{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:44:40Z","timestamp":1790203480739,"version":"4.1.0"},"reference-count":43,"publisher":"American Society of Hematology","issue":"9","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Because tumor progression is angiogenesis-dependent, angiogenesis density was investigated by immunohistochemistry and computed image analysis in bone marrow (BM) biopsies of 45 newly diagnosed patients with Binet stage A B-cell chronic lymphocytic leukemia (BCLL) and correlated to upstaging and progression-free survival during a 40-month follow-up period. Their microvessel areas and counts were significantly higher than those of patients with anemia due to iron or vitamin B12deficiencies. A cutoff value of 0.90 mm2\u2009\u00d7\u200910\u22122 or greater of the microvessel area identified patients with earlier upstaging and shorter progression-free survival. When the cutoff was applied to the Rai subclassification, both Rai 0 and Rai I-II patients who upstaged and shortened the progression-free survival were classified correctly. Information of this type was not given by the microvessel counts. The cutoff did not correlate with other predictors representative of tumor mass or disease progression. The microvessel area correlated with the expression of angiogenic vascular endothelial growth factor (VEGF) by tumor tissue, and serum levels of VEGF were found to be of prognostic value. A causal relationship between risk of progression and BM angiogenesis in BCLL is suggested. A risk stratification inside Rai is proposed. The prognostic usefulness of BM angiogenesis in patients with BCLL is envisaged.<\/jats:p>","DOI":"10.1182\/blood-2002-01-0084","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T19:03:08Z","timestamp":1034794988000},"page":"3344-3351","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":99,"title":["Prognostic value of enhanced bone marrow angiogenesis in early B-cell chronic lymphocytic leukemia"],"prefix":"10.1182","volume":"100","author":[{"given":"Stefano","family":"Molica","sequence":"first","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Vacca","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Ribatti","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cuneo","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Cavazzini","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Levato","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Vitelli","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Tucci","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo M.","family":"Roccaro","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Dammacco","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology\/Oncology, and Anatomy and Histopathology, Azienda Ospedaliera \u201cPugliese-Ciaccio,\u201d Catanzaro, Italy; Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, and Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy; Institute of Hematology, University of Ferrara, Ferrara, Italy; and Clinical Pathology Service, Institute \u201cRegina Elena\u201d IRCCS, Rome, 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