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The phenotyping of rapidly recruited inflammatory leukocytes with 7\/4 and Gr-1 monoclonal antibodies (mAbs) identifies 2 distinct populations, which we characterize as murine monocytes and neutrophils. Monocytes migrate in the first 2 hours of inflammation making use of \u03b14\u03b21 but not of Mac-1 or lymphocyte function-associated antigen-1 (LFA-1) integrins. Early migration is dependent on MCP-1, but neither MCP-1 release nor monocyte recruitment is affected by the reduced neutrophil migration seen in LFA-1-\/- mice. Endogenous peritoneal macrophages and mesothelial cells lining the peritoneum contain MCP-1, which is released following thioglycollate stimulation. The murine monocyte therefore responds rapidly to chemokines produced in situ by tissue cells at the site of inflammation with no requirement for prior influx of neutrophils. (Blood. 2003;102:328-335)<\/jats:p>","DOI":"10.1182\/blood-2002-10-3228","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T02:12:56Z","timestamp":1047348776000},"page":"328-335","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":240,"title":["Rapid recruitment of inflammatory monocytes is independent of neutrophil migration"],"prefix":"10.1182","volume":"102","author":[{"given":"Robert B.","family":"Henderson","sequence":"first","affiliation":[{"name":"From the Leukocyte Adhesion Laboratory, Cancer Research United Kingdom London Research Institute, Lincoln's Inn Fields Laboratories, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josie A. 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