{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:21:20Z","timestamp":1790824880843,"version":"4.1.0"},"reference-count":75,"publisher":"American Society of Hematology","issue":"11","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,9,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>There is an urgent need to understand the pathogenesis of coronavirus disease 2019 (COVID-19). In particular, thrombotic complications in patients with COVID-19 are common and contribute to organ failure and mortality. Patients with severe COVID-19 present with hemostatic abnormalities that mimic disseminated intravascular coagulopathy associated with sepsis, with the major difference being increased risk of thrombosis rather than bleeding. However, whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection alters platelet function to contribute to the pathophysiology of COVID-19 remains unknown. In this study, we report altered platelet gene expression and functional responses in patients infected with SARS-CoV-2. RNA sequencing demonstrated distinct changes in the gene-expression profile of circulating platelets of COVID-19 patients. Pathway analysis revealed differential gene-expression changes in pathways associated with protein ubiquitination, antigen presentation, and mitochondrial dysfunction. The receptor for SARS-CoV-2 binding, angiotensin-converting enzyme 2 (ACE2), was not detected by messenger RNA (mRNA) or protein in platelets. Surprisingly, mRNA from the SARS-CoV-2 N1 gene was detected in platelets from 2 of 25 COVID-19 patients, suggesting that platelets may take-up SARS-COV-2 mRNA independent of ACE2. Resting platelets from COVID-19 patients had increased P-selectin expression basally and upon activation. Circulating platelet-neutrophil, -monocyte, and -T-cell aggregates were all significantly elevated in COVID-19 patients compared with healthy donors. Furthermore, platelets from COVID-19 patients aggregated faster and showed increased spreading on both fibrinogen and collagen. The increase in platelet activation and aggregation could partially be attributed to increased MAPK pathway activation and thromboxane generation. These findings demonstrate that SARS-CoV-2 infection is associated with platelet hyperreactivity, which may contribute to COVID-19 pathophysiology.<\/jats:p>","DOI":"10.1182\/blood.2020007214","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T10:02:33Z","timestamp":1592906553000},"page":"1317-1329","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":872,"title":["Platelet gene expression and function in patients with COVID-19"],"prefix":"10.1182","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6848-4484","authenticated-orcid":false,"given":"Bhanu Kanth","family":"Manne","sequence":"first","affiliation":[{"name":"Molecular Medicine Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-3568","authenticated-orcid":false,"given":"Frederik","family":"Denorme","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Middleton","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Internal Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Portier","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse W.","family":"Rowley","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Internal Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Stubben","sequence":"additional","affiliation":[{"name":"Bioinformatics Shared Resource, Huntsman Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron C.","family":"Petrey","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Pathology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal D.","family":"Tolley","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Guo","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Cody","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Pediatrics, University of Utah, Salt Lake City, UT; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Weyrich","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Internal Medicine,"},{"name":"Department of Pathology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian C.","family":"Yost","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Pediatrics, University of Utah, Salt Lake City, UT; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Rondina","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Internal Medicine,"},{"name":"Department of Pathology, and"},{"name":"Department of Internal Medicine, George E. Wahlen Department of Veterans Affairs (VA) Medical Center, and"},{"name":"Geriatric Research, Education, and Clinical Center (GRECC), VA Salt Lake City Healthcare System, Salt Lake City, UT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0027-694X","authenticated-orcid":false,"given":"Robert A.