{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:26:47Z","timestamp":1767652007742,"version":"3.48.0"},"reference-count":17,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Brazilian Ministry of Health (MoH), National Council for Scientific and Technological Development - CNPq, Brazil, and Brazilian National Program of Genomics and Precision Health \u2013 Genomas Brasil","award":["MS-SECTICS-Decit\/CNPq n\u00b0 16\/2023- INOVACRIAN\u00c7A"],"award-info":[{"award-number":["MS-SECTICS-Decit\/CNPq n\u00b0 16\/2023- INOVACRIAN\u00c7A"]}]},{"name":"Public Ministry of Labor Campinas, Brazil","award":["Research, Prevention, and Education of Occupationa"],"award-info":[{"award-number":["Research, Prevention, and Education of Occupationa"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ther Adv Med Oncol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Sarcoma diagnosis is challenging due to numerous subtypes with similar histopathological features and the high cost of fusion detection methods, particularly in middle-income countries.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>To implement a cost-effective custom-based nCounter approach previously validated for fusion analysis of suspected sarcoma in Brazil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design and methods:<\/jats:title>\n                    <jats:p>RNA isolated from 56 routine sarcomas, which were formalin-fixed and paraffin-embedded, was analyzed using a custom nCounter assay that detects 174 common sarcoma gene fusions. The results were compared to fluorescence in situ hybridization (FISH)\/next-generation sequencing (NGS) and clinicopathological data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The nCounter assay was conclusive in 98.2% of cases, identifying 25 gene fusions with 82.5% accuracy, 76.6% sensitivity, and 100% specificity compared to FISH\/NGS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Although it does not identify all sarcoma fusions, especially for rare subtypes, the present nCounter assay is a rapid, affordable, and accurate tool for sarcoma diagnosis in resource-limited settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17588359251318159","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T05:27:06Z","timestamp":1741930026000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing precision in sarcoma diagnosis: nCounter fusion panel implementation in a middle-income country"],"prefix":"10.1177","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9236-990X","authenticated-orcid":false,"given":"Fl\u00e1via Escremim","family":"de Paula","sequence":"first","affiliation":[{"name":"Molecular Diagnostic Laboratory, Barretos Cancer Hospital, Barretos, Brazil"}]},{"given":"Murilo","family":"Bonatelli","sequence":"additional","affiliation":[{"name":"Molecular Diagnostic Laboratory, Barretos Cancer Hospital, Barretos, Brazil"}]},{"given":"Monise Tadin","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Pathology Department, Barretos Cancer Hospital, Barretos, Brazil"}]},{"given":"Karla Em\u00edlia de S\u00e1","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Pediatric Department, Barretos Cancer Hospital, Barretos, Brazil"}]},{"given":"L\u00e9on C.","family":"van Kempen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"},{"name":"Department of Pathology, Antwerp University Hospital, University of Antwerp, Edegem, Belgium"}]},{"given":"Gustavo Ramos","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Molecular Diagnostic Laboratory, Barretos Cancer Hospital, Barretos, Brazil"},{"name":"Pathology Department, Barretos Cancer Hospital, Barretos, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-7940","authenticated-orcid":false,"given":"Rui Manuel","family":"Reis","sequence":"additional","affiliation":[{"name":"Molecular Diagnostic Laboratory, Barretos Cancer Hospital, Barretos, Brazil"},{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Rua Antenor Duarte Vilela, 1331, Barretos, Sao Paulo 14784-400, Brazil"},{"name":"Life and Health Sciences Research Institute, School of Medicine, University of Minho, Braga, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2021.109937"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201911131"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00991-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0748-7983(03)00006-4"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.32074\/1591-951X-213"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40348-022-00140-4"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0269-RA"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.path.2016.04.009"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.25013"},{"key":"e_1_3_4_11_2","article-title":"Improved gene fusion detection in childhood cancer diagnostics using RNA sequencing","volume":"6","author":"Hehir-Kwa JY","year":"2022","unstructured":"Hehir-Kwa JY, Koudijs MJ, Verwiel ETP, et al. Improved gene fusion detection in childhood cancer diagnostics using RNA sequencing. JCO Precis Oncol 2022; 6: e2000504.","journal-title":"JCO Precis Oncol"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22834"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.09.007"},{"key":"e_1_3_4_14_2","article-title":"STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies","volume":"351","author":"Bossuyt PM","year":"2015","unstructured":"Bossuyt PM, Reitsma JB, Bruns DE, et al. STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies. BMJ 2015; 351: h5527.","journal-title":"BMJ"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000521253"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdr610"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/17588359221144108"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.014"}],"container-title":["Therapeutic Advances in Medical Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17588359251318159","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/17588359251318159","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17588359251318159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:51:55Z","timestamp":1767649915000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/17588359251318159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":17,"alternative-id":["10.1177\/17588359251318159"],"URL":"https:\/\/doi.org\/10.1177\/17588359251318159","relation":{},"ISSN":["1758-8359","1758-8359"],"issn-type":[{"type":"print","value":"1758-8359"},{"type":"electronic","value":"1758-8359"}],"subject":[],"published":{"date-parts":[[2025,1]]},"article-number":"17588359251318159"}}