{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T13:40:24Z","timestamp":1792935624402,"version":"4.5.0"},"reference-count":59,"publisher":"American Society of Hematology","issue":"1","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aplastic anemia (AA) is a T cell\u2013mediated autoimmune disorder of the hematopoietic system manifested by severe depletion of the hematopoietic stem and progenitor cells (HSPCs). Nonetheless, our understanding of the complex relationship between HSPCs and T cells is still obscure, mainly limited by techniques and the sparsity of HSPCs in the context of bone marrow failure. Here we performed single-cell transcriptome analysis of residual HSPCs and T cells to identify the molecular players from patients with AA. We observed that residual HSPCs in AA exhibited lineage-specific alterations in gene expression and transcriptional regulatory networks, indicating a selective disruption of distinct lineage-committed progenitor pools. In particular, HSPCs displayed frequently altered alternative splicing events and skewed patterns of polyadenylation in transcripts related to DNA damage and repair, suggesting a likely role in AA progression to myelodysplastic syndromes. We further identified cell type\u2013specific ligand-receptor interactions as potential mediators for ongoing HSPCs destruction by T cells. By tracking patients after immunosuppressive therapy (IST), we showed that hematopoiesis remission was incomplete accompanied by IST insensitive interactions between HSPCs and T cells as well as sustained abnormal transcription state. These data collectively constitute the transcriptomic landscape of disrupted hematopoiesis in AA at single-cell resolution, providing new insights into the molecular interactions of engaged T cells with residual HSPCs and render novel therapeutic opportunities for AA.<\/jats:p>","DOI":"10.1182\/blood.2020008966","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T00:55:54Z","timestamp":1616633754000},"page":"23-33","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":78,"title":["Single-cell transcriptomics dissects hematopoietic cell destruction and T-cell engagement in aplastic anemia"],"prefix":"10.1182","volume":"138","author":[{"given":"Caiying","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"},{"name":"Regenerative Medicine Clinic, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences\u2013Peking Union Medical College, Tianjin, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenchen","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin\u2019s Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibin","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"},{"name":"Regenerative Medicine Clinic, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences\u2013Peking Union Medical College, Tianjin, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanlan","family":"Ai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"},{"name":"Regenerative Medicine Clinic, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences\u2013Peking Union Medical College, Tianjin, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"},{"name":"Regenerative Medicine Clinic, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences\u2013Peking Union Medical College, Tianjin, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"},{"name":"Regenerative Medicine Clinic, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences\u2013Peking Union Medical College, Tianjin, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4521-7524","authenticated-orcid":false,"given":"Ping","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital,"},{"name":"Center for Stem Cell Medicine and Department of Stem Cell & Regenerative Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","published-online":{"date-parts":[[2021,3,24]]},"reference":[{"issue":"6","key":"2021070815251428300_B1","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1182\/blood.V88.6.1983.bloodjournal8861983","article-title":"A severe and consistent deficit in marrow and circulating primitive hematopoietic cells (long-term culture-initiating cells) in acquired aplastic anemia","volume":"88","author":"Maciejewski","year":"1996","journal-title":"Blood"},{"issue":"17","key":"2021070815251428300_B2","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1056\/NEJMra1413485","article-title":"Aplastic anemia","volume":"379","author":"Young","year":"2018","journal-title":"N Engl J Med"},{"issue":"4","key":"2021070815251428300_B3","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.hoc.2018.03.001","article-title":"The pathophysiology of acquired aplastic anemia: current concepts revisited","volume":"32","author":"Schoettler","year":"2018","journal-title":"Hematol Oncol Clin North Am"},{"issue":"11","key":"2021070815251428300_B4","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1182\/blood.V85.11.3183.bloodjournal85113183","article-title":"Fas antigen expression on CD34+ human marrow cells is induced by interferon gamma and tumor necrosis factor alpha and potentiates cytokine-mediated hematopoietic suppression in vitro","volume":"85","author":"Maciejewski","year":"1995","journal-title":"Blood"},{"issue":"1","key":"2021070815251428300_B5","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1073\/pnas.82.1.188","article-title":"Interferon is a mediator of hematopoietic suppression in aplastic anemia in vitro and possibly in