{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T06:38:33Z","timestamp":1793342313002,"version":"4.5.0"},"reference-count":51,"publisher":"American Society of Hematology","issue":"2","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Approximately one-fourth of patients with essential thrombocythemia or primary myelofibrosis carry a somatic mutation of the calreticulin gene (CALR), the gene encoding for calreticulin. A 52-bp deletion (type I mutation) and a 5-bp insertion (type II mutation) are the most frequent genetic lesions. The mechanism(s) by which a CALR mutation leads to a myeloproliferative phenotype has been clarified only in part. We studied the interaction between calreticulin and store-operated calcium (Ca2+) entry (SOCE) machinery in megakaryocytes (Mks) from healthy individuals and from patients with CALR-mutated myeloproliferative neoplasms (MPNs). In Mks from healthy subjects, binding of recombinant human thrombopoietin to c-Mpl induced the activation of signal transducer and activator of transcription 5, AKT, and extracellular signal-regulated kinase 1\/2, determining inositol triphosphate\u2013dependent Ca2+ release from the endoplasmic reticulum (ER). This resulted in the dissociation of the ER protein 57 (ERp57)-mediated complex between calreticulin and stromal interaction molecule 1 (STIM1), a protein of the SOCE machinery that leads to Ca2+ mobilization. In Mks from patients with CALR-mutated MPNs, defective interactions between mutant calreticulin, ERp57, and STIM1 activated SOCE and generated spontaneous cytosolic Ca2+ flows. In turn, this resulted in abnormal Mk proliferation that was reverted using a specific SOCE inhibitor. In summary, the abnormal SOCE regulation of Ca2+ flows in Mks contributes to the pathophysiology of CALR-mutated MPNs. In perspective, SOCE may represent a new therapeutic target to counteract Mk proliferation and its clinical consequences in MPNs.<\/jats:p>","DOI":"10.1182\/blood.2019001103","type":"journal-article","created":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T14:43:09Z","timestamp":1571928189000},"page":"133-144","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":76,"title":["Defective interaction of mutant calreticulin and SOCE in megakaryocytes from patients with myeloproliferative neoplasms"],"prefix":"10.1182","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6472-2008","authenticated-orcid":false,"given":"Christian A.","family":"Di Buduo","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"},{"name":"Laboratory of Biochemistry, Biotechnology and Advanced Diagnosis, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo Foundation, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8066-9895","authenticated-orcid":false,"given":"Vittorio","family":"Abbonante","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"},{"name":"Laboratory of Biochemistry, Biotechnology and Advanced Diagnosis, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo Foundation, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-2302","authenticated-orcid":false,"given":"Caroline","family":"Marty","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche (UMR) 1170, Gustave Roussy, Universit\u00e9 Paris-Sud, INSERM, Villejuif, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0010-0098","authenticated-orcid":false,"given":"Francesco","family":"Moccia","sequence":"additional","affiliation":[{"name":"Laboratory of General Physiology, Department of Biology and Biotechnology \u201cLazzaro Spallanzani,\u201d University of Pavia, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7572-9504","authenticated-orcid":false,"given":"Elisa","family":"Rumi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"},{"name":"Department of Hematology Oncology, Foundation IRCCS Policlinico San Matteo, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6575-8766","authenticated-orcid":false,"given":"Daniela","family":"Pietra","sequence":"additional","affiliation":[{"name":"Department of Hematology Oncology, Foundation IRCCS Policlinico San Matteo, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo M.","family":"Soprano","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"},{"name":"Laboratory of Biochemistry, Biotechnology and Advanced Diagnosis, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo Foundation, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-2654","authenticated-orcid":false,"given":"Dmitry","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Universit\u00e0 del Piemonte