{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:33:50Z","timestamp":1791286430607,"version":"4.1.0"},"reference-count":50,"publisher":"American Society of Hematology","issue":"10","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The frequently occurring T-cell receptor delta (TCRD) deletions in precursor-B\u2013acute lymphoblastic leukemia (precursor-B\u2013ALL) are assumed to be mainly caused by V\u03b42-J\u03b1 rearrangements. We designed a multiplex polymerase chain reaction tified clonal V\u03b42-J\u03b1 rearrangements in 141 of 339 (41%) childhood and 8 of 22 (36%) adult precursor-B\u2013ALL. A significant proportion (44%) of V\u03b42-J\u03b1 rearrangements in childhood precursor-B\u2013ALL were oligoclonal. Sequence analysis showed preferential usage of the J\u03b129 gene segment in 54% of rearrangements. The remaining V\u03b42-J\u03b1 rearrangements used 26 other J\u03b1 segments, which included 2 additional clusters, one involv ing the most upstream J\u03b1 segments (ie, J\u03b148 to J\u03b161; 23%) and the second cluster located around the J\u03b19 gene segment (7%). Real-time quantitative PCR studies of normal lymphoid cells showed that V\u03b42 rearrangements to upstream J\u03b1 segments occurred at low levels in the thymus (10\u20132 to 10\u20133) and were rare (generally below 10\u20133) in B-cell precursors and mature T cells. V\u03b42-J\u03b129 rearrangements were virtually absent in normal lymphoid cells. The monoclonal V\u03b42-J\u03b1 rearrangements in precursor-B\u2013ALL may serve as patient-specific targets for detection of minimal residual disease, because they show high sensitivity (10\u20134 or less in most cases) and good stability (88% of rearrangements preserved at relapse).<\/jats:p>","DOI":"10.1182\/blood-2003-08-2952","type":"journal-article","created":{"date-parts":[[2003,12,9]],"date-time":"2003-12-09T01:23:13Z","timestamp":1070932993000},"page":"3798-3804","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":63,"title":["V\u03b42-J\u03b1 rearrangements are frequent in precursor-B\u2013acute lymphoblastic leukemia but rare in normal lymphoid cells"],"prefix":"10.1182","volume":"103","author":[{"given":"Tomasz","family":"Szczepan\u0301ski","sequence":"first","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent H. J.","family":"van der Velden","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia G.","family":"Hoogeveen","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaike","family":"de Bie","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danie\u0308lle C. H.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth R.","family":"van Wering","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. M.","family":"van Dongen","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, Erasmus MC, University Medical Center Rotterdam, the Netherlands; Department of Pediatric Hematology and Oncology, Silesian Medical Academy, Zabrze, Poland; and Dutch Childhood Oncology Group, The Hague, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021217281770300_REF1","doi-asserted-by":"crossref","unstructured":"Davis MM, Bjo\u0308rkman PJ. T-cell antigen receptor genes and T-cell recognition. Nature. 1988;334: 395-402.","DOI":"10.1038\/334395a0"},{"key":"2020021217281770300_REF2","unstructured":"Van Dongen JJM, Comans-Bitter WM, Wolvers-Tettero ILM, Borst J. Development of human T lymphocytes and their thymus-dependency. Thymus. 1990;16: 207-234."},{"key":"2020021217281770300_REF3","doi-asserted-by":"crossref","unstructured":"Blom B, Verschuren MC, Heemskerk MH, et al. TCR gene rearrangements and expression of the pre-T cell receptor complex during human T-cell differentiation. Blood. 1999;93: 3033-3043.","DOI":"10.1182\/blood.V93.9.3033"},{"key":"2020021217281770300_REF4","doi-asserted-by":"crossref","unstructured":"Felix CA, Poplack DG, Reaman GH, et al. Characterization of immunoglobulin and T-cell receptor gene patterns in B-cell precursor acute lymphoblastic leukemia of childhood. J Clin Oncol. 