vivo","volume":"82","author":"Zoumbos","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"issue":"26","key":"2021070815251428300_B6","doi-asserted-by":"crossref","first-page":"2730","DOI":"10.1182\/blood-2018-05-844928","article-title":"Macrophage TNF-\u03b1 licenses donor T cells in murine bone marrow failure and can be implicated in human aplastic anemia","volume":"132","author":"Sun","year":"2018","journal-title":"Blood"},{"issue":"9","key":"2021070815251428300_B7","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.3324\/haematol.2011.056705","article-title":"CD34+ gene expression profiling of individual children with very severe aplastic anemia indicates a pathogenic role of integrin receptors and the proapoptotic death ligand TRAIL","volume":"97","author":"Fischer","year":"2012","journal-title":"Haematologica"},{"issue":"1","key":"2021070815251428300_B8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1182\/blood-2003-02-0490","article-title":"Gene expression profiling in CD34 cells to identify differences between aplastic anemia patients and healthy volunteers","volume":"103","author":"Zeng","year":"2004","journal-title":"Blood"},{"issue":"17","key":"2021070815251428300_B9","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1182\/blood-2017-02-767731","article-title":"Origins of myelodysplastic syndromes after aplastic anemia","volume":"130","author":"Negoro","year":"2017","journal-title":"Blood"},{"key":"2021070815251428300_B10","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1182\/asheducation-2011.1.90","article-title":"Clonal evolution in aplastic anemia","volume":"2011","author":"Afable","year":"2011","journal-title":"Hematology Am Soc Hematol Educ Program"},{"issue":"17","key":"2021070815251428300_B11","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1182\/blood-2014-05-574889","article-title":"Somatic mutations identify a subgroup of aplastic anemia patients who progress to myelodysplastic syndrome","volume":"124","author":"Kulasekararaj","year":"2014","journal-title":"Blood"},{"issue":"1","key":"2021070815251428300_B12","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1182\/blood.2019000940","article-title":"Secondary myelodysplastic syndrome and leukemia in acquired aplastic anemia and paroxysmal nocturnal hemoglobinuria","volume":"136","author":"Sun","year":"2020","journal-title":"Blood"},{"issue":"9","key":"2021070815251428300_B13","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1182\/bloodadvances.2017013953","article-title":"Sustained clonal hematopoiesis by HLA-lacking hematopoietic stem cells without driver mutations in aplastic anemia","volume":"2","author":"Imi","year":"2018","journal-title":"Blood Adv"},{"issue":"1","key":"2021070815251428300_B14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1056\/NEJMoa1414799","article-title":"Somatic mutations and clonal hematopoiesis in aplastic anemia","volume":"373","author":"Yoshizato","year":"2015","journal-title":"N Engl J Med"},{"issue":"7367","key":"2021070815251428300_B15","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/nature10496","article-title":"Frequent pathway mutations of splicing machinery in myelodysplasia","volume":"478","author":"Yoshida","year":"2011","journal-title":"Nature"},{"issue":"5","key":"2021070815251428300_B16","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.ccell.2015.04.016","article-title":"Charting the \u201csplice\u201d routes to MDS","volume":"27","author":"Obeng","year":"2015","journal-title":"Cancer Cell"},{"issue":"15","key":"2021070815251428300_B17","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1182\/blood-2011-12-399337","article-title":"Frequency and prognostic impact of mutations in SRSF2, U2AF1, and ZRSR2 in patients with myelodysplastic syndromes","volume":"119","author":"Thol","year":"2012","journal-title":"Blood"},{"issue":"25","key":"2021070815251428300_B18","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1182\/blood-2013-09-381665","article-title":"The genetic basis of myelodysplasia and its clinical relevance","volume":"122","author":"Cazzola","year":"2013","journal-title":"Blood"},{"issue":"4","key":"2021070815251428300_B19","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.4049\/jimmunol.125.4.1449","article-title":"Immune suppression of hematopoiesis in aplastic anemia: activity of T-gamma lymphocytes","volume":"125","author":"Bacigalupo","year":"1980","journal-title":"J Immunol"},{"issue":"1","key":"2021070815251428300_B20","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1365-2141.1984.tb06063.x","article-title":"Analysis of lymphocyte subsets in patients with aplastic anaemia","volume":"58","author":"Zoumbos","year":"1984","journal-title":"Br J Haematol"},{"issue":"5","key":"2021070815251428300_B21","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1056\/NEJM198501313120501","article-title":"Circulating activated suppressor T lymphocytes in aplastic anemia","volume":"312","author":"Zoumbos","year":"1985","journal-title":"N Engl J Med"},{"issue":"19","key":"2021070815251428300_B22","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1056\/NEJM199105093241901","article-title":"Treatment of aplastic anemia with antilymphocyte globulin and methylprednisolone with or without cyclosporine","volume":"324","author":"Frickhofen","year":"1991","journal-title":"N Engl J Med"},{"issue":"4","key":"2021070815251428300_B23","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1182\/blood-2002-01-0035","article-title":"Intracellular interferon-gamma in circulating and marrow