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program,"},{"name":"Department of Internal Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"issue":"23","key":"2020091011283971000_B1","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1182\/blood.2020006000","article-title":"COVID-19 and its implications for thrombosis and anticoagulation","volume":"135","author":"Connors","year":"2020","journal-title":"Blood"},{"key":"2020091011283971000_B2","article-title":"Pulmonary arterial thrombosis in COVID-19 with fatal outcome: results from a prospective, single-center, clinicopathologic case series [published online ahead of print 14 May 2020]","author":"Lax","journal-title":"Ann Intern Med"},{"issue":"7","key":"2020091011283971000_B3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41574-020-0364-6","article-title":"Obesity and impaired metabolic health in patients with COVID-19","volume":"16","author":"Stefan","year":"2020","journal-title":"Nat Rev Endocrinol"},{"key":"2020091011283971000_B4","article-title":"Pulmonary embolism in patients with COVID-19: awareness of an increased prevalence","author":"Poissy","journal-title":"Circulation"},{"key":"2020091011283971000_B5","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.3389\/fimmu.2019.02204","article-title":"The era of thromboinflammation: platelets are dynamic sensors and effector cells during infectious diseases","volume":"10","author":"Guo","year":"2019","journal-title":"Front Immunol"},{"issue":"4","key":"2020091011283971000_B6","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1152\/physrev.00038.2015","article-title":"Platelets in pulmonary immune responses and inflammatory lung diseases","volume":"96","author":"Middleton","year":"2016","journal-title":"Physiol Rev"},{"issue":"2","key":"2020091011283971000_B7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1161\/CIRCRESAHA.117.310795","article-title":"Circulating platelets as mediators of immunity, inflammation, and thrombosis","volume":"122","author":"Koupenova","year":"2018","journal-title":"Circ Res"},{"issue":"4","key":"2020091011283971000_B8","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nri2956","article-title":"Platelets and the immune continuum","volume":"11","author":"Semple","year":"2011","journal-title":"Nat Rev Immunol"},{"issue":"12","key":"2020091011283971000_B9","doi-asserted-by":"crossref","first-page":"5579","DOI":"10.4049\/jimmunol.1500259","article-title":"Nouvelle cuisine: platelets served with inflammation","volume":"194","author":"Kapur","year":"2015","journal-title":"J Immunol"},{"issue":"20","key":"2020091011283971000_B10","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1182\/bloodadvances.2017007021","article-title":"Mature murine megakaryocytes present antigen-MHC class I molecules to T cells and transfer them to platelets","volume":"1","author":"Zufferey","year":"2017","journal-title":"Blood Adv"},{"issue":"18","key":"2020091011283971000_B11","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1182\/blood-2013-07-515536","article-title":"Influenza virus H1N1 activates platelets through Fc\u03b3RIIA signaling and thrombin generation","volume":"123","author":"Boilard","year":"2014","journal-title":"Blood"},{"issue":"19","key":"2020091011283971000_B12","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1182\/blood-2018-09-873984","article-title":"Human megakaryocytes possess intrinsic antiviral immunity through regulated induction of IFITM3","volume":"133","author":"Campbell","year":"2019","journal-title":"Blood"},{"issue":"4","key":"2020091011283971000_B13","doi-asserted-by":"crossref","first-page":"e1007625","DOI":"10.1371\/journal.ppat.1007625","article-title":"Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage","volume":"15","author":"Chao","year":"2019","journal-title":"PLoS Pathog"},{"issue":"1","key":"2020091011283971000_B14","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1038\/s41467-019-09607-x","article-title":"The role of platelets in mediating a response to human influenza infection","volume":"10","author":"Koupenova","year":"2019","journal-title":"Nat Commun"},{"issue":"1","key":"2020091011283971000_B15","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1038\/s41467-019-10360-4","article-title":"Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A\/TLR2","volume":"10","author":"Sung","year":"2019","journal-title":"Nat Commun"},{"issue":"24","key":"2020091011283971000_B16","doi-asserted-by":"crossref","first-page":"9569","DOI":"10.1073\/pnas.1207314109","article-title":"Identification of the platelet-derived chemokine CXCL4\/PF-4 as a broad-spectrum HIV-1 