1990;8: 431-442.","DOI":"10.1200\/JCO.1990.8.3.431"},{"key":"2020021217281770300_REF5","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Beishuizen A, Pongers-Willemse MJ, et al. Cross-lineage T cell receptor gene rearrangements occur in more than ninety percent of childhood precursor-B acute lymphoblastic leukemias: alternative PCR targets for detection of minimal residual disease. Leukemia. 1999;13: 196-205.","DOI":"10.1038\/sj.leu.2401277"},{"key":"2020021217281770300_REF6","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Langerak AW, Wolvers-Tettero ILM, et al. Immunoglobulin and T cell receptor gene rearrangement patterns in acute lymphoblastic leukemia are less mature in adults than in children: implications for selection of PCR targets for detection of minimal residual disease. Leukemia. 1998;12: 1081-1088.","DOI":"10.1038\/sj.leu.2401071"},{"key":"2020021217281770300_REF7","doi-asserted-by":"crossref","unstructured":"Biondi A, Francia di Celle P, Rossi V, et al. High prevalence of T-cell receptor V delta 2-(D)-D delta 3 or D delta 1\/2-D delta 3 rearrangements in B-precursor acute lymphoblastic leukemias. Blood. 1990;75: 1834-1840.","DOI":"10.1182\/blood.V75.9.1834.1834"},{"key":"2020021217281770300_REF8","doi-asserted-by":"crossref","unstructured":"Breit TM, Wolvers-Tettero ILM, Beishuizen A, Verhoeven M-AJ, van Wering ER, van Dongen JJM. Southern blot patterns, frequencies and junctional diversity of T-cell receptor \u03b4 gene rearrangements in acute lymphoblastic leukemia. Blood. 1993;82: 3063-3074.","DOI":"10.1182\/blood.V82.10.3063.3063"},{"key":"2020021217281770300_REF9","doi-asserted-by":"crossref","unstructured":"Steenbergen EJ, Verhagen OJ, van Leeuwen EF, van den Berg H, von dem Borne AE, van der Schoot CE. Frequent ongoing T-cell receptor rearrangements in childhood B-precursor acute lymphoblastic leukemia: implications for monitoring minimal residual disease. Blood. 1995;86: 692-702.","DOI":"10.1182\/blood.V86.2.692.bloodjournal862692"},{"key":"2020021217281770300_REF10","doi-asserted-by":"crossref","unstructured":"Krejci O, Prouzova Z, Horvath O, Trka J, Hrusak O. TCR delta gene is frequently rearranged in adult B lymphocytes. J Immunol. 2003;171: 524-527.","DOI":"10.4049\/jimmunol.171.2.524"},{"key":"2020021217281770300_REF11","doi-asserted-by":"crossref","unstructured":"Langerak AW, Wolvers-Tettero ILM, van Gastel-Mol EJ, Oud ME, van Dongen JJM. Basic helix-loop-helix proteins E2A and HEB induce immature T-cell receptor rearrangements in nonlymphoid cells. Blood. 2001;98: 2456-2465.","DOI":"10.1182\/blood.V98.8.2456"},{"key":"2020021217281770300_REF12","doi-asserted-by":"crossref","unstructured":"Hara J, Benedict SH, Mak TW, Gelfand EW. T cell receptor alpha-chain gene rearrangements in B-precursor leukemia are in contrast to the findings in T cell acute lymphoblastic leukemia. Comparative study of T cell receptor gene rearrangement in childhood leukemia. J Clin Invest. 1987;80: 1770-1777.","DOI":"10.1172\/JCI113270"},{"key":"2020021217281770300_REF13","doi-asserted-by":"crossref","unstructured":"Yokota S, Hansen-Hagge TE, Bartram CR. T-cell receptor delta gene recombination in common acute lymphoblastic leukemia: preferential usage of V delta 2 and frequent involvement of the J alpha cluster. Blood. 1991;77: 141-148.","DOI":"10.1182\/blood.V77.1.141.141"},{"key":"2020021217281770300_REF14","doi-asserted-by":"crossref","unstructured":"Hara J, Kawa-Ha K, Takihara Y, et al. Developmental process of the T-cell receptor alpha and delta gene assembly in B-cell precursor acute lymphoblastic leukaemia. Br J Haematol. 1991;78: 180-186.","DOI":"10.1111\/j.1365-2141.1991.tb04414.x"},{"key":"2020021217281770300_REF15","doi-asserted-by":"crossref","unstructured":"Verschuren MC, Wolvers-Tettero ILM, Breit TM, van Dongen JJM. T-cell receptor V delta-J alpha rearrangements in human thymocytes: the role of V delta-J alpha rearrangements in T-cell receptor-delta gene deletion. Immunology. 1998;93: 208-212.","DOI":"10.1046\/j.1365-2567.1998.00417.x"},{"key":"2020021217281770300_REF16","doi-asserted-by":"crossref","unstructured":"Verschuren MC, Wolvers-Tettero ILM, Breit TM, Noordzij J, van Wering ER, van Dongen JJM. Preferential rearrangements of the T cell receptor-delta-deleting elements in human T cells. J Immunol. 