{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T20:00:40Z","timestamp":1769976040092,"version":"3.49.0"},"reference-count":43,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Portuguese IBD Group (GEDII, Grupo de Estudo da Doen\u00e7a Inflamat\u00f3ria Intestinal)."}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therap Adv Gastroenterol"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> The loss of response to adalimumab (ADL) has been related to low serum concentrations at trough. Currently, most methods commercially available for the quantification of ADL are enzyme-linked immunosorbent assay (ELISA) based, with a turnaround time of approximately 8\u2004h, delaying the target dosage adjustment to the subsequent infusion. In this study, we aimed to evaluate the performance of the newly available rapid-test ADL quantification assay by comparing it with three established ELISA methods, using spiked samples and a set of clinical samples. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p> Spiked samples from control donors and 120 serum samples from inflammatory bowel disease (IBD) patients undergoing ADL therapy were quantified using lateral flow Quantum Blue<jats:sup>\u00ae<\/jats:sup> Adalimumab and, the ELISA formats from Immundiagnostik, R-Biopharm and an in-house assay. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> The rapid-test assay had intraclass correlation coefficients of 0.590, 0.864 and 0.761 when comparing with the Immundiagnostik, R-Biopharm and in-house assays, respectively. For the five therapeutic windows, the accuracy was high: ADL rapid test compared with the Immundiagnostik (58\u201388%); R-Biopharm, 68\u201389%; and in house, 60\u201388%; and kappa statistics revealed 0.492\u20130.602, 0.531\u20130.659 and 0.545\u20130.682, respectively. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p> The Quantum Blue<jats:sup>\u00ae<\/jats:sup> Adalimumab assay can replace the commonly used ELISA-based ADL quantification kits and it is a reliable alternative to these methods. This rapid-test assay enables the quantitative determination of ADL serum trough level in only 15\u2004min. The developed assay allows measurement of ADL over a wide range. Hence, it represents a valuable tool for the clinician to assess the ADL trough level. <\/jats:p><\/jats:sec>","DOI":"10.1177\/1756284819828238","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T04:30:51Z","timestamp":1551328251000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Accuracy of the new rapid test for monitoring adalimumab levels"],"prefix":"10.1177","volume":"12","author":[{"given":"C\u00e1tia","family":"Rocha","sequence":"first","affiliation":[{"name":"Department of Biomedicine, University of Porto, Porto, Portugal University of Lisbon, Faculty of Medicine, Instituto de S\u00e1ude Ambiental, Lisbon, Portugal"}]},{"given":"Joana","family":"Afonso","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, University of Porto, Porto, Portugal Centre for Drug Discovery and Innovative Medicines, University of Porto, Porto, Portugal"}]},{"given":"Paula","family":"Lago","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, Centro Hospitalar S\u00e3o Jo\u00e3o, Porto"}]},{"given":"Bruno","family":"Arroja","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, Hospital de Braga, Braga, Portugal"}]},{"given":"Ana I.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Gastroenterology Department, Hospital Garcia de Orta, Almada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9356-3272","authenticated-orcid":false,"given":"Claudia C.","family":"Dias","sequence":"additional","affiliation":[{"name":"Health Information and Decision Sciences Department, University of Porto, Porto, Portugal Centre for Health Technology and Services Research, University of Porto, Porto, Portugal"}]},{"given":"Fernando","family":"Magro","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Unit of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Gastroenterology Department, Centro Hospitalar S\u00e3o Jo\u00e3o, Porto, Portugal"},{"name":"MedInUP, Centre for Drug Discovery and Innovative Medicines, Porto, 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