{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:41:58Z","timestamp":1777851718394,"version":"3.51.4"},"reference-count":53,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:p>Mucin O-glycosylation in cancer is characterized by aberrant expression of immature carbohydrate structures leading to exposure of simple mucin-type carbohydrate antigens and peptide epitopes. Glycosyltransferases controlling the initial steps of mucin O-glycosylation are responsible for the altered glycosylation observed in cancer. We studied the expression in gastric cell lines of six UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-T1, T2, T3, T4, T6, T11) that catalyze the initial key step in the regulation of mucin O-glycosylation, the transfer of GalNAc from UDP-GalNAc to serine and threonine residues. We also studied the expression of ST6GalNAc-I, the enzyme responsible for the synthesis of Sialyl-Tn antigen (NeuAc\u03b12,6GalNAc) and the ST3Gal-I, the enzyme responsible for the synthesis of Sialyl-T antigen (NeuAc\u03b12,3Gal\u03b21,3GalNAc). This study was done using specific monoclonal antibodies, enzymatic assays, and RT-PCR. Our results showed that GalNAc-T1, -T2, and -T3 have an ubiquitous expression in all gastric cell lines, whereas GalNAc-T4, -T6, and -T11 show a restricted expression pattern. The immunoreactivity with MAb VU-2-G7 suggests that, apart from GalNAc-T4, another GalNAc transferase is involved in the glycosylation of the Thr in the PDTR region of the MUC1 tandem repeat. The expression of ST3Gal-I correlates with the expression of the Sialyl-T antigen in gastric cell lines and in the control cell lines studied. The expression of ST6GalNAc-I is low in gastric cell lines, in accordance with the low\/absent expression of the Sialyl-Tn antigen.<\/jats:p>","DOI":"10.1177\/002215540305100607","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:28:21Z","timestamp":1301628501000},"page":"761-771","source":"Crossref","is-referenced-by-count":44,"title":["Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I Expression in Gastric Carcinoma Cell Lines"],"prefix":"10.1177","volume":"51","author":[{"given":"Nuno T.","family":"Marcos","sequence":"first","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal"}]},{"given":"Andrea","family":"Cruz","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal"}]},{"given":"Filipe","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal"}]},{"given":"Raquel","family":"Almeida","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal"}]},{"given":"Leonor","family":"David","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal"},{"name":"Medical Faculty of the University of Porto, Porto, Portugal"}]},{"given":"Ulla","family":"Mandel","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnostics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark"}]},{"given":"Henrik","family":"Clausen","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnostics, School of 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