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No alteration in neutrophil apoptosis was observed, implicating impaired recruitment. Indeed, Ly6G ligation abrogated neutrophil migration toward LTB4 and other chemoattractants in a transwell system. Exploring the basis for this blockade, we identified colocalization of Ly6G and \u03b22-integrins by confocal microscopy and confirmed close association by both coimmunoprecipitation and fluorescence lifetime imaging microscopy. Anti-Ly6G Ab impaired surface expression of \u03b22-integrins in LTB4-stimulated neutrophils and mimicked CD11a blockade in inhibiting both ICAM-1 binding and firm adhesion to activated endothelium under flow conditions. Correspondingly, migration of \u03b22-integrin\u2013deficient neutrophils was no longer inhibited by anti-Ly6G. These results demonstrate that experimental targeting of Ly6G has functional effects on the neutrophil population and identify a previously unappreciated role for Ly6G as a modulator of neutrophil migration to sites of inflammation via a \u03b22-integrin\u2013dependent mechanism.<\/jats:p>","DOI":"10.1182\/blood-2012-01-404046","type":"journal-article","created":{"date-parts":[[2012,6,2]],"date-time":"2012-06-02T00:26:27Z","timestamp":1338596787000},"page":"1489-1498","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":116,"title":["Ly6G ligation blocks recruitment of neutrophils via a \u03b22-integrin\u2013dependent mechanism"],"prefix":"10.1182","volume":"120","author":[{"given":"Jun-Xia","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Bair","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Nephrology, Massachusetts General Hospital, Harvard Medical School, Boston, MA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra L.","family":"King","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruslan","family":"Shnayder","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine and Department of Pediatrics, National Cheng-Kung University, Tainan, Taiwan; 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