Orientale, Novara, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-0812","authenticated-orcid":false,"given":"Alessandra","family":"Iurlo","sequence":"additional","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3304-8417","authenticated-orcid":false,"given":"Umberto","family":"Gianelli","sequence":"additional","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8357-7192","authenticated-orcid":false,"given":"Giovanni","family":"Barosi","sequence":"additional","affiliation":[{"name":"Center for the Study of Myelofibrosis, Foundation IRCCS Policlinico San Matteo, Pavia, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4195-2289","authenticated-orcid":false,"given":"Vittorio","family":"Rosti","sequence":"additional","affiliation":[{"name":"Center for the Study of Myelofibrosis, Foundation IRCCS Policlinico San Matteo, Pavia, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5915-6910","authenticated-orcid":false,"given":"Isabelle","family":"Plo","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche (UMR) 1170, Gustave Roussy, Universit\u00e9 Paris-Sud, INSERM, Villejuif, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6984-8817","authenticated-orcid":false,"given":"Mario","family":"Cazzola","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3145-1245","authenticated-orcid":false,"given":"Alessandra","family":"Balduini","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, Pavia, Italy;"},{"name":"Laboratory of Biochemistry, Biotechnology and Advanced Diagnosis, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo Foundation, Pavia, Italy;"},{"name":"Department of Biomedical Engineering, Tufts University, Medford, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"issue":"6","key":"2020010911592393200_B1","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1182\/blood-2016-10-695957","article-title":"Diagnosis, risk stratification, and response evaluation in classical myeloproliferative neoplasms","volume":"129","author":"Rumi","year":"2017","journal-title":"Blood"},{"issue":"1","key":"2020010911592393200_B2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/ajh.24221","article-title":"Myeloproliferative neoplasms: a decade of discoveries and treatment advances","volume":"91","author":"Tefferi","year":"2016","journal-title":"Am J Hematol"},{"issue":"8","key":"2020010911592393200_B3","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1007\/s00018-014-1813-y","article-title":"The secret life of a megakaryocyte: emerging roles in bone marrow homeostasis control","volume":"72","author":"Malara","year":"2015","journal-title":"Cell Mol Life Sci"},{"issue":"25","key":"2020010911592393200_B4","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1056\/NEJMoa1311347","article-title":"Somatic mutations of calreticulin in myeloproliferative neoplasms","volume":"369","author":"Klampfl","year":"2013","journal-title":"N Engl J Med"},{"issue":"2","key":"2020010911592393200_B5","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/leu.2015.277","article-title":"Differential clinical effects of different mutation subtypes in CALR-mutant myeloproliferative neoplasms","volume":"30","author":"Pietra","year":"2016","journal-title":"Leukemia"},{"issue":"10","key":"2020010911592393200_B6","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1182\/blood-2015-11-681932","article-title":"Thrombopoietin receptor activation by myeloproliferative neoplasm associated calreticulin mutants","volume":"127","author":"Chachoua","year":"2016","journal-title":"Blood"},{"issue":"10","key":"2020010911592393200_B7","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1182\/blood-2015-11-679571","article-title":"Calreticulin mutants in mice induce an MPL-dependent thrombocytosis with frequent progression to myelofibrosis","volume":"127","author":"Marty","year":"2016","journal-title":"Blood"},{"issue":"4","key":"2020010911592393200_B8","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1158\/2159-8290.CD-15-1434","article-title":"Mutant calreticulin requires both its mutant c-terminus and the thrombopoietin receptor for oncogenic transformation","volume":"6","author":"Elf","year":"2016","journal-title":"Cancer Discov"},{"key":"2020010911592393200_B9","article-title":"Mutant calreticulin interacts with MPL in the secretion pathway for activation on the cell surface [published online ahead of print 28 August 2019]","author":"Masubuchi","journal-title":"Leukemia"},{"issue":"12","key":"2020010911592393200_B10","doi-asserted-by":"crossref","DOI":"10.3390\/ijms17122055","article-title":"Pathophysiological significance of store-operated calcium entry in megakaryocyte function: opening new paths for understanding the role of calcium in thrombopoiesis","volume":"17","author":"Di