{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:29:58Z","timestamp":1767832198177,"version":"3.49.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod. Sci."],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1177\/1933719114530185","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T03:05:47Z","timestamp":1397099147000},"page":"1472-1482","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Molecular Cytogenetics of Human Single Pronucleated Zygotes"],"prefix":"10.1007","volume":"21","author":[{"given":"Ana Raquel","family":"Azevedo","sequence":"first","affiliation":[]},{"given":"Maria Jo\u00e3o","family":"Pinho","sequence":"additional","affiliation":[]},{"given":"Joaquina","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Ros\u00e1lia","family":"S\u00e1","sequence":"additional","affiliation":[]},{"given":"S\u00f3lveig","family":"Thorsteinsd\u00f3ttir","sequence":"additional","affiliation":[]},{"given":"Alberto","family":"Barros","sequence":"additional","affiliation":[]},{"given":"M\u00e1rio","family":"Sousa","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2014,12,30]]},"reference":[{"issue":"2","key":"21121472_CR1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1093\/oxfordjournals.humrep.a138026","volume":"8","author":"C Staessen","year":"1993","unstructured":"Staessen C, Janssenswillen C, Devroey P, Van Steirteghem AC. Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod. 1993;8(2): 221\u2013223.","journal-title":"Hum Reprod"},{"issue":"9","key":"21121472_CR2","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1093\/oxfordjournals.humrep.a138786","volume":"9","author":"ZP Nagy","year":"1994","unstructured":"Nagy ZP, Liu J, Joris H, Devroey P, Van Steirthghem A. Timecourse of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection. Hum Reprod. 1994;9(9):1743\u20131748.","journal-title":"Hum Reprod"},{"issue":"5","key":"21121472_CR3","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1093\/molehr\/2.5.299","volume":"2","author":"G Capmany","year":"1996","unstructured":"Capmany G, Taylor A, Braude PR, Bolton VN. The timing of pronuclear formation, DNA synthesis and cleavage in the human 1-cell embryo. Mol Hum Reprod. 1996;2(5):299\u2013306.","journal-title":"Mol Hum Reprod"},{"issue":"3","key":"21121472_CR4","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1093\/humrep\/12.3.532","volume":"12","author":"D Payne","year":"1997","unstructured":"Payne D, Flaherty SP, Barry MF, Matthews CD. Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod. 1997;12(3):532\u2013541.","journal-title":"Hum Reprod"},{"issue":"3","key":"21121472_CR5","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1080\/14647270600636269","volume":"9","author":"K Feenan","year":"2006","unstructured":"Feenan K, Herbert M. Can \u2018abnormally\u2019 fertilized zygotes give rise to viable embryos? Hum Fertil. 2006;9(3):157\u2013169.","journal-title":"Hum Fertil"},{"key":"21121472_CR6","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1093\/humrep\/4.suppl_1.99","volume":"4","author":"M Plachot","year":"1989","unstructured":"Plachot M, Mandelbaum J, Junca AM, de Grouchy J, Salat-Bar-oux J, Cohen J. Cytogenetic analysis and developmental capacity of normal and abnormal embryos after IVF. Hum Reprod. 1989;4(suppl):99\u2013103.","journal-title":"Hum Reprod"},{"issue":"4","key":"21121472_CR7","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1007\/BF01204942","volume":"10","author":"S Munn\u00e9","year":"1993","unstructured":"Munn\u00e9 S, Tang YX, Grifo J, Cohen J. Origin of single pronucleated human zygotes. J Assist Reprod Genet. 1993;10(4):276\u2013279.","journal-title":"J Assist Reprod Genet"},{"issue":"10","key":"21121472_CR8","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.1093\/oxfordjournals.humrep.a135757","volume":"10","author":"SP Flaherty","year":"1995","unstructured":"Flaherty SP, Payne D, Swann N, Matthews CD. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod. 1995;10(10):2623\u20132629.","journal-title":"Hum Reprod"},{"issue":"1","key":"21121472_CR9","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1093\/humrep\/10.1.132","volume":"10","author":"KM Sultan","year":"1995","unstructured":"Sultan KM, Munn\u00e9 S, Palermo GD, Alikani M, Cohen J. Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection. Hum Reprod. 1995;10(1):132\u2013136.","journal-title":"Hum Reprod"},{"issue":"4","key":"21121472_CR10","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/BF02070150","volume":"13","author":"E Macas","year":"1996","unstructured":"Macas E, Imthurn B, Roselli M, Keller PJ. Chromosome analysis of single- and multipronucleated human zygotes proceeded after the intracytoplasmic sperm injection procedure. J Assist Reprod Genet. 