{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T23:57:01Z","timestamp":1776988621741,"version":"3.51.4"},"reference-count":19,"publisher":"American Society of Hematology","issue":"15","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,10,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A 2-day method using flow cytometry and FISH for interphase cells was developed to detect monosomy 7 cells in myelodysplastic syndrome patients. The method, Interphase Chromosome Flow-FISH (IC Flow-FISH), involves fixation of leukocytes from blood, membrane permeabilization, hybridization of cellular DNA with peptide nucleic acid probes with cells intact, and analysis by flow cytometry. Hundreds to thousands of monosomy 7 cells were consistently detected from 10-20 mL of blood in patients with monosomy 7. Proportions of monosomy 7 cells detected in IC Flow-FISH were compared with results from conventional cytogenetics; identification of monosomy 7 populations was verified with FACS; and patient and donor cells were mixed to test for sensitivity. IC Flow-FISH allows for detecting monosomy 7 without requiring bone marrow procurement or the necessity of metaphase spreads, and wider applications to other chromosomal abnormalities are in development.<\/jats:p>","DOI":"10.1182\/blood-2012-05-434266","type":"journal-article","created":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T00:19:59Z","timestamp":1346285999000},"page":"e54-e59","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":6,"title":["Interphase Chromosome Flow-FISH"],"prefix":"10.1182","volume":"120","author":[{"given":"Keyvan","family":"Keyvanfar","sequence":"first","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Weed","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashanth","family":"Swamy","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Kajigaya","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo T.","family":"Calado","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"},{"name":"Department of Internal Medicine, University of S\u00e3o Paulo at Ribeir\u00e3o Preto Medical School, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neal S.","family":"Young","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"issue":"12","key":"2019111808294817800_B1","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/S0145-2126(03)00104-8","article-title":"Comparison of interphase FISH and metaphase cytogenetics to study myelodysplastic syndrome: an Eastern Cooperative Oncology Group (ECOG) study.","volume":"27","author":"Cherry","year":"2003","journal-title":"Leuk Res"},{"issue":"1","key":"2019111808294817800_B2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.cancergencyto.2004.08.012","article-title":"The value of fluorescence in situ hybridization in the diagnosis and prognosis of chronic lymphocytic leukemia.","volume":"158","author":"Glassman","year":"2005","journal-title":"Cancer Genet Cytogenet"},{"issue":"7","key":"2019111808294817800_B3","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1182\/blood.V92.7.2322","article-title":"The importance of diagnostic cytogenetics on outcome in AML: analysis of 1612 patients entered into the MRC AML 10 trial. 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