Buduo","year":"2016","journal-title":"Int J Mol Sci"},{"issue":"4","key":"2020010911592393200_B11","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1083\/jcb.130.4.847","article-title":"Overexpression of calreticulin increases the Ca2+ capacity of rapidly exchanging Ca2+ stores and reveals aspects of their lumenal microenvironment and function","volume":"130","author":"Bastianutto","year":"1995","journal-title":"J Cell Biol"},{"issue":"6","key":"2020010911592393200_B12","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1091\/mbc.9.6.1513","article-title":"Delayed activation of the store-operated calcium current induced by calreticulin overexpression in RBL-1 cells","volume":"9","author":"Fasolato","year":"1998","journal-title":"Mol Biol Cell"},{"issue":"47","key":"2020010911592393200_B13","doi-asserted-by":"crossref","first-page":"36676","DOI":"10.1074\/jbc.M002041200","article-title":"Calreticulin modulates capacitative Ca2+ influx by controlling the extent of inositol 1,4,5-trisphosphate-induced Ca2+ store depletion","volume":"275","author":"Xu","year":"2000","journal-title":"J Biol Chem"},{"issue":"2","key":"2020010911592393200_B14","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/j.1538-7836.2011.04593.x","article-title":"The platelet-surface thiol isomerase enzyme ERp57 modulates platelet function","volume":"10","author":"Holbrook","year":"2012","journal-title":"J Thromb Haemost"},{"issue":"6","key":"2020010911592393200_B15","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1161\/ATVBAHA.116.307461","article-title":"Intracellular trafficking, localization, and mobilization of platelet-borne thiol isomerases","volume":"36","author":"Crescente","year":"2016","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"11","key":"2020010911592393200_B16","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1038\/embor.2011.173","article-title":"Modulation of STIM1 and capacitative Ca2+ entry by the endoplasmic reticulum luminal oxidoreductase ERp57","volume":"12","author":"Prins","year":"2011","journal-title":"EMBO Rep"},{"issue":"7","key":"2020010911592393200_B17","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1182\/blood-2017-08-800896","article-title":"Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN","volume":"131","author":"Elf","year":"2018","journal-title":"Blood"},{"issue":"22","key":"2020010911592393200_B18","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.122703","article-title":"Targeting the CALR interactome in myeloproliferative neoplasms","volume":"3","author":"Pronier","year":"2018","journal-title":"JCI Insight"},{"key":"2020010911592393200_B19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/978-1-4939-8585-2_11","article-title":"Three-dimensional tissue models for studying ex vivo megakaryocytopoiesis and platelet production","volume":"1812","author":"Di Buduo","year":"2018","journal-title":"Methods Mol Biol"},{"issue":"4","key":"2020010911592393200_B20","doi-asserted-by":"crossref","first-page":"769","DOI":"10.3324\/haematol.2013.096859","article-title":"The importance of calcium in the regulation of megakaryocyte function","volume":"99","author":"Di Buduo","year":"2014","journal-title":"Haematologica"},{"issue":"7","key":"2020010911592393200_B21","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.3324\/haematol.2016.161562","article-title":"A new path to platelet production through matrix sensing","volume":"102","author":"Abbonante","year":"2017","journal-title":"Haematologica"},{"issue":"9","key":"2020010911592393200_B22","doi-asserted-by":"crossref","first-page":"4343","DOI":"10.1074\/jbc.M115.674507","article-title":"Recombinant collagen engineered to bind to discoidin domain receptor functions as a receptor inhibitor","volume":"291","author":"An","year":"2016","journal-title":"J Biol Chem"},{"issue":"1","key":"2020010911592393200_B23","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/bjh.13471","article-title":"Altered fibronectin expression and deposition by myeloproliferative neoplasm-derived mesenchymal stromal cells","volume":"172","author":"Abbonante","year":"2016","journal-title":"Br J Haematol"},{"issue":"4","key":"2020010911592393200_B24","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1002\/stem.2285","article-title":"Thrombopoietin\/TGF-\u03b21 loop regulates megakaryocyte extracellular matrix component synthesis","volume":"34","author":"Abbonante","year":"2016","journal-title":"Stem Cells"},{"issue":"12","key":"2020010911592393200_B25","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.3324\/haematol.2016.146746","article-title":"Revealing eltrombopag\u2019s promotion of human