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Characterization of the membranes was performed using atomic force microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Cytotoxicity assessment and direct contact assay were carried out for untreated and treated chitosan membranes using L929 fibroblast-like cells. Cell morphology and cell viability were assessed to evaluate the cell attachment and proliferation. Changes in terms of roughness, surface chemistry, and hydrophilicity\/hydrophobic balance of chitosan-modified membranes were observed. Regarding cell studies, the findings revealed that the extracts of all membranes do not induce cytotoxic effects. Moreover, the in vitro assays evidenced an improvement of the L929 adhesion and attachment when compared to untreated chitosan membranes. Overall, the data obtained clearly demonstrated that plasma treatments constitute an effective way of improving the biocompatibility of chitosan membranes towards to their use in biomedical applications. <\/jats:p>","DOI":"10.1177\/0885328210362924","type":"journal-article","created":{"date-parts":[[2010,5,29]],"date-time":"2010-05-29T02:59:38Z","timestamp":1275101978000},"page":"101-116","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":75,"title":["Cell Adhesion and Proliferation onto Chitosan-based Membranes Treated by Plasma Surface Modification"],"prefix":"10.1177","volume":"26","author":[{"given":"Sandra M.","family":"Luna","sequence":"first","affiliation":[{"name":"3B\u2019s Research Group \u2013 Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine \u2013 AvePark, Zona Industrial da Gandra \u2013 Caldas das Taipas 4806-909, Guimar\u00e3es, Portugal; IBB-Institute for Biotechnology and Bioengineering PT Government Associated Laboratory, Guimar\u00e3es, Portugal"}]},{"given":"Simone S.","family":"Silva","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group \u2013 Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine \u2013 AvePark, Zona Industrial da Gandra \u2013 Caldas das Taipas 4806-909, Guimar\u00e3es, Portugal IBB-Institute for Biotechnology and Bioengineering PT Government Associated Laboratory, Guimar\u00e3es, Portugal"}]},{"given":"Manuela E.","family":"Gomes","sequence":"additional","affiliation":[{"name":"3B\u2019s Research Group \u2013 Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine \u2013 AvePark, Zona Industrial da Gandra \u2013 Caldas das Taipas 4806-909, Guimar\u00e3es, Portugal; 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