1996;13(4)345\u2013350.","journal-title":"J Assist Reprod Genet"},{"issue":"2","key":"21121472_CR11","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1093\/humrep\/12.2.321","volume":"12","author":"C Staessen","year":"1997","unstructured":"Staessen C, Van Steirteghem AC. The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in-vitro fertilization. Hum Reprod. 1997;12(2):321\u2013327.","journal-title":"Hum Reprod"},{"issue":"6","key":"21121472_CR12","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1093\/humupd\/4.6.842","volume":"4","author":"S Munn\u00e9","year":"1998","unstructured":"Munn\u00e9 S, Cohen J. Chromosome abnormalities in human embryos. Hum Reprod Update. 1998;4(6):842\u2013855.","journal-title":"Hum Reprod Update"},{"issue":"3","key":"21121472_CR13","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1093\/oxfordjournals.humrep.a138060","volume":"8","author":"H Balakier","year":"1993","unstructured":"Balakier H, Squire J, Casper RF. Characterization of abnormal one pronuclear human oocytes by morphology, cytogenetics and in-situ hybridization. Hum Reprod. 1993;8(3):402\u2013408.","journal-title":"Hum Reprod"},{"issue":"1","key":"21121472_CR14","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/mrd.20933","volume":"76","author":"GW Van Der Heijden","year":"2009","unstructured":"Van Der Heijden GW, Van Den Berg IM, Baart EB, Derijck AAHA, Martinini E, DeBoer P. Parental origin of chromatin in human monopronuclear zygotes revealed by asymmetric histone methylation patterns, differs between IVF and ICSI. Mol Reprod Dev. 2009;76(1):101\u2013108.","journal-title":"Mol Reprod Dev"},{"issue":"3","key":"21121472_CR15","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1016\/j.fertnstert.2012.11.014","volume":"99","author":"S Mateo","year":"2013","unstructured":"Mateo S, Parriego M, Boada M, Vidal F, Coroleu B, Veiga A. In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection. Fertil Steril. 2013;99(3):897\u2013902.","journal-title":"Fertil Steril"},{"issue":"3","key":"21121472_CR16","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1095\/biolreprod52.3.653","volume":"52","author":"J Levron","year":"1995","unstructured":"Levron J, Munn\u00e9Willadsen S, Rosenwaks Z, Cohen J. Male and female genomes associated in a single pronucleus in human zygotes. Biol Reprod. 1995;52(3):653\u2013657.","journal-title":"Biol Reprod"},{"issue":"7","key":"21121472_CR17","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.1093\/humrep\/14.7.1869","volume":"14","author":"L Gras","year":"1999","unstructured":"Gras L, Trouson A. Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization. Hum Reprod. 1999;14(7):1869\u20131871.","journal-title":"Hum Reprod"},{"issue":"12","key":"21121472_CR18","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s10815-004-8758-2","volume":"21","author":"D Dasig","year":"2004","unstructured":"Dasig D, Lyon J, Behr B, Milki AA. Monozygotic twin birth after the transfer of a cleavage stage embryo resulting from a single pronucleated oocyte. J Assist Reprod Genet. 2004;21(12): 427\u2013429.","journal-title":"J Assist Reprod Genet"},{"issue":"5","key":"21121472_CR19","first-page":"1","volume":"7","author":"F Pinto","year":"2009","unstructured":"Pinto F, Oliveira C, Cardoso MF, Teixeira-da-Silva J, Silva J, Sousa M, Barros A. Impact of GnRH ovarian stimulation protocols on intracytoplasmic sperm injection outcomes. Reprod Biol Endocrinol. 2009;7(5):1\u201310.","journal-title":"Reprod Biol Endocrinol"},{"issue":"4","key":"21121472_CR20","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1016\/S0015-0282(16)57853-4","volume":"64","author":"J Tesarik","year":"1995","unstructured":"Tesarik J, Sousa M. Key elements of a highly efficient intracytoplasmic sperm injection technique: Ca2+ fluxes and oocyte cytoplasmic dislocation. Fertil Steril. 1995;64(4):770\u2013776.","journal-title":"Fertil Steril"},{"issue":"11","key":"21121472_CR21","doi-asserted-by":"publisher","first-page":"3169","DOI":"10.1093\/humrep\/13.11.3169","volume":"13","author":"M Vandervorst","year":"1998","unstructured":"Vandervorst M, Liebaers I, Sermon K, et al. Successful preimplantation genetic diagnosis is related to the number of available cumulus-oocyte complexes. Hum Reprod. 