{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:14:43Z","timestamp":1788488083687,"version":"build-2803163510"},"reference-count":64,"publisher":"American Society of Hematology","issue":"23","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,6,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sickle cell disease (SCD) is associated with chronic activation of coagulation and an increased risk of venous thromboembolism. Erythrocyte sickling, the primary pathologic event in SCD, results in dramatic morphological changes in red blood cells (RBCs) because of polymerization of the abnormal hemoglobin. We used a mouse model of SCD and blood samples from sickle patients to determine if these changes affect the structure, properties, and dynamics of sickle clot formation. Sickling of RBCs and a significant increase in fibrin deposition were observed in venous thrombi formed in sickle mice. During ex vivo clot contraction, the number of RBCs extruded from sickle whole blood clots was significantly reduced compared with the number released from sickle cell trait and nonsickle clots in both mice and humans. Entrapment of sickled RBCs was largely factor XIIIa\u2013independent and entirely mediated by the platelet-free cellular fraction of sickle blood. Inhibition of phosphatidylserine, but not administration of antisickling compounds, increased the number of RBCs released from sickle clots. Interestingly, whole blood, but not plasma clots from SCD patients, was more resistant to fibrinolysis, indicating that the cellular fraction of blood mediates resistance to tissue plasminogen activator. Sickle trait whole blood clots demonstrated an intermediate phenotype in response to tissue plasminogen activator. RBC exchange in SCD patients had a long-lasting effect on normalizing whole blood clot contraction. Furthermore, RBC exchange transiently reversed resistance of whole blood sickle clots to fibrinolysis, in part by decreasing platelet-derived PAI-1. These properties of sickle clots may explain the increased risk of venous thromboembolism observed in SCD.<\/jats:p>","DOI":"10.1182\/blood.2019000424","type":"journal-article","created":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T21:10:12Z","timestamp":1554498612000},"page":"2529-2541","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":76,"title":["Red blood cells modulate structure and dynamics of venous clot formation in sickle cell disease"],"prefix":"10.1182","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7047-2482","authenticated-orcid":false,"given":"Camille","family":"Faes","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"},{"name":"Interuniversity Laboratory of Human Movement Biology EA7424, University Lyon\u2013University Claude Bernard Lyon 1, Villeurbanne, France;"},{"name":"Labex GR-Ex, Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7669-0760","authenticated-orcid":false,"given":"Anton","family":"Ilich","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Sotiaux","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica M.","family":"Sparkenbaugh","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Buczek","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7903-944X","authenticated-orcid":false,"given":"Joan D.","family":"Beckman","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Ellsworth","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis F.","family":"Noubouossie","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lantarima","family":"Bhoopat","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Piegore","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Renoux","sequence":"additional","affiliation":[{"name":"Interuniversity Laboratory of Human Movement Biology EA7424, University Lyon\u2013University Claude Bernard Lyon 1, Villeurbanne, France;"},{"name":"Labex GR-Ex, Paris, France;"},{"name":"Biochemistry and Molecular Biology Laboratory, Functional Unit of Biochemistry and Erythrocyte Pathologies, East Center for Biology and Pathology, Health Center of Lyon, Lyon, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Bergmeier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth I.","family":"Ataga","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Cooley","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2845-2303","authenticated-orcid":false,"given":"Alisa S.","family":"Wolberg","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel S.","family":"Key","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"},{"name":"Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafal","family":"Pawlinski","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"issue":"1","key":"2020021109210475900_B1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/bjh.12336","article-title":"Interplay between coagulation and vascular inflammation in sickle cell disease","volume":"162","author":"Sparkenbaugh","year":"2013","journal-title":"Br J