{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:51:09Z","timestamp":1790211069546,"version":"4.1.0"},"reference-count":25,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In hematopoietic cell transplantation (HCT), permissive HLA-DPB1 mismatches between patients and their unrelated donors are associated with improved outcomes compared with nonpermissive mismatches, but the underlying mechanism is incompletely understood. Here, we used mass spectrometry, T-cell receptor-\u03b2 (TCR\u03b2) deep sequencing, and cellular in vitro models of alloreactivity to interrogate the HLA-DP immunopeptidome and its role in alloreactive T-cell responses. We find that permissive HLA-DPB1 mismatches display significantly higher peptide repertoire overlaps compared with their nonpermissive counterparts, resulting in lower frequency and diversity of alloreactive TCR\u03b2 clonotypes in healthy individuals and transplanted patients. Permissiveness can be reversed by the absence of the peptide editor HLA-DM or the presence of its antagonist, HLA-DO, through significant broadening of the peptide repertoire. Our data establish the degree of immunopeptidome divergence between donor and recipient as the mechanistic basis for the clinically relevant permissive HLA-DPB1 mismatches in HCT and show that permissiveness is dependent on HLA-DM\u2013mediated peptide editing. Its key role for harnessing T-cell alloreactivity to HLA-DP highlights HLA-DM as a potential novel target for cellular and immunotherapy of leukemia.<\/jats:p>","DOI":"10.1182\/blood.2020008464","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T01:56:26Z","timestamp":1602035786000},"page":"923-928","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":63,"title":["Permissive HLA-DPB1 mismatches in HCT depend on immunopeptidome divergence and editing by HLA-DM"],"prefix":"10.1182","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4810-3653","authenticated-orcid":false,"given":"Thuja","family":"Meurer","sequence":"first","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9668-5013","authenticated-orcid":false,"given":"Pietro","family":"Crivello","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Metzing","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8960-7348","authenticated-orcid":false,"given":"Michel","family":"Kester","sequence":"additional","affiliation":[{"name":"Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1058-7744","authenticated-orcid":false,"given":"Dominik A.","family":"Megger","sequence":"additional","affiliation":[{"name":"Medical Proteome Center, Center for Protein Diagnostics, Ruhr University Bochum, Bochum, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Medical Proteome Center, Center for Protein Diagnostics, Ruhr University Bochum, Bochum, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7898-9408","authenticated-orcid":false,"given":"Peter A.","family":"van Veelen","sequence":"additional","affiliation":[{"name":"Centre for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-4664","authenticated-orcid":false,"given":"Peter","family":"van Balen","sequence":"additional","affiliation":[{"name":"Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2121-2892","authenticated-orcid":false,"given":"Astrid M.","family":"Westendorf","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6430-4802","authenticated-orcid":false,"given":"Georg","family":"Homa","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia E.","family":"Layer","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1347-3360","authenticated-orcid":false,"given":"Amin T.","family":"Turki","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cellular Therapy, University Hospital Essen, Essen, Germany;"},{"name":"Department of Bone Marrow Transplantation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3001-4441","authenticated-orcid":false,"given":"Marieke","family":"Griffioen","sequence":"additional","affiliation":[{"name":"Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Horn","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany; 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