inhibitor","volume":"109","author":"Auerbach","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"issue":"11","key":"2020091011283971000_B17","doi-asserted-by":"crossref","first-page":"e1002355","DOI":"10.1371\/journal.ppat.1002355","article-title":"Novel anti-bacterial activities of \u03b2-defensin 1 in human platelets: suppression of pathogen growth and signaling of neutrophil extracellular trap formation","volume":"7","author":"Kraemer","year":"2011","journal-title":"PLoS Pathog"},{"issue":"12","key":"2020091011283971000_B18","doi-asserted-by":"crossref","first-page":"6524","DOI":"10.1128\/IAI.70.12.6524-6533.2002","article-title":"Antimicrobial peptides from human platelets","volume":"70","author":"Tang","year":"2002","journal-title":"Infect Immun"},{"issue":"3","key":"2020091011283971000_B19","first-page":"449","article-title":"The role of platelets in inflammation","volume":"114","author":"Thomas","year":"2015","journal-title":"Thromb Haemost"},{"issue":"1","key":"2020091011283971000_B20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri3345","article-title":"Thrombosis as an intravascular effector of innate immunity","volume":"13","author":"Engelmann","year":"2013","journal-title":"Nat Rev Immunol"},{"issue":"12","key":"2020091011283971000_B21","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1182\/blood.2019000067","article-title":"Sepsis alters the transcriptional and translational landscape of human and murine platelets","volume":"134","author":"Middleton","year":"2019","journal-title":"Blood"},{"issue":"4","key":"2020091011283971000_B22","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1161\/CIRCRESAHA.119.315215","article-title":"Longitudinal RNA-Seq analysis of the repeatability of gene expression and splicing in human platelets identifies a platelet SELP splice QTL","volume":"126","author":"Rondina","year":"2020","journal-title":"Circ Res"},{"issue":"1","key":"2020091011283971000_B23","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4049\/jimmunol.1700885","article-title":"Granzyme A in human platelets regulates the synthesis of proinflammatory cytokines by monocytes in aging","volume":"200","author":"Campbell","year":"2018","journal-title":"J Immunol"},{"issue":"10","key":"2020091011283971000_B24","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1161\/ATVBAHA.117.309794","article-title":"Clots are potent triggers of inflammatory cell gene expression: indications for timely fibrinolysis","volume":"37","author":"Campbell","year":"2017","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"2020091011283971000_B25","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/j.1538-7836.2011.04208.x","article-title":"The septic milieu triggers expression of spliced tissue factor mRNA in human platelets","volume":"9","author":"Rondina","year":"2011","journal-title":"J Thromb Haemost"},{"issue":"6","key":"2020091011283971000_B26","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1378\/chest.11-2860","article-title":"In vivo platelet activation in critically ill patients with primary 2009 influenza A(H1N1)","volume":"141","author":"Rondina","year":"2012","journal-title":"Chest"},{"issue":"5","key":"2020091011283971000_B27","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1111\/jth.14748","article-title":"Phospho-inositide-dependent kinase 1 regulates signal dependent translation in megakaryocytes and platelets","volume":"18","author":"Manne","year":"2020","journal-title":"J Thromb Haemost"},{"issue":"6","key":"2020091011283971000_B28","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1111\/jth.14005","article-title":"PDK1 governs thromboxane generation and thrombosis in platelets by regulating activation of Raf1 in the MAPK pathway","volume":"16","author":"Manne","year":"2018","journal-title":"J Thromb Haemost"},{"key":"2020091011283971000_B29","article-title":"Pulmonary embolism and increased levels of d-dimer in patients with coronavirus disease","author":"Griffin","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"2020091011283971000_B30","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/infdis\/jiaa224","article-title":"Interferon-induced transmembrane protein-3 genetic variant rs12252-C is associated with disease severity in COVID-19","volume":"222","author":"Zhang","year":"2020","journal-title":"J Infect Dis"},{"issue":"6","key":"2020091011283971000_B31","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1055\/s-0040-1710018","article-title":"COVID-19-related severe hypercoagulability in patients admitted to intensive care unit for acute respiratory failure","volume":"120","author":"Spiezia","year":"2020","journal-title":"Thromb