{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:02:18Z","timestamp":1791453738999,"version":"4.3.3"},"reference-count":37,"publisher":"American Society of Hematology","issue":"5","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:p>The chemokine receptors (CCRs) CCR4 and CCR10, and the cutaneous lymphocyte antigen (CLA), have each been proposed as critical mediators of skin-specific TH lymphocyte homing in mice and humans. CLA initiates skin homing by mediating E-selectin\u2013dependent tethering and rolling within cutaneous venules, but the specific roles of CCR4 and CCR10 are unclear. We have generated an antihuman CCR10 monoclonal antibody (mAb; 1B5) to illuminate the individual contributions of these molecules. This mAb allows us to compare CCR10, CCR4, and CLA expression within human THpopulations. The mAb 1B5 recognizes functional CCR10 expression, as chemotactic responsiveness to cutaneous T-cell\u2013attracting chemokine (CTACK)\/CCL27 (a CCR10 ligand) parallels the staining of TH subsets. We find CCR10 expressed by only a minority (approximately 30%) of blood-borne, skin-homing (CLA+\/CCR4+) TH cells. However, essentially all members of the relatively small \u201ceffector\u201d (CLA+\/CCR4+\/CD27\u2212\/CCR7\u2212) skin-homing TH population express CCR10. Most skin-infiltrating lymphocytes in allergic delayed-type hypersensitivity (DTH) and bacterial chancroid skin lesions express both CCR4 and CLA, but only about 10% express CCR10. This suggests for the 2 models of TH skin homing studied here that CCR10+ TH cells have no advantage over other CLA+\/CCR4+ TH cells in homing to cutaneous sites. We conclude that the skin-homing THcompartment is itself divided into distinct subpopulations, the smaller of which expresses both CCR4 and CCR10, and the larger of which expresses only CCR4. Thus, CCR10 is unlikely to be necessary for cutaneous homing of TH cells in the models studied here. CCR10 may instead play a role in the movement of specialized \u201ceffector\u201d cutaneous TH cells to and\/or within epidermal microenvironments.<\/jats:p>","DOI":"10.1182\/blood-2002-07-2348","type":"journal-article","created":{"date-parts":[[2003,2,12]],"date-time":"2003-02-12T19:59:36Z","timestamp":1045079976000},"page":"1677-1682","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":168,"title":["CCR4 versus CCR10 in human cutaneous TH lymphocyte trafficking"],"prefix":"10.1182","volume":"101","author":[{"given":"Dulce","family":"Soler","sequence":"first","affiliation":[{"name":"From Millennium Pharmaceuticals, Inflammation Department, Cambridge, MA; the Department of Medicine, Indiana University School of Medicine, Indianapolis, IN; the Departments of Microbiology and Immunology, and Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN; the Department of Pathology, Harvard Medical School, Boston, MA; and the Joint Program in Transfusion Medicine, Children's Hospital, Boston, MA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tricia L.","family":"Humphreys","sequence":"additional","affiliation":[{"name":"From Millennium Pharmaceuticals, Inflammation Department, Cambridge, MA; the Department of Medicine, Indiana University School of Medicine, Indianapolis, IN; the Departments of Microbiology and Immunology, and Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN; the Department of Pathology, Harvard Medical School, Boston, MA; and the Joint Program in Transfusion Medicine, Children's Hospital, Boston, MA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley M.","family":"Spinola","sequence":"additional","affiliation":[{"name":"From Millennium Pharmaceuticals, Inflammation Department, Cambridge, MA; the Department of Medicine, Indiana University School of Medicine, Indianapolis, IN; the Departments of Microbiology and Immunology, and Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN; the Department of Pathology, Harvard Medical School, Boston, MA; and the Joint Program in Transfusion Medicine, Children's Hospital, Boston, MA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Campbell","sequence":"additional","affiliation":[{"name":"From Millennium Pharmaceuticals, Inflammation Department, Cambridge, MA; the Department of Medicine, Indiana University School of Medicine, Indianapolis, IN; the Departments of Microbiology and Immunology, and Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN; the Department of Pathology, Harvard Medical School, Boston, MA; and the Joint Program in Transfusion Medicine, Children's Hospital, Boston, MA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2019111900301615500_B1","doi-asserted-by":"crossref","first-page":"4227","DOI":"10.4049\/jimmunol.161.8.4227","article-title":"The memory B cell subset responsible for the secretory IgA response and protective humoral immunity to rotavirus expresses the intestinal homing receptor, alpha4beta7.","volume":"161","author":"Williams","year":"1998","journal-title":"J Immunol."},{"key":"2019111900301615500_B2","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1172\/JCI119633","article-title":"Expression of mucosal homing receptor alpha4beta7 by circulating CD4+ cells with memory for intestinal rotavirus.","volume":"100","author":"Rott","year":"1997","journal-title":"J Clin Invest."},{"key":"2019111900301615500_B3","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1172\/JCI10927","article-title":"alpha(4)beta(7) independent pathway for CD8(+) T cell-mediated intestinal immunity to rotavirus.","volume":"106","author":"Kuklin","year":"2000","journal-title":"J Clin Invest."},{"key":"2019111900301615500_B4","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/0952-7915(93)90063-X","article-title":"Homing of naive, memory and effector lymphocytes.","volume":"5","author":"Mackay","year":"1993","journal-title":"Curr Opin Immunol."},{"key":"2019111900301615500_B5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF02935627","article-title":"Skin-homing T cells in human cutaneous allergic inflammation.","volume":"14","author":"Santamaria Babi","year":"1995","journal-title":"Immunol Res."},{"key":"2019111900301615500_B6","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1084\/jem.181.5.1935","article-title":"Circulating allergen-reactive T cells from patients with atopic dermatitis and allergic contact dermatitis express the skin-selective homing receptor, the cutaneous lymphocyte-associated antigen.","volume":"181","author":"Santamaria Babi","year":"1995","journal-title":"J Exp Med."},{"key":"2019111900301615500_B7","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1084\/jem.174.6.1461","article-title":"The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1.","volume":"174","author":"Berg","year":"1991","journal-title":"J Exp Med."},{"key":"2019111900301615500_B8","first-page":"1053","article-title":"A unique phenotype of skin-associated lymphocytes in humans. 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