{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:03:37Z","timestamp":1767924217785,"version":"3.49.0"},"reference-count":41,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese IBD Study Group"},{"DOI":"10.13039\/501100001871","name":"funda\u00e7\u00e3o para a ci\u00eancia e a tecnologia","doi-asserted-by":"publisher","award":["PDE\/BDE\/114583\/2016"],"award-info":[{"award-number":["PDE\/BDE\/114583\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therap Adv Gastroenterol"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Background and Aims:<\/jats:title><jats:p> Therapeutic drug monitoring (TDM) of infliximab (IFX) and anti-infliximab antibodies (ATIs) is essential for treatment optimisation in inflammatory bowel disease (IBD) patients. The aim of this study was to estimate and compare the agreement and accuracy between a new rapid test and three established enzyme-linked immunosorbent assays (ELISAs) to quantify ATIs levels, and to evaluate the impact of exogenous IFX on the performance of these assays. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p> We analysed 200 serum samples from 57 IBD outpatients in IFX induction or maintenance therapy at six IBD centres in Portugal. ATI levels were quantified using the rapid test Quantum Blue\u00ae (QB) Anti-Infliximab (B\u00fchlmann) and three established ELISAs: In-House, Theradiag (Lisa Tracker Anti-Infliximab), and Immundiagnostik (IDKmonitor Infliximab). ATIs were quantified in patients\u2019 serum samples and spiked samples with exogenous IFX, based on analytical and clinical cutoffs. Qualitative agreement and accuracy were estimated by Cohen\u2019s kappa ( k) with 95% confidence intervals. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> ATIs quantification with clinical cutoffs showed a slight agreement between QB rapid test and In-House [ k\u2009=\u20090.163 (0.051\u20130.276)] and Immundiagnostik [ k\u2009=\u20090.085 (0.000\u20130.177)]. Regarding IFX\/ATIs status, the QB rapid test showed a substantial agreement with Theradiag [ k\u2009=\u20090.808 (0.729\u20130.888)] and a fair agreement with In-House [ k\u2009=\u20090.343 (0.254\u20130.431)] and Immundiagnostik [ k\u2009=\u20090.217 (0.138\u20130.297)]. The QB rapid test could not detect ATI-positive levels in samples with exogenous IFX at 5\u2013300\u2009\u00b5g\/ml. Interference on ATIs detection was observed at exogenous IFX \u2a7e30\u2009\u00b5g\/ml for In-house and Immundiagnostik assays. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> QB rapid test is only suitable to detect ATI-positive levels in the absence of IFX. <\/jats:p><\/jats:sec>","DOI":"10.1177\/1756284820965790","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T06:51:29Z","timestamp":1605768689000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Rapid test detection of anti-infliximab antibodies: performance comparison with three different immunoassays"],"prefix":"10.1177","volume":"13","author":[{"given":"C\u00e1tia","family":"Rocha","sequence":"first","affiliation":[{"name":"Department of Biomedicine, Unit of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal"},{"name":"Faculty of Medicine, University of Lisbon, Lisbon, Portugal"},{"name":"Institute of Environmental Health, Faculty of Medicine, University of Lisbon, Lisbon, Portugal"}]},{"given":"Paula","family":"Lago","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Centro Hospitalar do Porto, Porto, Portugal"}]},{"given":"Samuel","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Centro Hospitalar Lisboa Norte, Hospital de Santa Maria, Lisbon, Portugal"}]},{"given":"Lu\u00eds","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Centro Hospitalar Lisboa Norte, Hospital de Santa Maria, Lisbon, Portugal"}]},{"given":"Francisco","family":"Portela","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Centro Hospitalar e Universit\u00e1rio de Coimbra, Coimbra, Portugal"}]},{"given":"Ana Isabel","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Garcia de Orta, Almada, Portugal"}]},{"given":"Marta","family":"Patita","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Garcia de Orta, Almada, Portugal"}]},{"given":"Bruno","family":"Arroja","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital de Braga, Braga, Portugal"}]},{"given":"Paula","family":"Ministro","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Centro Hospitalar Tondela-Viseu, Viseu, Portugal"}]},{"given":"Catarina","family":"Alves","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9356-3272","authenticated-orcid":false,"given":"Cl\u00e1udia Camila","family":"Dias","sequence":"additional","affiliation":[{"name":"Center for Health Technology and Services 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