megakaryopoiesis through AKT\/ERK-dependent pathway activation","volume":"101","author":"Di Buduo","year":"2016","journal-title":"Haematologica"},{"issue":"pt 11","key":"2020010911592393200_B26","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1113\/jphysiol.2009.172254","article-title":"TRPC1 and STIM1 mediate capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells","volume":"587","author":"Ng","year":"2009","journal-title":"J Physiol"},{"issue":"3","key":"2020010911592393200_B27","doi-asserted-by":"crossref","first-page":"C779","DOI":"10.1152\/ajpcell.00173.2008","article-title":"Ca2+ handling is altered when arterial myocytes progress from a contractile to a proliferative phenotype in culture","volume":"295","author":"Berra-Romani","year":"2008","journal-title":"Am J Physiol Cell Physiol"},{"key":"2020010911592393200_B28","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/978-1-61779-307-3_20","article-title":"Megakaryopoiesis and thrombopoiesis: an update on cytokines and lineage surface markers","volume":"788","author":"Yu","year":"2012","journal-title":"Methods Mol Biol"},{"issue":"10","key":"2020010911592393200_B29","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/j.exphem.2009.07.006","article-title":"Critical role for ERK1\/2 in bone marrow and fetal liver-derived primary megakaryocyte differentiation, motility, and proplatelet formation","volume":"37","author":"Mazharian","year":"2009","journal-title":"Exp Hematol"},{"issue":"37","key":"2020010911592393200_B30","doi-asserted-by":"crossref","first-page":"23366","DOI":"10.1074\/jbc.272.37.23366","article-title":"Sustained activation of the extracellular signal-regulated kinase\/mitogen-activated protein kinase pathway is required for megakaryocytic differentiation of K562 cells","volume":"272","author":"Racke","year":"1997","journal-title":"J Biol Chem"},{"issue":"9","key":"2020010911592393200_B31","doi-asserted-by":"crossref","first-page":"4991","DOI":"10.1128\/MCB.17.9.4991","article-title":"Control of thrombopoietin-induced megakaryocytic differentiation by the mitogen-activated protein kinase pathway","volume":"17","author":"Rouyez","year":"1997","journal-title":"Mol Cell Biol"},{"issue":"4","key":"2020010911592393200_B32","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1128\/MCB.17.4.1947","article-title":"Megakaryocytic differentiation induced by constitutive activation of mitogen-activated protein kinase kinase","volume":"17","author":"Whalen","year":"1997","journal-title":"Mol Cell Biol"},{"issue":"5","key":"2020010911592393200_B33","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1182\/blood.V94.5.1601","article-title":"Megakaryocyte growth and development factor-induced proliferation and differentiation are regulated by the mitogen-activated protein kinase pathway in primitive cord blood hematopoietic progenitors","volume":"94","author":"Fichelson","year":"1999","journal-title":"Blood"},{"issue":"11","key":"2020010911592393200_B34","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1016\/j.cellsig.2015.08.006","article-title":"Calcium signaling and cell proliferation","volume":"27","author":"Pinto","year":"2015","journal-title":"Cell Signal"},{"issue":"11","key":"2020010911592393200_B35","doi-asserted-by":"crossref","first-page":"4552","DOI":"10.1182\/blood.V87.11.4552.bloodjournal87114552","article-title":"Establishment and characterization of the thrombopoietin-dependent megakaryocytic cell line, UT-7\/TPO","volume":"87","author":"Komatsu","year":"1996","journal-title":"Blood"},{"issue":"2","key":"2020010911592393200_B36","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1006\/bbrc.1996.5969","article-title":"Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction","volume":"231","author":"Kunitama","year":"1997","journal-title":"Biochem Biophys Res Commun"},{"issue":"6","key":"2020010911592393200_B37","doi-asserted-by":"crossref","first-page":"C1667","DOI":"10.1152\/ajpcell.00265.2004","article-title":"Erythropoietin-modulated calcium influx through TRPC2 is mediated by phospholipase Cgamma and IP3R","volume":"287","author":"Tong","year":"2004","journal-title":"Am J Physiol Cell Physiol"},{"issue":"5","key":"2020010911592393200_B38","doi-asserted-by":"crossref","first-page":"C993","DOI":"10.1152\/ajpcell.00325.2009","article-title":"Essential role for STIM1\/Orai1-mediated calcium influx in PDGF-induced smooth muscle migration","volume":"298","author":"Bisaillon","year":"2010","journal-title":"Am J Physiol Cell Physiol"},{"issue":"3-4","key":"2020010911592393200_B39","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/BF03033983","article-title":"Role of