1997;158: 1208-1216.","DOI":"10.4049\/jimmunol.158.3.1208"},{"key":"2020021217281770300_REF17","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Orfao A, van der Velden VHJ, San Miguel JF, van Dongen JJM. Minimal residual disease in leukaemia patients. Lancet Oncol. 2001;2: 409-417.","DOI":"10.1016\/S1470-2045(00)00418-6"},{"key":"2020021217281770300_REF18","unstructured":"Van Dongen JJM, Adriaansen HJ, Hooijkaas H. Immunophenotyping of leukaemias and nonHodgkin's lymphomas. Immunological markers and their CD codes. Neth J Med. 1988;33: 298-314."},{"key":"2020021217281770300_REF19","doi-asserted-by":"crossref","unstructured":"Van Dongen JJM, Wolvers-Tettero ILM. Analysis of immunoglobulin and T cell receptor genes. Part I: basic and technical aspects. Clin Chim Acta. 1991;198: 1-91.","DOI":"10.1016\/0009-8981(91)90246-9"},{"key":"2020021217281770300_REF20","doi-asserted-by":"crossref","unstructured":"Verhagen OJHM, Wijkhuijs AJM, van der Sluys-Gelling AJ, et al. Suitable DNA isolation method for the detection of minimal residual disease by PCR techniques. Leukemia. 1999;13: 1298-1299.","DOI":"10.1038\/sj.leu.2401451"},{"key":"2020021217281770300_REF21","doi-asserted-by":"crossref","unstructured":"Van Dongen JJM, Langerak AW, Bru\u0308ggemann M, et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2003;17: 2257-2317.","DOI":"10.1038\/sj.leu.2403202"},{"key":"2020021217281770300_REF22","doi-asserted-by":"crossref","unstructured":"Koop BF, Rowen L, Wang K, et al. The human T-cell receptor TCRAC\/TCRDC (C alpha\/C delta) region: organization, sequence, and evolution of 97.6 kb of DNA. Genomics. 1994;19: 478-493.","DOI":"10.1006\/geno.1994.1097"},{"key":"2020021217281770300_REF23","doi-asserted-by":"crossref","unstructured":"Pongers-Willemse MJ, Seriu T, Stolz F, et al. Primers and protocols for standardized detection of minimal residual disease in acute lymphoblastic leukemia using immunoglobulin and T cell receptor gene rearrangements and TAL1 deletions as PCR targets: report of the BIOMED-1 Concerted Action: investigation of minimal residual disease in acute leukemia. Leukemia. 1999;13: 110-118.","DOI":"10.1038\/sj.leu.2401245"},{"key":"2020021217281770300_REF24","doi-asserted-by":"crossref","unstructured":"Langerak AW, Szczepanski T, van der Burg M, Wolvers-Tettero ILM, van Dongen JJM. Heteroduplex PCR analysis of rearranged T cell receptor genes for clonality assessment in suspect T cell proliferations. Leukemia. 1997;11: 2192-2199.","DOI":"10.1038\/sj.leu.2400887"},{"key":"2020021217281770300_REF25","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Willemse MJ, Brinkhof B, van Wering ER, van der Burg M, van Dongen JJM. Comparative analysis of Ig and TCR gene rearrangements at diagnosis and at relapse of childhood precursor-B\u2013ALL provides improved strategies for selection of stable PCR targets for monitoring of minimal residual disease. Blood. 2002;99: 2315-2323.","DOI":"10.1182\/blood.V99.7.2315"},{"key":"2020021217281770300_REF26","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Willemse MJ, Kamps WA, van Wering ER, Langerak AW, van Dongen JJM. Molecular discrimination between relapsed and secondary acute lymphoblastic leukemia: proposal for an easy strategy. Med Pediatr Oncol. 2001;36: 352-358.","DOI":"10.1002\/mpo.1085"},{"key":"2020021217281770300_REF27","doi-asserted-by":"crossref","unstructured":"Beishuizen A, de Bruijn MAC, Pongers-Willemse MJ, et al. Heterogeneity in junctional regions of immunoglobulin kappa deleting element rearrangements in B cell leukemias: a new molecular target for detection of minimal residual disease. Leukemia. 1997;11: 2200-2207.","DOI":"10.1038\/sj.leu.2400904"},{"key":"2020021217281770300_REF28","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Pongers-Willemse MJ, Langerak AW, et al. Ig heavy chain gene rearrangements in T-cell acute lymphoblastic leukemia exhibit predominant DH6-19 and DH7-27 gene usage, can result in complete V-D-J rearrangements, and are rare in T-cell receptor \u03b1\u03b2 lineage. Blood. 