{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:46:46Z","timestamp":1761540406328},"reference-count":56,"publisher":"American Society of Hematology","issue":"11","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Shear-induced platelet aggregation (SIPA) involves the sequential interaction of von Willebrand factor (VWF) with both glycoprotein Ib (GPIb) and \u03b1IIb\u03b23 receptors. Type 2B recombinant VWF (2B-rVWF), characterized by an increased affinity for GPIb, induces strong SIPA at a high shear rate (4000 s\u20131). Despite the increased affinity of 2B-rVWF for GPIb, patients with type 2B von Willebrand disease have a paradoxical bleeding disorder, which is not well understood. The purpose of this study was to determine if SIPA induced by 2B-rVWF was associated with \u03b1IIb\u03b23-dependent platelet activation. To this end, we have addressed the influence of 2B-rVWF (Val553Met substitution) on SIPA-dependent variations of tyrosine protein phosphorylation (P-Tyr) and the effect of \u03b1IIb\u03b23 blockers. At a high shear rate, 2B-rVWF induced a strong SIPA, as shown by a 92.7% \u00b1 0.4% disappearance of single platelets (DSP) after 4.5 minutes. In these conditions, increased P-Tyr of proteins migrating at positions 64 kd, 72 kd, and 125 kd were observed. The band at 125 kd was identified as pp125FAK using anti\u2013phospho-FAK antibody. This effect, which required a high level of SIPA (&amp;gt; 70% DSP), was observed at 4000 s\u20131 but not at 200 s\u20131. Monoclonal antibodies (MoAbs) 6D1 (anti-GPIb) and 328 (anti-VWF A1 domain), completely abolished SIPA and p125FAK phosphorylation mediated by 2B-rVWF. In contrast, neither RGDS peptide nor MoAb 7E3, both known to block \u03b1IIb\u03b23 engagement, had any effect on SIPA and pp125FAK. The size of aggregates formed at a high shear rate in the presence of 2B-rVWF was decreased by genistein, demonstrating the biologic relevance of pp125FAK. These findings provide a unique mechanism whereby the enhanced interaction of 2B-rVWF with GPIb, without engagement of \u03b1IIb\u03b23, is sufficient to induce SIPA but does not lead to stable thrombus formation.<\/jats:p>","DOI":"10.1182\/blood-2002-06-1879","type":"journal-article","created":{"date-parts":[[2003,5,20]],"date-time":"2003-05-20T01:21:48Z","timestamp":1053393708000},"page":"4363-4371","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":7,"title":["Activation of pp125FAK by type 2B recombinant von Willebrand factor binding to platelet GPIb at a high shear rate occurs independently of \u03b1IIb\u03b23 engagement"],"prefix":"10.1182","volume":"101","author":[{"given":"Me\u0301dina","family":"Mekrache","sequence":"first","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christilla","family":"Bachelot-Loza","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Ajzenberg","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelhafid","family":"Saci","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulette","family":"Legendre","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Baruch","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U143, Le Kremlin-Bicetre, France; and INSERM U428, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021221364132800_REF1","doi-asserted-by":"crossref","unstructured":"Savage B, Saldivar E, Ruggeri ZM. 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