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The present studies confirm that candidate HSCs in human cord blood and BM lack constitutive expression of Fas, but demonstrate that Fas expression on CD34+ progenitor and stem cells is correlated to their cell cycle and activation status. With the use of recently developed in vitro conditions promoting HSC self-renewing divisions, Fas was up-regulated on virtually all HSCs capable of multilineage reconstituting nonobese diabetic\/severe combined immunodeficiency (NOD-SCID) mice in vivo, as well as on long-term culture-initiating cells (LTC-ICs). Similarly, in vivo cycling of NOD-SCID repopulating cells upon transplantation, resulted in up-regulation of Fas expression. However, repopulating HSCs expressing high levels of Fas remained highly resistant to Fas-mediated suppression, and HSC function was compromised only upon coactivation with tumor necrosis factor. Thus, reconstituting human HSCs up-regulate Fas expression upon active cycling, demonstrating that HSCs could be targets for Fas-mediated BM suppression. (Blood. 2003;102: 118-126)<\/jats:p>","DOI":"10.1182\/blood-2002-07-2286","type":"journal-article","created":{"date-parts":[[2003,3,18]],"date-time":"2003-03-18T16:13:45Z","timestamp":1048004025000},"page":"118-126","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":21,"title":["Human reconstituting hematopoietic stem cells up-regulate Fas expression upon active cell cycling but remain resistant to Fas-induced suppression"],"prefix":"10.1182","volume":"102","author":[{"given":"Ingunn","family":"Dybedal","sequence":"first","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Yang","sequence":"additional","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bryder","sequence":"additional","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingbritt","family":"Aastrand-Grundstrom","sequence":"additional","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Leandersson","sequence":"additional","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten Eirik W.","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"From the Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University Hospital, Lund, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021218073764800_REF1","doi-asserted-by":"crossref","unstructured":"Reya T, Morrison SJ, Clarke MF, Weissman IL. 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