{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:29:35Z","timestamp":1790908175777,"version":"4.1.0"},"reference-count":63,"publisher":"American Society of Hematology","issue":"10","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Gene expression profiles of bone marrow (BM) CD34-derived megakaryocytic cells (MKs) were compared in patients with essential thrombocythemia (ET) and healthy subjects using oligonucleotide microarray analysis to identify differentially expressed genes and disease-specific transcripts. We found that proapoptotic genes such as BAX, BNIP3, and BNIP3L were down-regulated in ET MKs together with genes that are components of the mitochondrial permeability transition pore complex, a system with a pivotal role in apoptosis. Conversely, antiapoptotic genes such as IGF1-R and CFLAR were up-regulated in the malignant cells, as was the SDF1 gene, which favors cell survival. On the basis of the array results, we characterized apoptosis of normal and ET MKs by time-course evaluation of annexin-V and sub-G1 peak DNA stainings of immature and mature MKs after culture in serum-free medium with an optimal thrombopoietin concentration, and annexin-V\u2013positive MKs only, with decreasing thrombopoietin concentrations. ET MKs were more resistant to apoptosis than their normal counterparts. We conclude that imbalance between proliferation and apoptosis seems to be an important step in malignant ET megakaryocytopoiesis.<\/jats:p>","DOI":"10.1182\/blood-2003-07-2597","type":"journal-article","created":{"date-parts":[[2004,7,24]],"date-time":"2004-07-24T01:38:12Z","timestamp":1090633092000},"page":"3126-3135","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":60,"title":["Gene expression profiling of normal and malignant CD34-derived megakaryocytic cells"],"prefix":"10.1182","volume":"104","author":[{"given":"Elena","family":"Tenedini","sequence":"first","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Fagioli","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Vianelli","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier Luigi","family":"Tazzari","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Ricci","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Tagliafico","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Ricci","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Gugliotta","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Martinelli","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sante","family":"Tura","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Baccarani","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ferrari","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. Sera\u0300gnoli,\u201d Universita\u0300 di Bologna, Bologna, Italy; Servizio di Medicina Trasfusionale, Ospedale S. Orsola, Bologna; Dipartimento di Scienze Biomediche, Sezione di Chimica Biologica, Universita\u0300 di Modena e Reggio Emilia, Modena; and Arcispedale \u201cS. Maria Nuova,\u201d Unita\u0300 Operativa Ematologia, Reggio Emilia, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Catani","sequence":"additional","affiliation":[{"name":"From the Istituto di Ematologia e Oncologia Medica \u201cL. e A. 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