T cells detected by flow cytometry and the response to immunosuppressive therapy in patients with aplastic anemia","volume":"100","author":"Sloand","year":"2002","journal-title":"Blood"},{"issue":"9","key":"2021070815251428300_B24","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1007\/s00277-005-1022-8","article-title":"Expression of interferon-gamma and tumor necrosis factor-alpha in bone marrow T cells and their levels in bone marrow plasma in patients with aplastic anemia","volume":"84","author":"Dubey","year":"2005","journal-title":"Ann Hematol"},{"issue":"1","key":"2021070815251428300_B25","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1046\/j.1365-2141.2002.03592.x","article-title":"Hypoplastic myelodysplastic syndromes can be distinguished from acquired aplastic anaemia by bone marrow stem cell expression of the tumour necrosis factor receptor","volume":"118","author":"Kasahara","year":"2002","journal-title":"Br J Haematol"},{"key":"2021070815251428300_B26","article-title":"Single-cell transcriptomic landscape of human blood cells [published online ahead of print 24 August 2020]","author":"Xie","year":"2020","journal-title":"Natl Sci Rev"},{"issue":"6","key":"2021070815251428300_B27","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/j.stem.2017.05.009","article-title":"Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions","volume":"20","author":"Li","year":"2017","journal-title":"Cell Stem Cell"},{"issue":"6","key":"2021070815251428300_B28","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1038\/s41556-018-0105-4","article-title":"Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing","volume":"20","author":"Gao","year":"2018","journal-title":"Nat Cell Biol"},{"issue":"7","key":"2021070815251428300_B29","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1101\/gr.110882.110","article-title":"Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq","volume":"21","author":"Islam","year":"2011","journal-title":"Genome Res"},{"issue":"11","key":"2021070815251428300_B30","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1038\/nmeth.2639","article-title":"Smart-seq2 for sensitive full-length transcriptome profiling in single cells","volume":"10","author":"Picelli","year":"2013","journal-title":"Nat Methods"},{"issue":"1","key":"2021070815251428300_B31","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/nprot.2014.006","article-title":"Full-length RNA-seq from single cells using Smart-seq2","volume":"9","author":"Picelli","year":"2014","journal-title":"Nat Protoc"},{"issue":"4","key":"2021070815251428300_B32","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"issue":"5","key":"2021070815251428300_B33","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nbt.4096","article-title":"Integrating single-cell transcriptomic data across different conditions, technologies, and species","volume":"36","author":"Butler","year":"2018","journal-title":"Nat Biotechnol"},{"issue":"7","key":"2021070815251428300_B34","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"issue":"11","key":"2021070815251428300_B35","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1038\/nmeth.4463","article-title":"SCENIC: single-cell regulatory network inference and clustering","volume":"14","author":"Aibar","year":"2017","journal-title":"Nat Methods"},{"issue":"7731","key":"2021070815251428300_B36","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/s41586-018-0698-6","article-title":"Single-cell reconstruction of the early maternal-fetal interface in humans","volume":"563","author":"Vento-Tormo","year":"2018","journal-title":"Nature"},{"issue":"4","key":"2021070815251428300_B37","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1038\/s41596-020-0292-x","article-title":"CellPhoneDB: inferring cell-cell communication from combined expression of multi-subunit ligand-receptor complexes","volume":"15","author":"Efremova","year":"2020","journal-title":"Nat Protoc"},{"issue":"1","key":"2021070815251428300_B38","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"issue":"5","key":"2021070815251428300_B39","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.4644","article-title":"scmap: projection of single-cell RNA-seq data across data sets","volume":"15","author":"Kiselev","year":"2018","journal-title":"Nat Methods"},{"issue":"8","key":"2021070815251428300_B40","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.1093\/nar\/gkr1291","article-title":"MATS: a Bayesian framework for flexible detection of differential alternative splicing from RNA-Seq data","volume":"40","author":"Shen","year":"2012","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2021070815251428300_B41","doi-asserted-by":"crossref","first-page":"5274","DOI":"10.1038\/ncomms6274","article-title":"Dynamic analyses of alternative polyadenylation from RNA-seq reveal a 3\u2032-UTR landscape across seven tumour types","volume":"5","author":"Xia","year":"2014","journal-title":"Nat Commun"},{"issue":"4","key":"2021070815251428300_B42","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1038\/ncb3493","article-title":"Human haematopoietic stem cell lineage commitment is a continuous process","volume":"19","author":"Velten","year":"2017","journal-title":"Nat Cell Biol"},{"issue":"5","key":"2021070815251428300_B43","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1182\/blood.V46.5.735.735","article-title":"Damaged DNA in lymphocytes of aplastic