{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:20:37Z","timestamp":1777454437865,"version":"3.51.4"},"reference-count":54,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Breast Cancer\ufffd(Auckl)"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Male breast carcinoma (male BC) is an uncommon neoplasia without individualized strategies for diagnosis and therapeutics. Low overall survival (OS) rates have been reported, mostly associated with patients\u2019 advanced stage and older age. Intratumoral heterogeneity versus homogeneity of malignant epithelial cells seems to be an important factor to consider for the development of combination therapies with curative intention.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>In this preliminary study, we aim to provide valuable insight into the distinct clinicopathologic features of male BC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods:<\/jats:title>\n                    <jats:p>In a series of 40 male BC patients, we evaluated by immunohistochemistry androgen receptor; activating transcription factor 3 (ATF3); p16; cyclin D1; fatty acid synthase (FASN); fatty acid transport protein 1 (FATP1); \u03b21, \u03b23, \u03b24, and \u03b26 integrins; collagen I and collagen IV; and their interactions. Kaplan-Meier survival curves and log-rank tests were assessed for statistical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Homogeneous epithelial staining of p16, ATF3, \u03b26 integrin, FASN, and FATP1 was found to be significantly intercorrelated, and associated with high Ki67. These markers also stained tumor stromal fibroblasts. The prognostic analysis showed statistically significant associations of FASN with disease-free survival (DFS) and OS, as well as of ATF3 with OS and collagen IV with DFS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study highlights, as a novel finding, the relevance of FASN, ATF3, and collagen IV immunophenotypes, which may have innovative application in the clinical management of male BC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/11782234211002496","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T05:24:17Z","timestamp":1617859457000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Male Breast Cancer\u2014Immunohistochemical Patterns and Clinical Relevance of FASN, ATF3, and Collagen IV"],"prefix":"10.1177","volume":"15","author":[{"given":"Saudade","family":"Andr\u00e9","sequence":"first","affiliation":[{"name":"Department of Pathology, Portuguese Institute of Oncology of Lisbon, Lisbon, Portugal"}]},{"given":"Ant\u00f3nio E","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Pathology, Portuguese Institute of Oncology of Lisbon, Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7434-2383","authenticated-orcid":false,"given":"Giovani L","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Mathematics of Higher Technical Institute (Instituto Superior T\u00e9cnico), Faculty of Sciences (Faculdade de Ci\u00eancias), University of Lisbon, Lisbon, Portugal"},{"name":"Statistics and Applications Center of University of Lisbon (CEAUL), Lisbon, Portugal"}]},{"given":"Fernanda","family":"Silva","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School, NOVA University, Lisbon, Portugal"}]},{"given":"Jacinta","family":"Serpa","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School, NOVA University, Lisbon, Portugal"}]},{"given":"Ana","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"Department of Pathology, Portuguese Institute of Oncology of Lisbon, Lisbon, Portugal"},{"name":"CEDOC, NOVA Medical School, NOVA University, Lisbon, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"key":"bibr1-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1148\/rg.2018180013."},{"key":"bibr2-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2017.11.002."},{"key":"bibr3-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-17-0407."},{"key":"bibr4-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpdhp.2019.01.004"},{"key":"bibr5-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1159\/000477851."},{"key":"bibr6-11782234211002496","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2019.1841."},{"key":"bibr7-11782234211002496","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v5.i3.382."},{"key":"bibr8-11782234211002496","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.53."},{"key":"bibr9-11782234211002496","volume-title":"World Health Organization Classification of Tumors of the Breast. 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