1998;13(11):3169\u20133176.","journal-title":"Hum Reprod"},{"issue":"6","key":"21121472_CR22","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1016\/S0015-0282(00)00518-5","volume":"73","author":"DK Gardner","year":"2000","unstructured":"Gardner DK, Lane M, Stevens J, Schlenker T, Schollcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril. 2000;73(6): 1155\u20131158.","journal-title":"Fertil Steril"},{"issue":"6","key":"21121472_CR23","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1159\/000129914","volume":"5","author":"AK Tarkowski","year":"1966","unstructured":"Tarkowski AK. An air-drying method for chromosome preparations from mouse eggs. Cytogenetics. 1966;5(6):394\u2013400.","journal-title":"Cytogenetics"},{"issue":"3","key":"21121472_CR24","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1093\/oxfordjournals.humrep.a138540","volume":"9","author":"E Coonen","year":"1994","unstructured":"Coonen E, Dumoulin JCM, Ramaekers FCS, Hopman AHN. Optimal preparation of preimplantation embryo interphase nuclei for analysis by fluorescence in-situ hybridization. Hum Reprod. 1994;9(3):533\u2013537.","journal-title":"Hum Reprod"},{"issue":"4","key":"21121472_CR25","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1093\/oxfordjournals.humrep.a138577","volume":"9","author":"JC Harper","year":"1994","unstructured":"Harper JC, Coonen E, Ramaekers FCS, et al. Identification of the sex of human preimplantation embryos in two hours using an improved spreading method and fluorescent in-situ hybridization (FISH) using directly labelled probes. Hum Reprod. 1994;9(4):721\u2013724.","journal-title":"Hum Reprod"},{"issue":"12","key":"21121472_CR26","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1002\/pd.503","volume":"22","author":"C Alves","year":"2002","unstructured":"Alves C, Sousa M, Silva J, Barros A. Preimplantation genetic diagnosis using FISH for carriers of Robertsonian translocations: the Portuguese experience. Prenat Diagn. 2002;22(12):1153\u20131162.","journal-title":"Prenat Diagn"},{"issue":"1","key":"21121472_CR27","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.ydbio.2005.01.025","volume":"280","author":"F Santos","year":"2005","unstructured":"Santos F, Peters AH, Otte AP, Reik W, Dean W. Dynamic chromatin modifications characterise the first cell cycle in mouse embryos. Dev Biol. 2005;280(1):225\u2013236.","journal-title":"Dev Biol"},{"issue":"1","key":"21121472_CR28","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/s004120050422","volume":"109","author":"EB Baart","year":"2000","unstructured":"Baart EB, de Rooij DG, Keegan KS, de Boer O. Distribution of Atr protein in primary spermatocytes of a mouse chromosomal mutant: a comparison of preparation techniques. Chromosoma. 2000;109(1):139\u2013147.","journal-title":"Chromosoma"},{"issue":"5","key":"21121472_CR29","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1101\/gad.960502","volume":"16","author":"RD Goldman","year":"2002","unstructured":"Goldman RD, Gruenbaum Y, Moir RD, Shumaker DK, Spann TP. Nuclear lamins: building blocks of nuclear architecture. Genes Dev. 2002;16(5):533\u2013547.","journal-title":"Genes Dev"},{"issue":"1","key":"21121472_CR30","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1007\/s00412-002-0182-8","volume":"111","author":"IG Cowell","year":"2002","unstructured":"Cowell IG, Aucott R, Mahadevaiah SK, et al. Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma. 2002;111(1):22\u201336.","journal-title":"Chromosoma"},{"issue":"12","key":"21121472_CR31","doi-asserted-by":"publisher","first-page":"2374","DOI":"10.1093\/oxfordjournals.humrep.a138455","volume":"9","author":"M Sousa","year":"1994","unstructured":"Sousa M, Tesarik J. Ultrastructural analysis of fertilization failure after intracytoplasmic sperm injection. Hum Reprod. 1994;9(12): 2374\u20132380.","journal-title":"Hum Reprod"}],"container-title":["Reproductive Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1177\/1933719114530185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1933719114530185","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1177\/1933719114530185\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1933719114530185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1177\/1933719114530185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,3]],"date-time":"2022-07-03T01:32:14Z","timestamp":1656811934000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1177\/1933719114530185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["21121472"],"URL":"https:\/\/doi.org\/10.1177\/1933719114530185","relation":{},"ISSN":["1933-7191","1933-7205"],"issn-type":[{"value":"1933-7191","type":"print"},{"value":"1933-7205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12]]},"assertion":[{"value":"30 December 2014","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}