{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:19:48Z","timestamp":1790867988658,"version":"4.1.0"},"reference-count":25,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Hemophilia A is a life-threatening, hemorrhagic, X-linked recessive disorder resulting in deficient factor VIII (FVIII) activity. After the infusion of therapeutic FVIII, 25% of patients develop anti-FVIII antibodies that inhibit FVIII procoagulant activity, thus precluding further administration of FVIII. Here we report a novel approach aimed at neutralizing the activity of FVIII inhibitors by peptide epitope surrogates. To illustrate our concept, we chose the human anti-FVIII monoclonal antibody, Bo2C11, as a representative of anti-FVIII antibodies and a phage-displayed peptide library approach to obtain surrogate peptides. We selected a series of constrained dodecapeptides with the core sequence W-NR, which specifically interacts with the combining site of Bo2C11. The peptides mimic the epitope recognized by Bo2C11 and are able to inhibit specifically and in a dose-dependent manner the binding of Bo2C11 to FVIII. Peptide 107, in particular, neutralized the activity of Bo2C11 in vitro and restored normal hemostasis in hemophilic mice. Thus, the use of peptide decoys may be a promising new approach for the neutralization of pathologic antibodies.<\/jats:p>","DOI":"10.1182\/blood-2002-06-1886","type":"journal-article","created":{"date-parts":[[2003,5,6]],"date-time":"2003-05-06T00:14:56Z","timestamp":1052180096000},"page":"949-952","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":47,"title":["Peptide decoys selected by phage display block in vitro and in vivo activity of a human anti-FVIII inhibitor"],"prefix":"10.1182","volume":"102","author":[{"given":"Sylvie","family":"Villard","sequence":"first","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u0301bastien","family":"Lacroix-Desmazes","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kieber-Emmons","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Piquer","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Grailly","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdellah","family":"Benhida","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srini V.","family":"Kaveri","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Saint-Remy","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Granier","sequence":"additional","affiliation":[{"name":"From the Unite\u0301 Mixte de Recherche 5094 of the Centre National de la Recherche Scientifique Institut National de la Recherche Me\u0301dicale, Faculte\u0301 de Pharmacie, Montpellier; Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U430, Institut des Cordeliers, Paris, France; the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; and the Center for Molecular and Vascular Biology, University of Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021221272173300_REF1","doi-asserted-by":"crossref","unstructured":"Vehar GA, Keyt B, Eaton D, et al. 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Two classes of germline genes both derived from the V(H)1 family direct the formation of human antibodies that recognize distinct antigenic sites in the C2 domain of factor VIII. Blood. 2002;99: 2828-2834.","DOI":"10.1182\/blood.V99.8.2828"},{"key":"2020021221272173300_REF24","doi-asserted-by":"crossref","unstructured":"Healey JF, Lubin IM, Nakai H, et al. Residues 484-508 contain a major determinant of the inhibitory epitope in the A2 domain of human factor VIII. J Biol Chem.1995;270: 14505-14509.","DOI":"10.1074\/jbc.270.24.14505"},{"key":"2020021221272173300_REF25","doi-asserted-by":"crossref","unstructured":"Folgori A, Tafi R, Meola A, et al. A general strategy to identify mimotopes of pathological antigens using only random peptide libraries and human sera. EMBO J.1994;13: 2236-2243.","DOI":"10.1002\/j.1460-2075.1994.tb06501.x"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/102\/3\/949\/1689334\/h81503000949.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/102\/3\/949\/1689334\/h81503000949.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T02:28:45Z","timestamp":1581560925000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/102\/3\/949\/17312\/Peptide-decoys-selected-by-phage-display-block-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8,1]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,8,1]]}},"URL":"https:\/\/doi.org\/10.1182\/blood-2002-06-1886","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8,1]]}}}