Haemost"},{"issue":"6","key":"2020091011283971000_B32","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1111\/jth.14848","article-title":"Thrombocytopenia and its association with mortality in patients with COVID-19","volume":"18","author":"Yang","year":"2020","journal-title":"J Thromb Haemost"},{"key":"2020091011283971000_B33","doi-asserted-by":"crossref","first-page":"104362","DOI":"10.1016\/j.jcv.2020.104362","article-title":"Coagulation disorders in coronavirus infected patients: COVID-19, SARS-CoV-1, MERS-CoV and lessons from the past","volume":"127","author":"Giannis","year":"2020","journal-title":"J Clin Virol"},{"issue":"7","key":"2020091011283971000_B34","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1161\/01.ATV.0000167524.69092.16","article-title":"Platelet-leukocyte interaction and platelet activation in acute stroke with and without preceding infection","volume":"25","author":"Zeller","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"2020091011283971000_B35","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1007\/s11239-015-1212-8","article-title":"Platelet leukocyte aggregates and markers of platelet aggregation, immune activation and disease progression in HIV infected treatment naive asymptomatic individuals","volume":"40","author":"Nkambule","year":"2015","journal-title":"J Thromb Thrombolysis"},{"issue":"6","key":"2020091011283971000_B36","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1182\/blood.V53.6.1043.1043","article-title":"Platelet alpha granules contain a growth factor for fibroblasts","volume":"53","author":"Kaplan","year":"1979","journal-title":"Blood"},{"issue":"4","key":"2020091011283971000_B37","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.blre.2009.04.001","article-title":"Platelet alpha-granules: basic biology and clinical correlates","volume":"23","author":"Blair","year":"2009","journal-title":"Blood Rev"},{"issue":"6","key":"2020091011283971000_B38","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1007\/s00134-020-06062-x","article-title":"High risk of thrombosis in patients with severe SARS-CoV-2 infection: a multicenter prospective cohort study","volume":"46","author":"Helms","year":"2020","journal-title":"Intensive Care Med"},{"key":"2020091011283971000_B39","article-title":"High incidence of venous thromboembolic events in anticoagulated severe COVID-19 patients [published online ahead of print 22 April 2020]","author":"Llitjos","journal-title":"J Thromb Haemost"},{"issue":"6","key":"2020091011283971000_B40","doi-asserted-by":"crossref","first-page":"E131","DOI":"10.1002\/ajh.25774","article-title":"Hematologic parameters in patients with COVID-19 infection","volume":"95","author":"Fan","year":"2020","journal-title":"Am J Hematol"},{"key":"2020091011283971000_B41","article-title":"Characteristics of ischaemic stroke associated with COVID-19","author":"Beyrouti","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"2020091011283971000_B42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.thromres.2020.04.013","article-title":"Incidence of thrombotic complications in critically ill ICU patients with COVID-19","volume":"191","author":"Klok","year":"2020","journal-title":"Thromb Res"},{"key":"2020091011283971000_B43","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.thromres.2020.04.024","article-title":"Venous and arterial thromboembolic complications in COVID-19 patients admitted to an academic hospital in Milan, Italy","volume":"191","author":"Lodigiani","year":"2020","journal-title":"Thromb Res"},{"issue":"7395","key":"2020091011283971000_B44","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nature10921","article-title":"IFITM3 restricts the morbidity and mortality associated with influenza","volume":"484","author":"Everitt","year":"2012","journal-title":"Nature"},{"issue":"4","key":"2020091011283971000_B45","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1083\/jcb.201111136","article-title":"T granules in human platelets function in TLR9 organization and signaling","volume":"198","author":"Thon","year":"2012","journal-title":"J Cell Biol"},{"issue":"1","key":"2020091011283971000_B46","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1161\/CIRCRESAHA.112.274241","article-title":"Engagement of platelet toll-like receptor 9 by novel endogenous ligands promotes platelet hyperreactivity and thrombosis","volume":"112","author":"Panigrahi","year":"2013","journal-title":"Circ Res"},{"issue":"5","key":"2020091011283971000_B47","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1182\/blood-2013-11-536003","article-title":"Platelet-TLR7 mediates host survival and platelet count during viral infection in the absence of platelet-dependent