cells. Sol-gel process and soft lithography were used to apply the MSTF onto the Y-TZP specimens. Three experimental groups were produced: Y-TZP, Y-TZP + MSTF, and Y-TZP + MSTF + sprayed nano-HA. All surfaces were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy and tested for 4-point flexural strength ( n = 30) in water at 37 \u00b0C. Weibull analysis was used to determine m and \u03c3\n                    <jats:sub>0<\/jats:sub>\n                    (maximum likelihood method). In vitro biological behavior was performed with human osteoblast-like cells (MG63). Y-TZP was successfully coated with MSFT and MSFT + nano-HA. Scanning electron microscopy micrographs indicated that the microaggregates of nano-HA were not entirely covered by the silica. There was no statistically significant difference among the experimental groups for \u03c3\n                    <jats:sub>0<\/jats:sub>\n                    and m. In the groups containing the films, the cells were elongated and aligned along the lines. The MSFT + nano-HA group showed significantly higher cell metabolic activity than that obtained for the Y-TZP group at day 7. This investigation was successful in producing an MSTF containing nano-HA microaggregates that remained exposed to the environment. The developed films did not jeopardize the structural reliability of a commercial Y-TZP, as confirmed by the Weibull statistics. The MG63 cells seeded over the films became elongated and aligned along the films\u2019 micropatterned lines. Y-TZP specimens coated with MSTF and nano-HA showed a higher cell metabolic activity and proliferation after 7 d of culture when compared with uncoated Y-TZP.\n                  <\/jats:p>","DOI":"10.1177\/0022034518765762","type":"journal-article","created":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T18:48:59Z","timestamp":1522694939000},"page":"1003-1009","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants"],"prefix":"10.1177","volume":"97","author":[{"given":"R.B.P.","family":"Miranda","sequence":"first","affiliation":[{"name":"Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brasil"},{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, U. Porto, Porto, Portugal"},{"name":"Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dent\u00e1ria, U. Porto, Porto, Portugal"}]},{"given":"L.","family":"Grenho","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dent\u00e1ria, U. Porto, Porto, Portugal"},{"name":"LAQV\/REQUIMTE, U. Porto, Portugal"}]},{"given":"A.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, U. Porto, Porto, Portugal"},{"name":"Departamento de Engenharia Metal\u00fargica e dos Materiais, Faculdade de Engenharia, U. Porto, Porto, Portugal"},{"name":"INEB\u2013Instituto de Engenharia Biom\u00e9dica, U. Porto, Porto, Portugal"}]},{"given":"M.H.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dent\u00e1ria, U. Porto, Porto, Portugal"},{"name":"LAQV\/REQUIMTE, U. Porto, Portugal"}]},{"given":"F.J.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, U. Porto, Porto, Portugal"},{"name":"Departamento de Engenharia Metal\u00fargica e dos Materiais, Faculdade de Engenharia, U. Porto, Porto, Portugal"},{"name":"INEB\u2013Instituto de Engenharia Biom\u00e9dica, U. Porto, Porto, Portugal"}]},{"given":"P.F.","family":"Cesar","sequence":"additional","affiliation":[{"name":"Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brasil"}]}],"member":"179","published-online":{"date-parts":[[2018,4,2]]},"reference":[{"key":"bibr1-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1515\/bmt-2015-0139"},{"key":"bibr2-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200805179"},{"key":"bibr3-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2012.09.002"},{"key":"bibr4-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.5b03955"},{"key":"bibr5-0022034518765762","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.15.19"},{"key":"bibr6-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.10.031"},{"key":"bibr7-0022034518765762","doi-asserted-by":"publisher","DOI":"10.3390\/ma10030257"},{"issue":"3","key":"bibr8-0022034518765762","first-page":"408","volume":"19","author":"Fugazzotto PA","year":"2004","journal-title":"Int J Oral Maxillofac Implants"},{"key":"bibr9-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnoncrysol.2004.08.172"},{"key":"bibr10-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1038\/258703a0"},{"key":"bibr11-0022034518765762","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1853-9"},{"issue":"2","key":"bibr12-0022034518765762","first-page":"184","volume":"12","author":"Heydecke G","year":"1999","journal-title":"Int J Prosthodont"},{"key":"bibr13-0022034518765762","doi-asserted-by":"publisher","DOI":"10.4012\/dmj.2015-129"},{"key":"bibr14-0022034518765762","unstructured":"International Organization for Standardization. 2015. 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