the JAKs\/STATs pathway in the intracellular calcium changes induced by interleukin-6 in hippocampal neurons","volume":"8","author":"Orellana","year":"2005","journal-title":"Neurotox Res"},{"issue":"1","key":"2020010911592393200_B40","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1089\/scd.2017.0114","article-title":"Stromal cell-derived factor-1\u03b1 promotes endothelial colony-forming cell migration through the Ca2+-dependent activation of the extracellular signal-regulated kinase 1\/2 and phosphoinositide 3-kinase\/AKT pathways","volume":"27","author":"Zuccolo","year":"2018","journal-title":"Stem Cells Dev"},{"issue":"8","key":"2020010911592393200_B41","doi-asserted-by":"crossref","first-page":"3239","DOI":"10.1074\/jbc.M116.751693","article-title":"The plant hormone abscisic acid is a prosurvival factor in human and murine megakaryocytes","volume":"292","author":"Malara","year":"2017","journal-title":"J Biol Chem"},{"issue":"9","key":"2020010911592393200_B42","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1016\/j.cellsig.2015.05.004","article-title":"Inhibition of glutamate regulated calcium entry into leukemic megakaryoblasts reduces cell proliferation and supports differentiation","volume":"27","author":"Kamal","year":"2015","journal-title":"Cell Signal"},{"issue":"2","key":"2020010911592393200_B43","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/cbin.10558","article-title":"Increased expression of transient receptor potential canonical 6 (TRPC6) in differentiating human megakaryocytes","volume":"40","author":"Ramanathan","year":"2016","journal-title":"Cell Biol Int"},{"issue":"33","key":"2020010911592393200_B44","doi-asserted-by":"crossref","first-page":"29686","DOI":"10.1074\/jbc.M202405200","article-title":"Localization of the lectin, ERp57 binding, and polypeptide binding sites of calnexin and calreticulin","volume":"277","author":"Leach","year":"2002","journal-title":"J Biol Chem"},{"issue":"4","key":"2020010911592393200_B45","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1073\/pnas.042699099","article-title":"TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain","volume":"99","author":"Frickel","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"issue":"10","key":"2020010911592393200_B46","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1182\/blood-2015-09-671172","article-title":"Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms","volume":"127","author":"Araki","year":"2016","journal-title":"Blood"},{"issue":"1","key":"2020010911592393200_B47","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/s41375-018-0181-2","article-title":"Homomultimerization of mutant calreticulin is a prerequisite for MPL binding and activation","volume":"33","author":"Araki","year":"2019","journal-title":"Leukemia"},{"key":"2020010911592393200_B48","doi-asserted-by":"crossref","first-page":"102061","DOI":"10.1016\/j.ceca.2019.102061","article-title":"ER Ca2+ release and store-operated Ca2+ entry - partners in crime or independent actors in oncogenic transformation?","volume":"82","author":"Pierro","year":"2019","journal-title":"Cell Calcium"},{"issue":"7","key":"2020010911592393200_B49","doi-asserted-by":"crossref","DOI":"10.3390\/cancers11070899","article-title":"Store-operated Ca2+ entry in tumor progression: from molecular mechanisms to clinical implications","volume":"11","author":"Chen","year":"2019","journal-title":"Cancers (Basel)"},{"issue":"9","key":"2020010911592393200_B50","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1002\/ijc.29840","article-title":"SOCE and cancer: recent progress and new perspectives","volume":"138","author":"Xie","year":"2016","journal-title":"Int J Cancer"},{"issue":"1","key":"2020010911592393200_B51","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s40425-019-0591-3","article-title":"STIM1 at the plasma membrane as a new target in progressive chronic lymphocytic leukemia","volume":"7","author":"Debant","year":"2019","journal-title":"J Immunother Cancer"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/135\/2\/133\/1551013\/bloodbld2019001103.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/135\/2\/133\/1551013\/bloodbld2019001103.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T12:07:11Z","timestamp":1578571631000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/135\/2\/133\/422538\/Defective-interaction-of-mutant-calreticulin-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,9]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,1,9]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2019001103","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,9]]}}}