thrombosis","volume":"124","author":"Koupenova","year":"2014","journal-title":"Blood"},{"issue":"7","key":"2020091011283971000_B48","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1161\/ATVBAHA.120.314180","article-title":"Platelets endocytose viral particles and are activated via TLR (Toll-like receptor) signaling","volume":"40","author":"Banerjee","year":"2020","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"2020091011283971000_B49","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1080\/09537104.2020.1754383","article-title":"Association between platelet parameters and mortality in coronavirus disease 2019: retrospective cohort study","volume":"31","author":"Liu","year":"2020","journal-title":"Platelets"},{"issue":"7","key":"2020091011283971000_B50","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1055\/s-0038-1657778","article-title":"Thrombocytopenia and platelet dysfunction in acute tropical infectious diseases","volume":"44","author":"Hapsari Putri","year":"2018","journal-title":"Semin Thromb Hemost"},{"key":"2020091011283971000_B51","first-page":"w13788","article-title":"Thrombocytopenia as a mortality risk factor in acute respiratory failure in H1N1 influenza","volume":"143","author":"Lopez-Delgado","year":"2013","journal-title":"Swiss Med Wkly"},{"key":"2020091011283971000_B52","doi-asserted-by":"crossref","first-page":"41697","DOI":"10.1038\/srep41697","article-title":"Platelet activation determines the severity of thrombocytopenia in dengue infection","volume":"7","author":"Ojha","year":"2017","journal-title":"Sci Rep"},{"issue":"4","key":"2020091011283971000_B53","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.thromres.2007.12.021","article-title":"Thrombopoietin levels increased in patients with severe acute respiratory syndrome","volume":"122","author":"Yang","year":"2008","journal-title":"Thromb Res"},{"key":"2020091011283971000_B54","doi-asserted-by":"crossref","first-page":"649","DOI":"10.3389\/fimmu.2014.00649","article-title":"Platelets and infection - an emerging role of platelets in viral infection","volume":"5","author":"Assinger","year":"2014","journal-title":"Front Immunol"},{"issue":"5","key":"2020091011283971000_B55","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1097\/QAI.0000000000000048","article-title":"TGF\u03b2-mediated downregulation of thrombopoietin is associated with platelet decline in asymptomatic SIV infection","volume":"65","author":"Metcalf Pate","year":"2014","journal-title":"J Acquir Immune Defic Syndr"},{"issue":"3","key":"2020091011283971000_B56","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/j.1365-2141.2000.02430.x","article-title":"The platelet thrombopoietin receptor number and function are markedly decreased in patients with essential thrombocythaemia","volume":"111","author":"Li","year":"2000","journal-title":"Br J Haematol"},{"issue":"10","key":"2020091011283971000_B57","doi-asserted-by":"crossref","first-page":"4031","DOI":"10.1182\/blood.V90.10.4031","article-title":"Markedly reduced expression of platelet c-mpl receptor in essential thrombocythemia","volume":"90","author":"Horikawa","year":"1997","journal-title":"Blood"},{"issue":"6","key":"2020091011283971000_B58","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1182\/blood.V87.6.2154.bloodjournal8762154","article-title":"Regulation of thrombopoietin levels by c-mpl-mediated binding to platelets","volume":"87","author":"Fielder","year":"1996","journal-title":"Blood"},{"issue":"1","key":"2020091011283971000_B59","doi-asserted-by":"crossref","first-page":"14999","DOI":"10.1038\/s41598-018-33403-0","article-title":"Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals","volume":"8","author":"Mesquita","year":"2018","journal-title":"Sci Rep"},{"issue":"1","key":"2020091011283971000_B60","doi-asserted-by":"crossref","first-page":"4978","DOI":"10.1038\/s41598-017-04773-8","article-title":"Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis [published correction appears in Sci Rep. 2018;8(1):6224]","volume":"7","author":"Ma","year":"2017","journal-title":"Sci Rep"},{"issue":"7","key":"2020091011283971000_B61","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1164\/rccm.201406-1031OC","article-title":"Platelet activation and aggregation promote lung inflammation and influenza virus pathogenesis","volume":"191","author":"L\u00ea","year":"2015","journal-title":"Am J Respir Crit Care Med"},{"issue":"11","key":"2020091011283971000_B62","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1084\/jem.20021868","article-title":"Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin","volume":"197","author":"Falati","year":"2003","journal-title":"J Exp