Haematol"},{"issue":"7","key":"2020021109210475900_B2","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1111\/jth.13717","article-title":"Prothrombotic aspects of sickle cell disease","volume":"15","author":"Sparkenbaugh","year":"2017","journal-title":"J Thromb Haemost"},{"issue":"3","key":"2020021109210475900_B3","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1182\/blood-2012-04-424143","article-title":"Tissue factor promotes activation of coagulation and inflammation in a mouse model of sickle cell disease","volume":"120","author":"Chantrathammachart","year":"2012","journal-title":"Blood"},{"issue":"11","key":"2020021109210475900_B4","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1182\/blood-2013-08-523936","article-title":"Differential contribution of FXa and thrombin to vascular inflammation in a mouse model of sickle cell disease","volume":"123","author":"Sparkenbaugh","year":"2014","journal-title":"Blood"},{"issue":"10","key":"2020021109210475900_B5","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1182\/blood-2015-11-681114","article-title":"Thrombin-independent contribution of tissue factor to inflammation and cardiac hypertrophy in a mouse model of sickle cell disease","volume":"127","author":"Sparkenbaugh","year":"2016","journal-title":"Blood"},{"issue":"15","key":"2020021109210475900_B6","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1182\/blood-2015-01-625707","article-title":"Genetic diminution of circulating prothrombin ameliorates multiorgan pathologies in sickle cell disease mice","volume":"126","author":"Arumugam","year":"2015","journal-title":"Blood"},{"issue":"4","key":"2020021109210475900_B7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.blre.2015.12.003","article-title":"Coagulation abnormalities of sickle cell disease: Relationship with clinical outcomes and the effect of disease modifying therapies","volume":"30","author":"Noubouossie","year":"2016","journal-title":"Blood Rev"},{"issue":"12","key":"2020021109210475900_B8","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1111\/jth.12744","article-title":"Venous thromboembolism incidence in the Cooperative Study of Sickle Cell Disease","volume":"12","author":"Naik","year":"2014","journal-title":"J Thromb Haemost"},{"key":"2020021109210475900_B9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.thromres.2016.03.003","article-title":"Risk factors for venous thromboembolism in adults with hemoglobin SC or S\u03b2(+) thalassemia genotypes","volume":"141","author":"Yu","year":"2016","journal-title":"Thromb Res"},{"key":"2020021109210475900_B10","doi-asserted-by":"crossref","DOI":"10.1016\/j.ajog.2008.04.016","article-title":"Morbidity associated with sickle cell disease in pregnancy","author":"Villers","year":"2008"},{"issue":"2","key":"2020021109210475900_B11","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/bjh.14655","article-title":"Increased incidence of VTE in sickle cell disease patients: risk factors, recurrence and impact on mortality","volume":"178","author":"Brunson","year":"2017","journal-title":"Br J Haematol"},{"issue":"6","key":"2020021109210475900_B12","first-page":"858","article-title":"Pulmonary changes in sickle cell disease","volume":"103","author":"Oppenheimer","year":"1971","journal-title":"Am Rev Respir Dis"},{"issue":"6","key":"2020021109210475900_B13","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1055\/s-2007-1006393","article-title":"Sickle-cell disease and the pulmonary circulation","volume":"15","author":"Kirkpatrick","year":"1994","journal-title":"Semin Respir Crit Care Med"},{"issue":"11","key":"2020021109210475900_B14","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.5858\/2001-125-1436-PTAIPW","article-title":"Pulmonary thrombotic arteriopathy in patients with sickle cell disease","volume":"125","author":"Adedeji","year":"2001","journal-title":"Arch Pathol Lab Med"},{"issue":"6","key":"2020021109210475900_B15","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1002\/ana.410200606","article-title":"Sickle cell anemia and central nervous system infarction: a neuropathological study","volume":"20","author":"Rothman","year":"1986","journal-title":"Ann Neurol"},{"issue":"5","key":"2020021109210475900_B16","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1111\/j.1540-8175.2010.01201.x","article-title":"Live\/real time three-dimensional transthoracic echocardiographic assessment of inferior vena cava and hepatic vein thrombosis in sickle cell disease","volume":"27","author":"Singh","year":"2010","journal-title":"Echocardiography"},{"issue":"5","key":"2020021109210475900_B17","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s00464-002-4529-2","article-title":"Portal vein thrombosis following laparoscopic surgery in a patient with sickle cell disease","volume":"17","author":"Ng","year":"2003","journal-title":"Surg Endosc"},{"issue":"16","key":"2020021109210475900_B18","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1182\/blood-2017-03-745349","article-title":"Red blood cells