{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:32:29Z","timestamp":1770910349601,"version":"3.50.1"},"reference-count":36,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p> The intracellular localization of cystic fibrosis transmembrane conductance regulator (CFTR) in native tissues is a major issue in the study of mutation, processing, and trafficking effects in CFTR and in the evaluation of therapeutic strategies in cystic fibrosis (CF). This work evaluated the applicability of ten different antibodies (Abs) under various fixation techniques for CFTR localization in fresh-brushed nasal epithelial cells collected from CF patients homozygous for F508del and control individuals. In parallel, the same Ab panel was also tested on BHK cell lines overexpressing wild-type or F508del CFTR. The Abs MATG1061, 169, Lis1, MP-CT1, CC24-R, MAB25031, and MAB1660 gave the best detection of CFTR in the apical region (AR) of nasal tall columnar epithelial (TCE) cells. The labeling pattern of these Abs was consistent with the postulated processing defect of F508del CFTR because only a minority of CF TCE cells present CFTR in the AR. In contrast, M3A7, MM13\u20134, and L12B4 weakly react with the AR and stain almost exclusively a cis-Golgi-like structure in the majority of CF and non-CF airway cells. In BHK cells, all the Abs enabled distinction between wild-type CFTR localization in cell membrane from F508del CFTR, which in these cells is exclusively located in the endoplasmic reticulum. <\/jats:p>","DOI":"10.1177\/002215540405200207","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T07:28:21Z","timestamp":1301642901000},"page":"193-203","source":"Crossref","is-referenced-by-count":40,"title":["CFTR Localization in Native Airway Cells and Cell Lines Expressing Wild-type or F508del-CFTR by a Panel of Different Antibodies"],"prefix":"10.1177","volume":"52","author":[{"given":"Isabel","family":"Carvalho-Oliveira","sequence":"first","affiliation":[{"name":"Centro de Gen\u00e9tica Humana, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Lisboa, Portugal"}]},{"given":"Alexandra","family":"Efthymiadou","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Athens, Aghia Sophia Children's Hospital, Athens, Greece"}]},{"given":"Rui","family":"Malh\u00f3","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Vegetal, Faculdade de Ci\u00eancias de Lisboa, Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Paulo","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Observat\u00f3rio Nacional de Sa\u00fade, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Lisboa, Portugal"}]},{"given":"Maria","family":"Tzetis","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Athens, Aghia Sophia Children's Hospital, Athens, Greece"}]},{"given":"Emmanuel","family":"Kanavakis","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, University of Athens, Aghia Sophia Children's Hospital, Athens, Greece"}]},{"given":"Margarida D.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias de Lisboa, Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Deborah","family":"Penque","sequence":"additional","affiliation":[{"name":"Centro de Gen\u00e9tica Humana, Instituto Nacional de Sa\u00fade Dr Ricardo Jorge, Lisboa, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2004,2,1]]},"reference":[{"key":"bibr1-002215540405200207","first-page":"191","volume":"72","author":"Brezillon S","year":"1995","journal-title":"Lab Invest"},{"key":"bibr2-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1007\/BF02633211"},{"key":"bibr3-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000022221.88025.43"},{"key":"bibr4-002215540405200207","doi-asserted-by":"crossref","first-page":"11304","DOI":"10.1016\/S0021-9258(18)82125-1","volume":"268","author":"Chang XB","year":"1993","journal-title":"J Biol Chem"},{"key":"bibr5-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.96.09122474"},{"key":"bibr6-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90148-8"},{"key":"bibr7-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1177\/002215540004800611"},{"key":"bibr8-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1126\/science.1375392"},{"key":"bibr9-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1177\/32.2.6198355"},{"key":"bibr10-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.20.9262"},{"key":"bibr11-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.153.1.8542144"},{"key":"bibr12-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115582"},{"key":"bibr13-002215540405200207","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.1242\/jcs.114.22.4073","volume":"114","author":"Dormer RL","year":"2001","journal-title":"J Cell Sci"},{"issue":"1","key":"bibr14-002215540405200207","first-page":"S117","volume":"443","author":"Dormer RL","year":"2001","journal-title":"Pflugers Arch"},{"key":"bibr15-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1996)8:2<134::AID-HUMU5>3.0.CO;2-B"},{"key":"bibr16-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118199"},{"issue":"1","key":"bibr17-002215540405200207","first-page":"165","volume":"44","author":"Engelhardt JF","year":"1992","journal-title":"J Pharm Pharmacol"},{"key":"bibr18-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1042\/bj20011717"},{"key":"bibr19-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33825"},{"key":"bibr20-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1038\/347382a0"},{"key":"bibr21-002215540405200207","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21873"},{"key":"bibr22-002215540405200207","unstructured":"Harris CM, McPherson M, Dormer RL. 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