{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:36:09Z","timestamp":1769164569462,"version":"3.49.0"},"reference-count":58,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T00:00:00Z","timestamp":1469145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Biomater Appl"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p> Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(\u025b-caprolactone)\/poly( m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride\/ N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in\u00a0vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2. <\/jats:p>","DOI":"10.1177\/0885328216660379","type":"journal-article","created":{"date-parts":[[2016,7,21]],"date-time":"2016-07-21T02:19:17Z","timestamp":1469067557000},"page":"743-754","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Enhanced osteogenesis on biofunctionalized poly(\u025b-caprolactone)\/poly(m-anthranilic acid) nanofibers"],"prefix":"10.1177","volume":"31","author":[{"given":"Zeliha","family":"Guler","sequence":"first","affiliation":[{"name":"Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Turkey"},{"name":"I3N\/Cenimat and Physics Department, Faculty of Science and Technology, Nova University of Lisbon, Caparica, Portugal"}]},{"given":"Jorge C","family":"Silva","sequence":"additional","affiliation":[{"name":"I3N\/Cenimat and Physics Department, Faculty of Science and Technology, Nova University of Lisbon, Caparica, Portugal"}]},{"given":"Abdulkadir S","family":"Sarac","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanoengineering, Istanbul Technical University, Istanbul, Turkey"},{"name":"Department of Chemistry, Istanbul Technical University, Istanbul, Turkey"},{"name":"Department of Polymer Science and Technology, Istanbul Technical University, Istanbul, Turkey"}]}],"member":"179","published-online":{"date-parts":[[2016,7,22]]},"reference":[{"key":"bibr1-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1042\/BA20050075"},{"key":"bibr2-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.08.004"},{"key":"bibr3-0885328216660379","doi-asserted-by":"publisher","DOI":"10.3144\/expresspolymlett.2016.11"},{"key":"bibr4-0885328216660379","doi-asserted-by":"publisher","DOI":"10.2174\/157016408785909622"},{"key":"bibr5-0885328216660379","doi-asserted-by":"crossref","first-page":"e0137017","DOI":"10.1371\/journal.pone.0137017","volume":"10","author":"Chang C-H","year":"2015","journal-title":"PloS One"},{"key":"bibr6-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19980315)68:4<411::AID-JCB2>3.0.CO;2-T"},{"key":"bibr7-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.04.021"},{"key":"bibr8-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.10.015"},{"key":"bibr9-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9879-y"},{"key":"bibr10-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1021\/bm900203w"},{"key":"bibr11-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1021\/la060754c"},{"key":"bibr12-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.01.036"},{"key":"bibr13-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30186"},{"key":"bibr14-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1021\/bm701267u"},{"key":"bibr15-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.02.004"},{"key":"bibr16-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0209"},{"key":"bibr17-0885328216660379","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.20043"},{"key":"bibr18-0885328216660379","doi-asserted-by":"crossref","unstructured":"Gazzarri M, Bartoli C, Mota C, et al. 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