in thrombosis","volume":"130","author":"Byrnes","year":"2017","journal-title":"Blood"},{"issue":"8","key":"2020021109210475900_B19","doi-asserted-by":"crossref","first-page":"3590","DOI":"10.1172\/JCI75386","article-title":"Factor XIII activity mediates red blood cell retention in venous thrombi","volume":"124","author":"Aleman","year":"2014","journal-title":"J Clin Invest"},{"issue":"16","key":"2020021109210475900_B20","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1182\/blood-2015-06-652263","article-title":"Factor XIIIa-dependent retention of red blood cells in clots is mediated by fibrin \u03b1-chain crosslinking","volume":"126","author":"Byrnes","year":"2015","journal-title":"Blood"},{"issue":"10","key":"2020021109210475900_B21","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1182\/blood-2013-08-523860","article-title":"Clot contraction: compression of erythrocytes into tightly packed polyhedra and redistribution of platelets and fibrin","volume":"123","author":"Cines","year":"2014","journal-title":"Blood"},{"issue":"1","key":"2020021109210475900_B22","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1182\/blood-2015-05-647560","article-title":"Kinetics and mechanics of clot contraction are governed by the molecular and cellular composition of the blood","volume":"127","author":"Tutwiler","year":"2016","journal-title":"Blood"},{"issue":"2","key":"2020021109210475900_B23","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/jth.14360","article-title":"Red blood cells: the forgotten player in hemostasis and thrombosis","volume":"17","author":"Weisel","year":"2019","journal-title":"J Thromb Haemost"},{"issue":"7181","key":"2020021109210475900_B24","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1038\/nature06797","article-title":"Triggers, targets and treatments for thrombosis","volume":"451","author":"Mackman","year":"2008","journal-title":"Nature"},{"issue":"11","key":"2020021109210475900_B25","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1182\/blood-2014-01-550319","article-title":"A systems approach to hemostasis: 3. Thrombus consolidation regulates intrathrombus solute transport and local thrombin activity","volume":"124","author":"Stalker","year":"2014","journal-title":"Blood"},{"issue":"12","key":"2020021109210475900_B26","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1161\/ATVBAHA.112.300312","article-title":"Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction","volume":"32","author":"Muthard","year":"2012","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"1","key":"2020021109210475900_B27","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1182\/bloodadvances.2017011890","article-title":"Factor XIII in plasma, but not in platelets, mediates red blood cell retention in clots and venous thrombus size in mice","volume":"2","author":"Kattula","year":"2018","journal-title":"Blood Adv"},{"issue":"9757","key":"2020021109210475900_B28","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1016\/S0140-6736(10)61029-X","article-title":"Sickle-cell disease","volume":"376","author":"Rees","year":"2010","journal-title":"Lancet"},{"issue":"2","key":"2020021109210475900_B29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.blre.2015.08.005","article-title":"The role of blood rheology in sickle cell disease","volume":"30","author":"Connes","year":"2016","journal-title":"Blood Rev"},{"issue":"1","key":"2020021109210475900_B30","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0049-3848(96)00048-5","article-title":"Erythrocyte aggregation and erythrocyte deformability modify the permeability of erythrocyte enriched fibrin network","volume":"82","author":"van Gelder","year":"1996","journal-title":"Thromb Res"},{"issue":"1","key":"2020021109210475900_B31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11239-016-1426-4","article-title":"Computational imaging analysis of fibrin matrices with the inclusion of erythrocytes from homozygous SS blood reveals agglomerated and amorphous structures","volume":"43","author":"Averett","year":"2017","journal-title":"J Thromb Thrombolysis"},{"issue":"23","key":"2020021109210475900_B32","doi-asserted-by":"crossref","DOI":"10.1182\/blood.V126.23.215.215","article-title":"Clot contraction-mediated erythrocyte packing is significantly altered in sickle cell disease [abstract]","volume":"126","author":"Strauss","year":"2015","journal-title":"Blood"},{"issue":"4","key":"2020021109210475900_B33","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1182\/blood-2006-02-004812","article-title":"Correction of sickle cell disease by homologous recombination in embryonic stem cells","volume":"108","author":"Wu","year":"2006","journal-title":"Blood"},{"issue":"1","key":"2020021109210475900_B34","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/jth.13181","article-title":"Tissue factor-positive tumor microvesicles activate platelets and enhance thrombosis in mice","volume":"14","author":"Geddings","year":"2016","journal-title":"J