{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:13:07Z","timestamp":1787314387800,"version":"build-2736575974"},"reference-count":18,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Using loss of heterozygosity (LOH) and X-chromosome inactivation, we compared peripheral blood (PB) plasma with bone marrow (BM) cells in detecting genomic abnormalities in patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). We detected LOH in the PB plasma of all 45 patients who had cytogenetically documented chromosomal abnormalities (5q-, 7-, +8, 17-, or 20-). BM cells from the same patients showed LOH in 89% of patients with MDS and 70% of patients with AML. Posttherapy samples from 16 of these patients demonstrated complete concordance between LOH and cytogenetics in detecting residual disease in 15 samples. Of the 16 samples, 4 showed LOH in plasma with normal BM morphology. Using X-chromosome inactivation, clonality was detectable in 19 (73%) of 26 BM samples, whereas all PB plasma samples showed clonality. These data support the conclusion that PB plasma is enriched by tumor-specific DNA and can replace BM cells for studying genomic abnormalities. (Blood. 2004;103:2799-2801)<\/jats:p>","DOI":"10.1182\/blood-2003-06-1840","type":"journal-article","created":{"date-parts":[[2003,10,28]],"date-time":"2003-10-28T02:45:28Z","timestamp":1067309128000},"page":"2799-2801","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":45,"title":["Relative increase in leukemia-specific DNA in peripheral blood plasma from patients with acute myeloid leukemia and myelodysplasia"],"prefix":"10.1182","volume":"103","author":[{"given":"Anna","family":"Rogers","sequence":"first","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngson","family":"Joe","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taghi","family":"Manshouri","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Dey","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman","family":"Jilani","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Giles","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elihu","family":"Estey","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil","family":"Freireich","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Keating","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagop","family":"Kantarjian","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher","family":"Albitar","sequence":"additional","affiliation":[{"name":"From the Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, and the Nichols Institute, Quest Diagnostics, San Juan Capistrano, CA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021217543954700_REF1","doi-asserted-by":"crossref","unstructured":"Albitar M, Manshouri T, Shen Y, et al. Myelodysplastic syndrome is not merely \u201cpreleukemia.\u201d Blood. 2002;100: 791-798.","DOI":"10.1182\/blood.V100.3.791"},{"key":"2020021217543954700_REF2","unstructured":"Chen X, Bonnefoi H, Diebold-Berger S, et al. Detecting tumor-related alterations in plasma or serum DNA of patients diagnosed with breast cancer. Clin Cancer Res. 1999;5: 2297-2303."},{"key":"2020021217543954700_REF3","doi-asserted-by":"crossref","unstructured":"Nakayama T, Taback B, Nguyen DH, et al. Clinical significance of circulating DNA microsatellite markers in plasma of melanoma patients. Ann N Y Acad Sci. 2000;906: 87-98.","DOI":"10.1111\/j.1749-6632.2000.tb06596.x"},{"key":"2020021217543954700_REF4","doi-asserted-by":"crossref","unstructured":"Nawroz H, Koch W, Anker P, Stroun M, Sidransky D. Microsatellite alterations in serum DNA of head and neck cancer patients. Nat Med. 1996;2: 1035-1037.","DOI":"10.1038\/nm0996-1035"},{"key":"2020021217543954700_REF5","doi-asserted-by":"crossref","unstructured":"Sanchez-Cespedes M, Monzo M, Rosell R, et al. Detection of chromosome 3p alterations in serum DNA of non-small-cell lung cancer patients. Ann Oncol. 1998;9: 113-116.","DOI":"10.1023\/A:1008230331221"},{"key":"2020021217543954700_REF6","doi-asserted-by":"crossref","unstructured":"Vasioukhin V, Anker P, Maurice P, Lyautey J, Lederrey C, Stroun M. Point mutations of the N-ras gene in the blood plasma DNA of patients with myelodysplastic syndrome or acute myelogenous leukaemia. Br J Haematol. 