anemia","volume":"46","author":"Hashimoto","year":"1975","journal-title":"Blood"},{"issue":"1","key":"2021070815251428300_B44","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13023-019-1046-0","article-title":"Genetic analyses of aplastic anemia and idiopathic pulmonary fibrosis patients with short telomeres, possible implication of DNA-repair genes","volume":"14","author":"Arias-Salgado","year":"2019","journal-title":"Orphanet J Rare Dis"},{"issue":"1","key":"2021070815251428300_B45","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1038\/s41467-018-06063-x","article-title":"Aberrant splicing and defective mRNA production induced by somatic spliceosome mutations in myelodysplasia","volume":"9","author":"Shiozawa","year":"2018","journal-title":"Nat Commun"},{"issue":"1","key":"2021070815251428300_B46","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1093\/nar\/gki158","article-title":"A large-scale analysis of mRNA polyadenylation of human and mouse genes","volume":"33","author":"Tian","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"2021070815251428300_B47","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.cell.2009.06.016","article-title":"Widespread shortening of 3\u2019UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells","volume":"138","author":"Mayr","year":"2009","journal-title":"Cell"},{"issue":"7505","key":"2021070815251428300_B48","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/nature13261","article-title":"CFIm25 links alternative polyadenylation to glioblastoma tumour suppression","volume":"510","author":"Masamha","year":"2014","journal-title":"Nature"},{"issue":"7221","key":"2021070815251428300_B49","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1038\/nature07509","article-title":"Alternative isoform regulation in human tissue transcriptomes","volume":"456","author":"Wang","year":"2008","journal-title":"Nature"},{"issue":"12","key":"2021070815251428300_B50","doi-asserted-by":"crossref","first-page":"R100","DOI":"10.1186\/gb-2005-6-12-r100","article-title":"Biased alternative polyadenylation in human tissues","volume":"6","author":"Zhang","year":"2005","journal-title":"Genome Biol"},{"issue":"3","key":"2021070815251428300_B51","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/ng.2894","article-title":"Identification of functional cooperative mutations of SETD2 in human acute leukemia","volume":"46","author":"Zhu","year":"2014","journal-title":"Nat Genet"},{"issue":"4","key":"2021070815251428300_B52","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.4049\/jimmunol.1501739","article-title":"Memory stem T cells in autoimmune disease: high frequency of circulating CD8+ memory stem cells in acquired aplastic anemia","volume":"196","author":"Hosokawa","year":"2016","journal-title":"J Immunol"},{"issue":"4","key":"2021070815251428300_B53","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1046\/j.1365-2141.2001.03026.x","article-title":"Intracellular Fas ligand is elevated in T lymphocytes in severe aplastic anaemia","volume":"114","author":"Luther-Wyrsch","year":"2001","journal-title":"Br J Haematol"},{"issue":"8","key":"2021070815251428300_B54","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1182\/blood-2011-11-390708","article-title":"Intrinsic impairment of CD4(+)CD25(+) regulatory T cells in acquired aplastic anemia","volume":"120","author":"Shi","year":"2012","journal-title":"Blood"},{"issue":"6","key":"2021070815251428300_B55","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1111\/j.1423-0410.1979.tb04511.x","article-title":"Circulating suppressor cells in aplastic anemia","volume":"36","author":"G\u00f3rski","year":"1979","journal-title":"Vox Sang"},{"key":"2021070815251428300_B56","doi-asserted-by":"crossref","first-page":"1364165","DOI":"10.1155\/2018\/1364165","article-title":"PKM2 is required to activate myeloid dendritic cells from patients with severe aplastic anemia","volume":"2018","author":"Liu","year":"2018","journal-title":"Oxid Med Cell Longev"},{"issue":"5","key":"2021070815251428300_B57","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/ni.2555","article-title":"The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells","volume":"14","author":"Gatto","year":"2013","journal-title":"Nat Immunol"},{"key":"2021070815251428300_B58","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.exphem.2018.10.003","article-title":"Interleukin-18 plays a dispensable role in murine and likely also human bone marrow failure","volume":"69","author":"Wu","year":"2019","journal-title":"Exp Hematol"},{"issue":"5","key":"2021070815251428300_B59","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.4049\/jimmunol.1801630","article-title":"Integrin binding to the trimeric interface of CD40L plays a critical role in CD40\/CD40L signaling","volume":"203","author":"Takada","year":"2019","journal-title":"J Immunol"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/138\/1\/23\/1812961\/bloodbld2020008966.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/138\/1\/23\/1812961\/bloodbld2020008966.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T22:47:38Z","timestamp":1698101258000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/138\/1\/23\/475591\/Single-cell-transcriptomics-dissects-hematopoietic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,24]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,3,24]]},"published-print":{"date-parts":[[2021,7,8]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2020008966","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,24]]}}}