Thromb Haemost"},{"issue":"6","key":"2020021109210475900_B35","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1161\/ATVBAHA.111.225334","article-title":"In vivo fluorescence imaging of large-vessel thrombosis in mice","volume":"31","author":"Cooley","year":"2011","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"8","key":"2020021109210475900_B36","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1161\/ATVBAHA.113.301607","article-title":"Elevated prothrombin promotes venous, but not arterial, thrombosis in mice","volume":"33","author":"Aleman","year":"2013","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"6","key":"2020021109210475900_B37","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/BF01114811","article-title":"Fixation with even small quantities of glutaraldehyde affects red blood cell surface properties in a cell- and species-dependent manner. Studies by cell partitioning","volume":"9","author":"Walter","year":"1989","journal-title":"Biosci Rep"},{"issue":"2","key":"2020021109210475900_B38","doi-asserted-by":"crossref","first-page":"021012","DOI":"10.1115\/1.4032187","article-title":"Increased red blood cell stiffness increases pulmonary vascular resistance and pulmonary arterial pressure","volume":"138","author":"Schreier","year":"2016","journal-title":"J Biomech Eng"},{"issue":"1","key":"2020021109210475900_B39","first-page":"23","volume":"57","author":"Kozanoglu","year":"2018","journal-title":"Use of red blood cell exchange for treating acute complications of sickle cell disease"},{"issue":"9","key":"2020021109210475900_B40","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1111\/trf.14154","article-title":"From total blood exchange to erythrocytapheresis and back to treat complications of sickle cell disease","volume":"57","author":"Ballas","year":"2017","journal-title":"Transfusion"},{"issue":"6","key":"2020021109210475900_B41","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1160\/TH11-10-0689","article-title":"The acceleration of the propagation phase of thrombin generation in patients with steady-state sickle cell disease is associated with circulating erythrocyte-derived microparticles","volume":"107","author":"Gerotziafas","year":"2012","journal-title":"Thromb Haemost"},{"issue":"3","key":"2020021109210475900_B42","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1159\/000362148","article-title":"Hydroxyurea increases plasma concentrations of microparticles and reduces coagulation activation and fibrinolysis in patients with sickle cell anemia","volume":"133","author":"Brunetta","year":"2015","journal-title":"Acta Haematol"},{"issue":"20","key":"2020021109210475900_B43","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1056\/NEJM199505183322001","article-title":"Effect of hydroxyurea on the frequency of painful crises in sickle cell anemia","volume":"332","author":"Charache","year":"1995","journal-title":"N Engl J Med"},{"issue":"9","key":"2020021109210475900_B44","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1111\/j.1538-7836.2012.04861.x","article-title":"Hydroxyurea is associated with reductions in hypercoagulability markers in sickle cell anemia","volume":"10","author":"Colella","year":"2012","journal-title":"J Thromb Haemost"},{"issue":"4","key":"2020021109210475900_B45","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/j.1365-2141.2004.05332.x","article-title":"5-hydroxymethyl-2-furfural modifies intracellular sickle haemoglobin and inhibits sickling of red blood cells","volume":"128","author":"Abdulmalik","year":"2005","journal-title":"Br J Haematol"},{"issue":"5","key":"2020021109210475900_B46","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1161\/ATVBAHA.118.310771","article-title":"Impaired collateral vessel formation in sickle cell disease","volume":"38","author":"Okwan-Duodu","year":"2018","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2020021109210475900_B47","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/978-3-319-49674-0_13","article-title":"Fibrin formation, structure and properties","volume":"82","author":"Weisel","year":"2017","journal-title":"Subcell Biochem"},{"issue":"23","key":"2020021109210475900_B48","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1182\/blood-2009-06-228940","article-title":"Contributions of extravascular and intravascular cells to fibrin network formation, structure, and stability","volume":"114","author":"Campbell","year":"2009","journal-title":"Blood"},{"issue":"3","key":"2020021109210475900_B49","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/j.1365-2141.2012.09065.x","article-title":"Tissue factor-positive monocytes in children with sickle cell disease: correlation with biomarkers of haemolysis","volume":"157","author":"Setty","year":"2012","journal-title":"Br J Haematol"},{"issue":"18","key":"2020021109210475900_B50","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1182\/blood-2013-09-529420","article-title":"Platelet