{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:33:30Z","timestamp":1777923210072,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T00:00:00Z","timestamp":1607212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Waste Manag Res"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>\n                    The increasing concentration of livestock farms results in large amounts of waste production and the need for their management. The study of anaerobic digestion (AD) technology, under mesophilic conditions, applied to pig slurry is of the upmost importance for biogas recovery and sanitised digestate, contributing to a circular economy. The assessment of the effects of a feast\/famine regime on biogas and biomethane (bio-CH\n                    <jats:sub>4<\/jats:sub>\n                    ) yield with different feeding frequencies was performed. The evaluation was made in regards to three scenarios: the first is based on daily feeding (FR1); in the second, the feeding occurs once every two days (FR2); and in the third, the feeding happens once every three days (FR3). The results demonstrate that the biogas and methane yield increased by 34% and 37% between FR1 and FR3. The stability inside the reactor was maintained since specific loading energetic rate values did not exceed the recommended limit (0.4\u2009d\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). It was also possible to conclude that the AD technology was efficient to sanitise the pig slurry, with the count of Escherichia coli going from 1\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    \u2009colony-forming units (CFU)\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    to less than 100\u2009CFU\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    , meeting the legal requirements for agricultural valorisation. The total anaerobic mesophile plate counts were significantly ( p\u2009&lt;\u20090.1) reduced from feeding to digestate, and the plate counts of Clostridia were significantly ( p\u2009&lt;\u20090.05) increased, reflecting the changes in the composition of the microbiota. The increasing yield in bio-CH\n                    <jats:sub>4<\/jats:sub>\n                    in accordance with Clostridium counts suggests this genus as a positive microbiological key indicator of the AD performance.\n                  <\/jats:p>","DOI":"10.1177\/0734242x20972794","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T11:46:05Z","timestamp":1607600765000},"page":"947-955","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["A pig slurry feast\/famine feeding regime strategy to improve mesophilic anaerobic digestion efficiency and digestate hygienisation"],"prefix":"10.1177","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9341-092X","authenticated-orcid":false,"given":"I","family":"Silva","sequence":"first","affiliation":[{"name":"LEAF \u2013 Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisbon, Portugal"}]},{"given":"C","family":"Jorge","sequence":"additional","affiliation":[{"name":"Luke - Natural Resources Institute Finland, Helsinki, Finland"},{"name":"CEF - Forest Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisbon, Portugal"}]},{"given":"L","family":"Brito","sequence":"additional","affiliation":[{"name":"LEAF \u2013 Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisbon, Portugal"}]},{"given":"E","family":"Duarte","sequence":"additional","affiliation":[{"name":"LEAF \u2013 Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisbon, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2020,12,6]]},"reference":[{"key":"bibr1-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/J.ENG.2017.03.002"},{"key":"bibr2-0734242X20972794","volume-title":"Standard Methods for the Examination of Water and Wastewater","author":"American Public Health Association (APHA)","year":"2012","edition":"22"},{"key":"bibr3-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1279-5"},{"key":"bibr4-0734242X20972794","first-page":"47","volume":"13","author":"Boyd RD","year":"2002","journal-title":"Advances in Pork Production"},{"key":"bibr5-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.wasman.2018.10.041"},{"key":"bibr6-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-016-1760-8"},{"key":"bibr7-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1007\/s11367-015-1008-2"},{"key":"bibr8-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2019.122223"},{"key":"bibr9-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-017-0011-5"},{"key":"bibr10-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2017.02.036"},{"key":"bibr11-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.13044\/j.sdewes.d8.0320."},{"key":"bibr12-0734242X20972794","first-page":"170","author":"EU","year":"2019","journal-title":"Official Journal of the European Union"},{"key":"bibr13-0734242X20972794","volume-title":"An R Companion to Applied Regression","author":"Fox J","year":"2019","edition":"3"},{"key":"bibr14-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.694410"},{"key":"bibr15-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00540"},{"key":"bibr16-0734242X20972794","first-page":"210","volume":"2","author":"Kangle KM","year":"2011","journal-title":"Universal Journal of Environmental Research and Technology"},{"key":"bibr17-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2016.08.073"},{"key":"bibr18-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2016.05.046"},{"key":"bibr19-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1080\/09593330.2017.1420698"},{"key":"bibr20-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2019.121968"},{"key":"bibr21-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-016-0581-3"},{"key":"bibr22-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02320-15"},{"key":"bibr23-0734242X20972794","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2019.02.122"},{"key":"bibr24-0734242X20972794","unstructured":"R Core Team (2019) R: A language and environment for statistical computing. 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