1994;86: 774-779.","DOI":"10.1111\/j.1365-2141.1994.tb04828.x"},{"key":"2020021217543954700_REF7","doi-asserted-by":"crossref","unstructured":"Frickhofen N, Muller E, Sandherr M, et al. Rearranged Ig heavy chain DNA is detectable in cell-free blood samples of patients with B-cell neoplasia. Blood. 1997;90: 4953-4960.","DOI":"10.1182\/blood.V90.12.4953"},{"key":"2020021217543954700_REF8","doi-asserted-by":"crossref","unstructured":"Dasi F, Lledo S, Garcia-Granero E, et al. Real-time quantification in plasma of human telomerase reverse transcriptase (hTERT) mRNA: a simple blood test to monitor disease in cancer patients. Lab Invest. 2001;81: 767-769.","DOI":"10.1038\/labinvest.3780285"},{"key":"2020021217543954700_REF9","doi-asserted-by":"crossref","unstructured":"Jilani I, Estey E, Manshouri T, et al. Better detection of FLT3 internal tandem duplication using peripheral blood plasma DNA. Leukemia. 2003;17: 114-119.","DOI":"10.1038\/sj.leu.2402743"},{"key":"2020021217543954700_REF10","unstructured":"Feng BZ, Lei LJ, Han JZ. Cytogenetic studies on 334-myelodysplastic syndrome (MDS), aplastic anemia (AA) and other hematological diseases. Chung Hua Nei Ko Tsa Chih. 1994;33: 754."},{"key":"2020021217543954700_REF11","unstructured":"Ghaddar HM SS, Pierce S, Estey EH. Cytogenetic evolution following the transformation of myelodysplastic syndrome to acute myelogenous leukemia: implications on the overlap between the two diseases. Leukemia. 1994;8: 1649-1653."},{"key":"2020021217543954700_REF12","doi-asserted-by":"crossref","unstructured":"Geddes AA, Bowen DT, Jacobs A. Clonal karyotype abnormalities and clinical progress in the myelodysplastic syndrome. Br J Haematol. 1990;76: 194-202.","DOI":"10.1111\/j.1365-2141.1990.tb07871.x"},{"key":"2020021217543954700_REF13","doi-asserted-by":"crossref","unstructured":"Swansbury GJ. The proportion of clonal divisions varies in different hematologic malignancies. The United Kingdom Cancer Cytogenetics Groups (UKCCG). Cancer Genet Cytogenet. 1998;104: 139-145.","DOI":"10.1016\/S0165-4608(97)00477-9"},{"key":"2020021217543954700_REF14","doi-asserted-by":"crossref","unstructured":"Asimakopoulos FA, White NJ, Nacheva E, Green AR. Molecular analysis of chromosome 20q deletions associated with myeloproliferative disorders and myelodysplastic syndromes. Blood. 1994;84: 3086-3094.","DOI":"10.1182\/blood.V84.9.3086.3086"},{"key":"2020021217543954700_REF15","doi-asserted-by":"crossref","unstructured":"Fairman J, Wang RY, Liang H, et al. Translocations and deletions of 5q13.1 in myelodysplasia and acute myelogenous leukemia: evidence for a novel critical locus. Blood. 1996;88: 2259-2266.","DOI":"10.1182\/blood.V88.6.2259.bloodjournal8862259"},{"key":"2020021217543954700_REF16","unstructured":"Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome inactivation. Am J Hum Genet. 1992;51: 1229-1239."},{"key":"2020021217543954700_REF17","unstructured":"Gilliland DG, Blanchard KL, Levy J, Perrin S, Bunn HF. Clonality in myeloproliferative disorders: analysis by means of the polymerase chain reaction. Proc Natl Acad Sci U S A. 1991;88, 6848-6852."},{"key":"2020021217543954700_REF18","doi-asserted-by":"crossref","unstructured":"Albitar M, Wu WC, Feltz E, et al. Simplified reverse dot blot analyses for detecting Ras oncogene mutations. Molecular Diagnosis. 1977;2: 169-176.","DOI":"10.1016\/S1084-8592(97)80025-2"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/103\/7\/2799\/1697018\/zh800704002799.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/103\/7\/2799\/1697018\/zh800704002799.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T18:53:05Z","timestamp":1581533585000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/103\/7\/2799\/18255\/Relative-increase-in-leukemiaspecific-DNA-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,1]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2004,4,1]]}},"URL":"https:\/\/doi.org\/10.1182\/blood-2003-06-1840","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,1]]}}}