{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:53:24Z","timestamp":1771005204641,"version":"3.50.1"},"reference-count":35,"publisher":"American Society of Hematology","issue":"9","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immunosuppressive drugs, cyclosporine A (CsA), tacrolimus, or sirolimus, were analyzed as single agents and in combination with anti-CD40L monoclonal antibody (mAb) for their effects on alloengraftment in mice conditioned with minimal total body irradiation (TBI). Whereas anti-CD40L mAb facilitated chimerism, neither sirolimus nor CsA resulted in substantial alloengraftment. However, sirolimus was synergistic with anti-CD40L mAb for inducing donor chimerism. Contrary to expectations, CsA, a T-cell receptor (TCR) signaling inhibitor, did not abrogate anti-CD40L mAb-facilitated engraftment but rather increased engraftment in anti-CD40L mAb-treated mice. Although tacrolimus alone or with anti-CD40L mAb resulted in similar levels of donor chimerism, donor T-cell reconstitution was very low in tacrolimus-treated mice. At 1 week after transplantation, CsA decreased thymic numbers more profoundly than sirolimus or tacrolimus in anti-CD40L mAb-treated recipients. In contrast, only sirolimus resulted in a decrease in host splenic T-cell numbers in anti-CD40L mAb-treated recipients. Importantly, sirolimus and anti-CD40L mAb induced profound donor tolerance with 100% acceptance of donor skin grafts placed early after bone marrow transplantation (BMT). In contrast, anti-CD40L mAb alone or in combination with CsA resulted in 12% or less donor skin graft acceptance early (1 month) and 60% or less later (3 months) after BMT. These data have clinical relevance and indicate that immunosuppressive pharmacologic agents enhance anti-CD40L mAb-facilitated alloengraftment and tolerance induction under nonmyeloablative conditioning.<\/jats:p>","DOI":"10.1182\/blood-2002-03-0872","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T23:03:08Z","timestamp":1034809388000},"page":"3400-3407","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":77,"title":["Combined effects of calcineurin inhibitors or sirolimus with anti-CD40L mAb on alloengraftment under nonmyeloablative conditions"],"prefix":"10.1182","volume":"100","author":[{"given":"Patricia A.","family":"Taylor","sequence":"first","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Lees","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica M.","family":"Wilson","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Ehrhardt","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew T.","family":"Campbell","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randolph J.","family":"Noelle","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone Marrow Transplantation, Minneapolis, MN; and the Department of Microbiology, Dartmouth Medical College, Hanover, NH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce R.","family":"Blazar","sequence":"additional","affiliation":[{"name":"From the University of Minnesota Cancer Center and Department of Pediatrics, 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