{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:08:05Z","timestamp":1773292085670,"version":"3.50.1"},"reference-count":11,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":7754,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["slas-discovery.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["SLAS Discovery"],"published-print":{"date-parts":[[2001,2]]},"DOI":"10.1177\/108705710100600106","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T18:34:11Z","timestamp":1109270051000},"page":"39-46","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["FlashPlate Scintillation Proximity Assays for Characterization and Screening of DNA Polymerase, Primase, and Helicase Activities"],"prefix":"10.1016","volume":"6","author":[{"given":"David L.","family":"Earnshaw","sequence":"first","affiliation":[]},{"given":"Andrew J.","family":"Pope","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1177\/108705710100600106_bib1","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.1128\/AAC.36.12.2747","article-title":"Mode of antiviral action of Penciclovir in MRC-5 cells infected with herpes simplex virus type 1 (HSV-1), HSV-2, and varicella zoster virus","volume":"36","author":"Earnshaw","year":"1992","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1177\/108705710100600106_bib2","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1128\/AAC.42.11.2810","article-title":"DNA gyrase and topoisomerase IV are dual targets of clinafloxacin action in Streptococcus pneumoniae","volume":"42","author":"Pan","year":"1998","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1177\/108705710100600106_bib3","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1177\/108705719900400505","article-title":"Time-resolved fluorescence energy transfer DNA helicase assays for high throughput screening","volume":"4","author":"Earnshaw","year":"1999","journal-title":"J Biomol Screen"},{"key":"10.1177\/108705710100600106_bib4","series-title":"DNA Replication","first-page":"113","author":"Kornberg","year":"1992"},{"key":"10.1177\/108705710100600106_bib5","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1146\/annurev.bi.49.070180.002225","article-title":"Discontinuous DNA replication","volume":"49","author":"Ogawa","year":"1980","journal-title":"Annu Rev Biochem"},{"key":"10.1177\/108705710100600106_bib6","doi-asserted-by":"crossref","first-page":"6815","DOI":"10.1021\/bi00078a003","article-title":"Escherichia coli Rep helicase unwinds DNA by an active mechanism","volume":"32","author":"Amaratunga","year":"1993","journal-title":"Biochemistry"},{"key":"10.1177\/108705710100600106_bib7","doi-asserted-by":"crossref","first-page":"14306","DOI":"10.1021\/bi00251a044","article-title":"Single-turnover kinetics of helicase-catalysed DNA unwinding monitored continuously by fluorescence energy transfer","volume":"33","author":"Bjornson","year":"1994","journal-title":"Biochemistry"},{"key":"10.1177\/108705710100600106_bib8","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/abio.1995.0005","article-title":"Fluorescence recovery assay: a continuous assay for processive DNA polymerases applied specifically to DNA polymerase III holoenzyme","volume":"232","author":"Griep","year":"1995","journal-title":"Anal Biochem"},{"key":"10.1177\/108705710100600106_bib9","doi-asserted-by":"crossref","first-page":"16097","DOI":"10.1021\/bi00049a025","article-title":"Primer synthesis kinetics by Escherichia coli primase on single-stranded DNA templates","volume":"34","author":"Swart","year":"1995","journal-title":"Biochemistry"},{"key":"10.1177\/108705710100600106_bib10","doi-asserted-by":"crossref","first-page":"12970","DOI":"10.1016\/S0021-9258(18)31481-9","article-title":"Primase from Escherichia coli primes single-stranded templates in the absence of SSB or other auxiliary proteins: template requirements based on the bacteriophage G4 complementary strand origin and Okasaki fragment initiation sites","volume":"268","author":"Swart","year":"1993","journal-title":"J Biol Chem"},{"key":"10.1177\/108705710100600106_bib11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays","volume":"4","author":"Zhang","year":"1999","journal-title":"J Biomol Screen"}],"container-title":["SLAS Discovery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/108705710100600106","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S247263032200019X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S247263032200019X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/108705710100600106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T11:28:34Z","timestamp":1762860514000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S247263032200019X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":11,"aliases":["10.1089\/108705701750067024"],"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["S247263032200019X"],"URL":"https:\/\/doi.org\/10.1177\/108705710100600106","relation":{},"ISSN":["2472-5552"],"issn-type":[{"value":"2472-5552","type":"print"}],"subject":[],"published":{"date-parts":[[2001,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"FlashPlate Scintillation Proximity Assays for Characterization and Screening of DNA Polymerase, Primase, and Helicase Activities","name":"articletitle","label":"Article Title"},{"value":"SLAS Discovery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1177\/108705710100600106","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2001 Society for Laboratory Automation and Screening. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}