bioenergetic screen in sickle cell patients reveals mitochondrial complex V inhibition, which contributes to platelet activation","volume":"123","author":"Cardenes","year":"2014","journal-title":"Blood"},{"issue":"12","key":"2020021109210475900_B51","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1111\/jth.12423","article-title":"The contribution of red blood cells to thrombin generation in sickle cell disease: meizothrombin generation on sickled red blood cells","volume":"11","author":"Whelihan","year":"2013","journal-title":"J Thromb Haemost"},{"issue":"3","key":"2020021109210475900_B52","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1055\/s-0037-1614486","article-title":"Contribution of erythrocytes to thrombin generation in whole blood","volume":"81","author":"Peyrou","year":"1999","journal-title":"Thromb Haemost"},{"issue":"4","key":"2020021109210475900_B53","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/j.1365-2141.2006.06047.x","article-title":"The effect of red blood cells on thrombin generation","volume":"133","author":"Horne","year":"2006","journal-title":"Br J Haematol"},{"issue":"9","key":"2020021109210475900_B54","doi-asserted-by":"crossref","first-page":"5487","DOI":"10.1073\/pnas.77.9.5487","article-title":"Determination of deoxyhemoglobin S polymer in sickle erythrocytes upon deoxygenation","volume":"77","author":"Noguchi","year":"1980","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"2020021109210475900_B55","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1001\/archinte.1974.00320160039005","article-title":"Ultrastructural features of erythrocyte and hemoglobin sickling","volume":"133","author":"White","year":"1974","journal-title":"Arch Intern Med"},{"issue":"10","key":"2020021109210475900_B56","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1111\/jth.13416","article-title":"Thrombin generation and cell-dependent hypercoagulability in sickle cell disease","volume":"14","author":"Whelihan","year":"2016","journal-title":"J Thromb Haemost"},{"issue":"3","key":"2020021109210475900_B57","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.blre.2006.11.001","article-title":"Thrombin generation and fibrin clot structure","volume":"21","author":"Wolberg","year":"2007","journal-title":"Blood Rev"},{"issue":"3","key":"2020021109210475900_B58","doi-asserted-by":"crossref","first-page":"e316","DOI":"10.1111\/j.1365-2516.2011.02648.x","article-title":"Thrombin activatable fibrinolysis inhibitor activation and bleeding in haemophilia A","volume":"18","author":"Foley","year":"2012","journal-title":"Haemophilia"},{"issue":"3","key":"2020021109210475900_B59","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1182\/blood-2006-11-057604","article-title":"Sickle cell trait and the risk of venous thromboembolism among blacks","volume":"110","author":"Austin","year":"2007","journal-title":"Blood"},{"issue":"1","key":"2020021109210475900_B60","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/jth.12787","article-title":"Prospective study of sickle cell trait and venous thromboembolism incidence","volume":"13","author":"Folsom","year":"2015","journal-title":"J Thromb Haemost"},{"issue":"9","key":"2020021109210475900_B61","doi-asserted-by":"crossref","first-page":"619","DOI":"10.7326\/M18-1161","article-title":"Clinical outcomes associated with sickle cell trait: a systematic review","volume":"169","author":"Naik","year":"2018","journal-title":"Ann Intern Med"},{"issue":"4","key":"2020021109210475900_B62","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/bjh.13641","article-title":"Coagulation activation in sickle cell trait: an exploratory study","volume":"171","author":"Amin","year":"2015","journal-title":"Br J Haematol"},{"issue":"18","key":"2020021109210475900_B63","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1182\/blood-2016-01-694422","article-title":"Elevated D-dimer levels in African Americans with sickle cell trait","volume":"127","author":"Naik","year":"2016","journal-title":"Blood"},{"issue":"9","key":"2020021109210475900_B64","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.1016\/S0021-9258(19)69413-5","article-title":"Polymerization of hemoglobin in sickle trait erythrocytes and lysates","volume":"256","author":"Noguchi","year":"1981","journal-title":"J Biol Chem"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/133\/23\/2529\/1553650\/bloodbld2019000424.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/133\/23\/2529\/1553650\/bloodbld2019000424.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T10:52:15Z","timestamp":1663239135000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/133\/23\/2529\/273844\/Red-blood-cells-modulate-structure-and-dynamics-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,6]]},"references-count":64,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2019,6,6]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2019000424","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,6]]}}}