{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:01:22Z","timestamp":1790521282447,"version":"4.1.0"},"reference-count":46,"publisher":"American Society of Hematology","issue":"24","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Although human B cells have been extensively studied, most reports have used peripheral blood as a source. Here, we used a unique tissue resource derived from healthy organ donors to deeply characterize human B-cell compartments across multiple tissues and donors. These datasets revealed that B cells in the blood are not in homeostasis with compartments in other tissues. We found striking donor-to-donor variability in the frequencies and isotype of CD27+ memory B cells (MBCs). A comprehensive antibody-based screen revealed markers of MBC and allowed identification of novel MBC subsets with distinct functions defined according to surface expression of CD69 and CD45RB. We defined a tissue-resident MBC phenotype that was predominant in the gut but absent in blood. RNA-sequencing of MBC subsets from multiple tissues revealed a tissue-resident MBC gene signature as well as gut- and spleen-specific signatures. Overall, these studies provide novel insights into the nature and function of human B-cell compartments across multiple tissues.<\/jats:p>","DOI":"10.1182\/blood.2019002782","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T16:03:39Z","timestamp":1596557019000},"page":"2774-2785","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":126,"title":["Comprehensive analyses of B-cell compartments across the human body reveal novel subsets and a gut-resident memory phenotype"],"prefix":"10.1182","volume":"136","author":[{"given":"Nadine M.","family":"Weisel","sequence":"first","affiliation":[{"name":"Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8679-9241","authenticated-orcid":false,"given":"Florian 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