1999;93: 4079-4085.","DOI":"10.1182\/blood.V93.12.4079"},{"key":"2020021217281770300_REF29","unstructured":"Breit TM, Van Dongen JJM. Unravelling human T-cell receptor junctional region sequences. Thymus. 1994;22: 177-199."},{"key":"2020021217281770300_REF30","doi-asserted-by":"crossref","unstructured":"Van Wering ER, van der Linden-Schrever BE, Szczepanski T, et al. Regenerating normal B-cell precursors during and after treatment of acute lymphoblastic leukaemia: implications for monitoring of minimal residual disease. Br J Haematol. 2000;110: 139-146.","DOI":"10.1046\/j.1365-2141.2000.02143.x"},{"key":"2020021217281770300_REF31","doi-asserted-by":"crossref","unstructured":"Van Lochem EG, Wiegers YM, van den Beemd R, Ha\u0308hlen K, van Dongen JJM, Hooijkaas H. Regeneration pattern of precursor-B-cells in bone marrow of acute lymphoblastic leukemia patients depends on the type of preceding chemotherapy. Leukemia. 2000;14: 688-695.","DOI":"10.1038\/sj.leu.2401749"},{"key":"2020021217281770300_REF32","doi-asserted-by":"crossref","unstructured":"Pongers-Willemse MJ, Verhagen OJHM, Tibbe GJM, et al. Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes. Leukemia. 1998;12: 2006-2014.","DOI":"10.1038\/sj.leu.2401246"},{"key":"2020021217281770300_REF33","doi-asserted-by":"crossref","unstructured":"Moppett J, van der Velden VHJ, Wijkhuijs AJ, Hancock J, van Dongen JJM, Goulden N. Inhibition affecting RQ-PCR-based assessment of minimal residual disease in acute lymphoblastic leukemia: reversal by addition of bovine serum albumin. Leukemia. 2003;17: 268-270.","DOI":"10.1038\/sj.leu.2402751"},{"key":"2020021217281770300_REF34","doi-asserted-by":"crossref","unstructured":"Verhagen OJHM, Willemse MJ, Breunis WB, et al. Application of germline IGH probes in real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia. Leukemia. 2000;14: 1426-1435.","DOI":"10.1038\/sj.leu.2401801"},{"key":"2020021217281770300_REF35","doi-asserted-by":"crossref","unstructured":"Van der Velden VHJ, Wijkhuijs JM, Jacobs DCH, van Wering ER, van Dongen JJM. T cell receptor gamma gene rearrangements as targets for detection of minimal residual disease in acute lymphoblastic leukemia by real-time quantitative PCR analysis. Leukemia. 2002;16: 1372-1380.","DOI":"10.1038\/sj.leu.2402515"},{"key":"2020021217281770300_REF36","doi-asserted-by":"crossref","unstructured":"Van der Velden VHJ, Willemse MJ, van der Schoot CE, van Wering ER, van Dongen JJM. Immunoglobulin kappa deleting element rearrangements in precursor-B acute lymphoblastic leukemia are stable targets for detection of minimal residual disease by real-time quantitative PCR. Leukemia. 2002;16: 928-936.","DOI":"10.1038\/sj.leu.2402475"},{"key":"2020021217281770300_REF37","doi-asserted-by":"crossref","unstructured":"Van der Velden VHJ, Hochhaus A, Cazzaniga G, Szczepanski T, Gabert J, Van Dongen JJM. Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects. Leukemia. 2003;17: 1013-1034.","DOI":"10.1038\/sj.leu.2402922"},{"key":"2020021217281770300_REF38","doi-asserted-by":"crossref","unstructured":"Ghali DW, Panzer S, Fischer S, et al. Heterogeneity of the T-cell receptor delta gene indicating subclone formation in acute precursor B-cell leukemias. Blood. 1995;85: 2795-2801.","DOI":"10.1182\/blood.V85.10.2795.bloodjournal85102795"},{"key":"2020021217281770300_REF39","doi-asserted-by":"crossref","unstructured":"De Haas V, Verhagen OJ, von dem Borne AE, Kroes W, van den Berg H, van der Schoot CE. Quantification of minimal residual disease in children with oligoclonal B-precursor acute lymphoblastic leukemia indicates that the clones that grow out during relapse already have the slowest rate of reduction during induction therapy. Leukemia. 2001;15: 134-140.","DOI":"10.1038\/sj.leu.2401970"},{"key":"2020021217281770300_REF40","doi-asserted-by":"crossref","unstructured":"Konrad M, Metzler M, Panzer S, et al. Late relapses evolve from slow-responding subclones in t(12;21)-positive acute lymphoblastic leukemia: evidence for the persistence of a preleukemic clone. Blood. 