{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T15:24:37Z","timestamp":1768836277965,"version":"3.49.0"},"reference-count":49,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2015-0093829"],"award-info":[{"award-number":["2015-0093829"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Tissue Eng"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p> Bone\/cartilage interfacial tissue engineering needs to satisfy the differential properties and architectures of the osteochondral region. Therefore, biphasic or multiphasic scaffolds that aim to mimic the gradient hierarchy are widely used. Here, we find that two differently structured (topographically) three-dimensional scaffolds, namely, \u201cdense\u201d and \u201cnanofibrous\u201d surfaces, show differential stimulation in osteo- and chondro-responses of cells. While the nanofibrous scaffolds accelerate the osteogenesis of mesenchymal stem cells, the dense scaffolds are better in preserving the phenotypes of chondrocytes. Two types of porous scaffolds, generated by a salt-leaching method combined with a phase-separation process using the poly(lactic acid) composition, had a similar level of porosity (~90%) and pore size (~150 \u03bcm). The major difference in the surface nanostructure led to substantial changes in the surface area and water hydrophilicity (nanofibrous \u226b dense); as a result, the nanofibrous scaffolds increased the cell-to-matrix adhesion of mesenchymal stem cells significantly while decreasing the cell-to-cell contracts. Importantly, the chondrocytes, when cultured on nanofibrous scaffolds, were prone to lose their phenotype, including reduced chondrogenic expressions (SOX-9, collagen type II, and Aggrecan) and glycosaminoglycan content, which was ascribed to the enhanced cell\u2013matrix adhesion with reduced cell\u2013cell contacts. On the contrary, the osteogenesis of mesenchymal stem cells was significantly accelerated by the improved cell-to-matrix adhesion, as evidenced in the enhanced osteogenic expressions (RUNX2, bone sialoprotein, and osteopontin) and cellular mineralization. Based on these findings, we consider that the dense scaffold is preferentially used for the chondral-part, whereas the nanofibrous structure is suitable for osteo-part, to provide an optimal biphasic matrix environment for osteochondral tissue engineering. <\/jats:p>","DOI":"10.1177\/2041731419826433","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T14:29:29Z","timestamp":1548944969000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Differential chondro- and osteo-stimulation in three-dimensional porous scaffolds with different topological surfaces provides a design strategy for biphasic osteochondral engineering"],"prefix":"10.1177","volume":"10","author":[{"given":"Chinmaya","family":"Mahapatra","sequence":"first","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea"}]},{"given":"Jung-Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea"}]},{"given":"Jung-Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, Republic of Korea"},{"name":"UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea"}]},{"given":"Guang-Zhen","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea"},{"name":"UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea"}]},{"given":"Jonathan C","family":"Knowles","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea"},{"name":"Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-6100","authenticated-orcid":false,"given":"Hae-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea"},{"name":"Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea"},{"name":"UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of 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