{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T23:47:57Z","timestamp":1696463277367},"reference-count":32,"publisher":"American Society of Hematology","issue":"15","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,10,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>After administration of granulocyte colony-stimulating factor (G-CSF), there is a marked, albeit transient, drop in circulating neutrophils. To determine the role of leukocyte integrins in this disappearance, a dog having canine leukocyte adhesion deficiency (CLAD) or CLAD dogs who had undergone gene correction either by matched littermate allogeneic transplant or autologous gene therapy were evaluated. Shortly after G-CSF administration, a dramatic, yet transient, neutropenia was observed in the control littermates. This neutropenia was not as marked in the CLAD dogs. In all instances, it was CD18+ neutrophils that preferentially egressed from the circulation. The association of CD18 with this rapid loss suggested leukocyte integrin activation after G-CSF administration. To determine the activation status of the integrin, a monoclonal antibody recognizing the activated \u03b1-subunit cation binding domain (mAb24) was used to evaluate human leukocytes after G-CSF administration. Mirroring the dramatic decrease in circulating neutrophil numbers, there was a dramatic and specific increase in the activation of the \u03b1-subunit after G-CSF expression on polymorphonuclear leukocytes. This activation, like the drop in neutrophil count, was transient. These results demonstrate that the leukocyte integrin on circulating neutrophils is transiently activated after G-CSF administration and mediates the transient neutropenia observed after G-CSF administration.<\/jats:p>","DOI":"10.1182\/blood-2011-06-360461","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T01:02:36Z","timestamp":1313456556000},"page":"4209-4214","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":5,"title":["Leukocyte integrin activation mediates transient neutropenia after G-CSF administration"],"prefix":"10.1182","volume":"118","author":[{"given":"Robert E.","family":"Donahue","sequence":"first","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, Rockville, MD;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Tuschong","sequence":"additional","affiliation":[{"name":"Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Thomas R.","family":"Bauer","sequence":"additional","affiliation":[{"name":"Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu Ying","family":"Yau","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan F.","family":"Leitman","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis 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