{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:39:48Z","timestamp":1791398388539,"version":"4.3.3"},"reference-count":26,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanisms responsible for immunoglobulin G (IgG) immunity against allogeneic platelets are poorly understood. We studied the role that murine recipient CD8+ T and natural killer (NK) cells play in immunity against allogeneic platelets. BALB\/c mice were depleted of the cells by cell-specific antibodies, transfused weekly with platelets from C57BL\/6 mice, and serum IgG antidonor antibodies were measured by flow cytometry. While allogeneic platelet transfusions into wild-type recipients stimulated IgG antidonor antibodies in all mice by the fifth transfusion, CD8-depleted mice had significantly (P &amp;lt; .001) enhanced antibody production. Isotype analysis revealed that CD8+ T cells suppressed T-helper 2 (Th2)-associated IgG1 but enhanced Th1-associated IgG2a. Compared with wild-type mice, platelet transfusions into CD8-depleted mice stimulated enhanced intracellular interferon (IFN)-\u03b3 production by CD4- lymphocytes within 24 hours after the first transfusion. The early IFN-\u03b3 response correlated with nitric oxide-dependent splenic cytotoxicity (P &amp;lt; .001). In asialo ganglioside monosialic acid 1 (GM1)-depleted mice transfused with allogeneic platelets, the IFN-\u03b3 production, splenic cytotoxicity, and IgG antidonor antibody response were significantly suppressed. These results demonstrate that IgG antiplatelet immunity is dependent on an early NK cell-derived IFN-\u03b3 response that is negatively regulated by CD8+ T cells and suggest that targeting innate NK cell responses may significantly reduce platelet alloimmunization. (Blood. 2004;103:2705-2709)<\/jats:p>","DOI":"10.1182\/blood-2003-10-3552","type":"journal-article","created":{"date-parts":[[2003,12,9]],"date-time":"2003-12-09T01:23:13Z","timestamp":1070932993000},"page":"2705-2709","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":42,"title":["IgG antiplatelet immunity is dependent on an early innate natural killer cell\u2013derived interferon-\u03b3 response that is regulated by CD8+ T cells"],"prefix":"10.1182","volume":"103","author":[{"given":"Ebrahim","family":"Sayeh","sequence":"first","affiliation":[{"name":"From the Department of Laboratory Medicine and Pathobiology, St Michael's Hospital, Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services and the Toronto Platelet Immunobiology Group, Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Sterling","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine and Pathobiology, St Michael's Hospital, Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services and the Toronto Platelet Immunobiology Group, Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Speck","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine and Pathobiology, St Michael's Hospital, Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services and the Toronto Platelet Immunobiology Group, Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Freedman","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine and Pathobiology, St Michael's Hospital, Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services and the Toronto Platelet Immunobiology Group, Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Semple","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine and Pathobiology, St Michael's Hospital, Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services and the Toronto Platelet Immunobiology Group, Toronto, ON, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021218103471500_REF1","doi-asserted-by":"crossref","unstructured":"Karger R, Kretschmer V. 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