Med"},{"issue":"7","key":"2020091011283971000_B63","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1172\/JCI61303","article-title":"Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury","volume":"122","author":"Caudrillier","year":"2012","journal-title":"J Clin Invest"},{"issue":"409","key":"2020091011283971000_B64","doi-asserted-by":"crossref","first-page":"eaam5861","DOI":"10.1126\/scitranslmed.aam5861","article-title":"Neutrophil macroaggregates promote widespread pulmonary thrombosis after gut ischemia","volume":"9","author":"Yuan","year":"2017","journal-title":"Sci Transl Med"},{"issue":"12","key":"2020091011283971000_B65","doi-asserted-by":"crossref","first-page":"3211","DOI":"10.1172\/JCI29499","article-title":"Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation","volume":"116","author":"Zarbock","year":"2006","journal-title":"J Clin Invest"},{"issue":"9","key":"2020091011283971000_B66","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1161\/CIRCRESAHA.115.306847","article-title":"Platelets play differential role during the initiation and progression of autoimmune neuroinflammation","volume":"117","author":"Starossom","year":"2015","journal-title":"Circ Res"},{"issue":"3","key":"2020091011283971000_B67","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1189\/jlb.0213074","article-title":"Functional consequences of platelet binding to T lymphocytes in inflammation","volume":"94","author":"Zamora","year":"2013","journal-title":"J Leukoc Biol"},{"issue":"2","key":"2020091011283971000_B68","doi-asserted-by":"crossref","first-page":"916","DOI":"10.4049\/jimmunol.1200580","article-title":"Platelets present antigen in the context of MHC class I","volume":"189","author":"Chapman","year":"2012","journal-title":"J Immunol"},{"issue":"5","key":"2020091011283971000_B69","doi-asserted-by":"crossref","first-page":"e1006385","DOI":"10.1371\/journal.ppat.1006385","article-title":"Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue","volume":"13","author":"Trugilho","year":"2017","journal-title":"PLoS Pathog"},{"issue":"5","key":"2020091011283971000_B70","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1097\/QAD.0b013e328336098c","article-title":"Platelet function and HIV: a case-control study","volume":"24","author":"Satchell","year":"2010","journal-title":"AIDS"},{"key":"2020091011283971000_B71","doi-asserted-by":"crossref","first-page":"139437","DOI":"10.1172\/jci.insight.139437","article-title":"Intravascular hemolysis triggers ADP-mediated generation of platelet-rich thrombi in pre-capillary pulmonary arterioles","author":"Brzoska","year":"2020","journal-title":"JCI Insight"},{"issue":"4","key":"2020091011283971000_B72","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1182\/blood-2008-05-155978","article-title":"Two distinct roles of mitogen-activated protein kinases in platelets and a novel Rac1-MAPK-dependent integrin outside-in retractile signaling pathway","volume":"113","author":"Flevaris","year":"2009","journal-title":"Blood"},{"issue":"21","key":"2020091011283971000_B73","doi-asserted-by":"crossref","first-page":"17815","DOI":"10.1074\/jbc.M011405200","article-title":"Role of a JAK3-dependent biochemical signaling pathway in platelet activation and aggregation","volume":"276","author":"Tibbles","year":"2001","journal-title":"J Biol Chem"},{"issue":"7","key":"2020091011283971000_B74","doi-asserted-by":"crossref","first-page":"3897","DOI":"10.4049\/jimmunol.162.7.3897","article-title":"JAK3, STAT, and MAPK signaling pathways as novel molecular targets for the tyrphostin AG-490 regulation of IL-2-mediated T cell response","volume":"162","author":"Wang","year":"1999","journal-title":"J Immunol"},{"issue":"19","key":"2020091011283971000_B75","doi-asserted-by":"crossref","first-page":"13325","DOI":"10.1074\/jbc.M112.428359","article-title":"Dual mechanism of integrin \u03b1IIb\u03b23 closure in procoagulant platelets","volume":"288","author":"Mattheij","year":"2013","journal-title":"J Biol Chem"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/136\/11\/1317\/1757264\/bloodbld2020007214.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/136\/11\/1317\/1757264\/bloodbld2020007214.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T03:01:18Z","timestamp":1723086078000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/136\/11\/1317\/461106\/Platelet-gene-expression-and-function-in-patients"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,10]]},"references-count":75,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,9,10]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2020007214","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.738191212.793576153","asserted-by":"object"}]},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,10]]}}}