2003;101: 3635-3640.","DOI":"10.1182\/blood-2002-10-3252"},{"key":"2020021217281770300_REF41","doi-asserted-by":"crossref","unstructured":"Li A, Zhou J, Zuckerman D, et al. Sequence analysis of clonal immunoglobulin and T-cell receptor gene rearrangements in children with acute lymphoblastic leukemia at diagnosis and at relapse: implications for pathogenesis and for the clinical utility of PCR-based methods of minimal residual disease detection. Blood. 2003;102: 4520-4526.","DOI":"10.1182\/blood-2003-05-1455"},{"key":"2020021217281770300_REF42","doi-asserted-by":"crossref","unstructured":"Bierings M, Szczepanski T, van Wering ER, et al. Two consecutive immunophenotypic switches in a child with immunogenotypically stable acute leukaemia. Br J Haematol. 2001;113: 757-762.","DOI":"10.1046\/j.1365-2141.2001.02772.x"},{"key":"2020021217281770300_REF43","doi-asserted-by":"crossref","unstructured":"Asnafi V, Radford-Weiss I, Dastugue N, et al. CALM-AF10 is a common fusion transcript in T-ALL and is specific to the TCR\u03b3\u03b4 lineage. Blood. 2003;102: 1000-1006.","DOI":"10.1182\/blood-2002-09-2913"},{"key":"2020021217281770300_REF44","doi-asserted-by":"crossref","unstructured":"Seeger K, Taube T, Eckert C, Hanel C, Pogodda M, Henze G. Unusual T-cell receptor-delta gene rearrangement patterns revealed by screening of a large series of childhood acute lymphoblastic leukaemia by multiplex polymerase chain reaction. Br J Haematol. 2001;113: 318-322.","DOI":"10.1046\/j.1365-2141.2001.02732.x"},{"key":"2020021217281770300_REF45","doi-asserted-by":"crossref","unstructured":"Breit TM, Verschuren MCM, Wolvers-Tettero ILM, van Gastel-Mol EJ, Ha\u0308hlen K, van Dongen JJM. Human T cell leukemias with continuous V(D)J recombinase activity for TCR-delta gene deletion. J Immunol. 1997;159: 4341-4349.","DOI":"10.4049\/jimmunol.159.9.4341"},{"key":"2020021217281770300_REF46","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Flohr T, van der Velden VHJ, Bartram CR, van Dongen JJM. Molecular monitoring of residual disease using antigen receptor genes in childhood acute lymphoblastic leukaemia. Best Pract Res Clin Haematol. 2002;15: 37-57.","DOI":"10.1053\/beha.2002.0184"},{"key":"2020021217281770300_REF47","doi-asserted-by":"crossref","unstructured":"Van Dongen JJM, Seriu T, Panzer-Gru\u0308mayer ER, et al. Prognostic value of minimal residual disease in acute lymphoblastic leukaemia in childhood. Lancet. 1998;352: 1731-1738.","DOI":"10.1016\/S0140-6736(98)04058-6"},{"key":"2020021217281770300_REF48","doi-asserted-by":"crossref","unstructured":"Willemse MJ, Seriu T, Hettinger K, et al. Detection of minimal residual disease identifies differences in treatment response between T-ALL and precursor B-ALL. Blood. 2002;99: 4386-4393.","DOI":"10.1182\/blood.V99.12.4386"},{"key":"2020021217281770300_REF49","doi-asserted-by":"crossref","unstructured":"Szczepanski T, Willemse MJ, van Wering ER, Weerden JF, Kamps WA, van Dongen JJM. Precursor-B\u2013ALL with DH-JH gene rearrangements have an immature immunogenotype with a high frequency of oligoclonality and hyperdiploidy of chromosome 14. Leukemia. 2001;15: 1415-1423.","DOI":"10.1038\/sj.leu.2402206"},{"key":"2020021217281770300_REF50","doi-asserted-by":"crossref","unstructured":"Van der Velden VHJ, Szczepanski T, Wijkhuijs JM, et al. Age-related patterns of immunoglobulin and T-cell receptor gene rearrangements in precursor-B\u2013ALL: implications for detection of minimal residual disease. Leukemia. 2003;17: 1834-1844.","DOI":"10.1038\/sj.leu.2403038"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/103\/10\/3798\/1697021\/zh801004003798.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/103\/10\/3798\/1697021\/zh801004003798.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T16:11:52Z","timestamp":1682611912000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/103\/10\/3798\/17618\/V%CE%B42J%CE%B1-rearrangements-are-frequent-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,15]]},"references-count":50,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,5,15]]}},"URL":"https:\/\/doi.org\/10.1182\/blood-2003-08-2952","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5,15]]}}}